Comparison of Quantitative Precipitin Techniques as Influenced by Injection Procedures

Wolfe, Harold R.; Baier, Joseph G.

doi:10.1086/physzool.11.1.30151441

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1939

1966

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“…Magnus (1908) noted that antisera specificity was lessened by continued injections of an antigen. Wolfe (1935Wolfe ( , 1936 and Wolfe and Baier (1938) emphasized the importance of the effects of injection methods on the specificity and aspecificity of the serum precipitin tests. It was shown that the type of response is greatly influenced by the method of injection, the specificity being enhanced by limiting the amount of protein injected.…”

Section: R Wolfementioning

confidence: 99%

Standardization of the Precipitin Technique and Its Application to Studies of Relationships in Mammals, Birds and Reptiles

Wolfe¹

1939

The Biological Bulletin

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Section: R Wolfementioning

confidence: 99%

Standardization of the Precipitin Technique and Its Application to Studies of Relationships in Mammals, Birds and Reptiles

Wolfe¹

1939

The Biological Bulletin

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Experimental Taxonomy: A Review of Some Techniques and Their Applications

Wright

1966

International Review of General and Experimental Zoology

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Factors Influencing the Performance of the Precipitin Test in the Determination of Blood Meals of Insects

Eligh¹

1952

Can. J. Zool.

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Certain factors influence the performance of precipitin tests when the latter are used for detection of blood meals of biting flies. Antisera stored in a liquid state in a refrigerator generally show a decrease in titer with the passage of time. Aging appears to have an adverse effect on the avidity of an antiserum. Smears of blood-engorged mosquitoes prepared by smearing of the entire insect are satisfactory for testing by the precipitin reaction. A high-titered antiserum which can be diluted is desirable for detection of host blood in mosquitoes.

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Comparison of Quantitative Precipitin Techniques as Influenced by Injection Procedures

Cited by 3 publications

References 9 publications

Standardization of the Precipitin Technique and Its Application to Studies of Relationships in Mammals, Birds and Reptiles

Standardization of the Precipitin Technique and Its Application to Studies of Relationships in Mammals, Birds and Reptiles

Experimental Taxonomy: A Review of Some Techniques and Their Applications

Factors Influencing the Performance of the Precipitin Test in the Determination of Blood Meals of Insects

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