Comparison of Quantifiler ® Trio and InnoQuant™ human DNA quantification kits for detection of DNA degradation in developed and aged fingerprints

A review of trace “Touch DNA” deposits: Variability factors and an exploration of cellular composition

“…These varied techniques have led to some of the conflicting fragment size results in plasma and fetal cfDNA, while the fragmentation in other sources of cfDNA has not been investigated at length. Quantitative PCR to estimate degradation based on short and long fragment ratios can inform researchers about the condition of touch cfDNA [172,173,174], although anything PCR-based risks overlooking fragments without primer binding sites, which is a concern if cfDNA in touch samples is fragmented similarly to that in plasma. When saliva samples were separated into their cellular and cell-free components, the latter required significantly higher input for reliable DNA typing results, possibly due to this fragmentation of the salivary cfDNA making successful amplification more challenging [164].…”

Validation of Reduced Reagent Volumes in the Implementation of the Quantifiler® Trio Quantification Kit

“…The C T values of the standard samples obtained using the two methods are compared in Table . The Quantifiler ® Trio Quantification Kit estimates the quantities of T.LA (long autosomal amplicons, 214 bp), T.SA (short autosomal amplicons, 80 bp), and T.Y (Y targets) . Degradation can be evaluated based on the ratio of T.LA to T.SA .…”

Forensic proteomics

“…This often results in failure to obtain useable STR-profiles and this has driven the development of low copy number DNA processing methods to maximize the amount of template present in analysis [308]. Because the J o u r n a l P r e -p r o o f fingermarks are on exposed two-dimensional surfaces, they are subject to environmental degradation [305,[309][310][311][312]. In a scenario where one particular type of information may not provide sufficient information, such as a partial STR-profile, incorporation of additional information types may provide additional identifying information or investigative context [143,148,[313][314][315].…”

“…The sex chromosome amelogenin loci are in all commercial forensic STR-based analysis kits [5]. Current forensic sex-estimation methods rely on both STR-typing and quantitative PCR, such as used in the Quantifiler ® HP and Quantifiler ® Trio DNA Quantification Kits [312,343,[346][347][348][349].…”