Comparison of pulsed‐field gel electrophoresis and enterobacterial repetitive intergenic consensus PCR and biochemical tests to characterize Lactococcus garvieae

Abstract

Biochemical test, pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) were used to compare 42 strains of Lactococcus garvieae isolated from different regions of Turkey, Italy, France and Spain. Twenty biotypes of L. garvieae were formed based on 54 biochemical tests. ERIC-PCR of genomic DNA from different L. garvieae strains resulted in amplification of multiple fragments of DNA in sizes ranging between 200 and 5000 bp with various band intensities. After cutting DNA with ApaI restriction enzyme and running on the PFGE, 11–22 resolvable bands ranging from 2 to 194 kb were observed. Turkish isolates were grouped into two clusters, and only A58 (Italy) strain was connected with Turkish isolates. Similarities between Turkish, Spanish, Italian and French isolates were <50% except 216-6 Rize strain. In Turkey, first lactococcosis occurred in Mugla, and then, it has been spread all over the country. Based on ERIC-PCR, Spanish and Italian strains of L. garvieae were related to Mugla strains. Therefore, after comparing PFGE profiles, ERIC-PCR profiles and phenotypic characteristics of 42 strains of L. garvieae, there were no relationships found between these three typing methods. PFGE method was more discriminative than the other methods.

Safety assessment, genetic relatedness and bacteriocin activity of potential probiotic Lactococcus lactis strains from rainbow trout (Oncorhynchus mykiss, Walbaum) and rearing environment

“…Although rep-PCR was not effective for fingerprint grouping of the 75 L. lactis strains evaluated in this study, this technique has been successfully used by other authors for genetic profiling of lactococci [56][57][58]. On the other hand, ERIC-PCR has been used before to study the genetic variability of L. lactis and L. garvieae [59,60]. In our work, ERIC-PCR fingerprinting revealed three well-defined and highly divergent groups within the 75 L. lactis strains, where G1 and G3 are the most phylogenetically distant and, interestingly, correspond to strains from the early fry and adult stages, respectively.…”

Bacterial and Parasitic Pathogens Isolated from Some Wild Cyprinid Fishes

“…Yersinia ruckeri and Lactococcus sp. are commonly seen pathogens in the aquaculture farms constructed in the rivers and dam lakes of the Eastern Black Sea region of Turkey [27,28]. In last few years, the vaccination improved on fish against these two bacteria has decreased the extensity of the diseases.…”

Molecular characterization of microbial contaminants isolated from Umbilical Cord Blood Units for transplant

“…This molecular tool has been widely used to rule out clonal relationships between bacteria from the same genus and species isolated from a specific area. 29,30 Correlation of the antimicrobial resistance pattern and plasmid profiles with clonality in contaminants strains is limited, especially in the hospital environment.…”