Comparison of proteomic profiles from the testicular tissue of males with impaired and normal spermatogenesis

Liang, Jingang; Zheng, Yian; Zeng, Weihong; Shaofen, Yang; Wang, Yujiang; Yu, Xingsu; Zhang, Xiqian

doi:10.1080/19396368.2020.1846822

Cited by 17 publications

(10 citation statements)

References 41 publications

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“…5E), implicating this subset of genes as direct targets. Among the 76 proteins, 21 were RNA-binding proteins (RBPs), 10 of which were suggested to be involved in spermatogenesis (Kuroda et al 2000, Yang et al 2012, Chapman et al 2013, Fukuda et al 2013, Legrand et al 2019, Sechi et al 2019, Liang et al 2021, Tian and Petkov 2021, Wang et al 2022, Xu et al 2022); 8 were involved in mitochondrial function; and 4 were calcium channel activity-related proteins (Fig. 5F).…”

Section: Resultsmentioning

confidence: 99%

Deficiency of IQCH causes male infertility in humans and mice

Ruan

Zhou

Yang

et al. 2023

Preprint

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IQ motif-containing proteins can be recognized by calmodulin (CaM) and are essential for many biological processes. However, the role of IQ motif-containing proteins in spermatogenesis is largely unknown. In this study, we identified a loss-of-function mutation in the novel gene IQ motif-containing H (IQCH) in a Chinese family with male infertility, characterized by a cracked flagellar axoneme and abnormal mitochondrial structure. To verify the function of IQCH, Iqch-knockout mice were generated by CRISPR-Cas9 technology which reproduced the human phenotypes. Mechanistically, IQCH can bind to CaM and then regulate the expression of RNA-binding proteins (especially HNRPAB), which are indispensable for spermatogenesis. Collectively, this study firstly unveiled the function of IQCH, expanded the role of IQ motif-containing proteins in reproductive processes, and provided important guidance for genetic counseling and gene diagnosis for male infertility.

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Section: Resultsmentioning

confidence: 99%

Deficiency of IQCH causes male infertility in humans and mice

Ruan

Zhou

Yang

et al. 2023

Preprint

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“…The ribosome-associated pathway includes ribosomal components as well as small nucleolar RNA (snoRNA), ribosomal proteins, and non-ribosomal proteins. The ribosome pathway can cause reproductive toxicity by regulating gene expression, leading to spermatogenesis disorders [ 23 , 24 ]. In addition to occurring during protein synthesis, ribosome collisions often occur when cells are under stress.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomic Analyses for the Optimization of Thawing Regimes during Conventional Cryopreservation of Mature and Immature Human Testicular Tissue

Pei,

Todorov,

Cao

et al. 2023

IJMS

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Cryopreservation of human testicular tissue, as a key element of anticancer therapy, includes the following stages: saturation with cryoprotectants, freezing, thawing, and removal of cryoprotectants. According to the point of view existing in “classical” cryobiology, the thawing mode is the most important consideration in the entire process of cryopreservation of any type of cells, including cells of testicular tissue. The existing postulate in cryobiology states that any frozen types of cells must be thawed as quickly as possible. The technologically maximum possible thawing temperature is 100 °C, which is used in our technology for the cryopreservation of testicular tissue. However, there are other points of view on the rate of cell thawing, according to how thawing should be carried out at physiological temperatures. In fact, there are morphological and functional differences between immature (from prepubertal patients) and mature testicular tissue. Accordingly, the question of the influence of thawing temperature on both types of tissues is relevant. The purpose of this study is to explore the transcriptomic differences of cryopreserved mature and immature testicular tissue subjected to different thawing methods by RNA sequencing. Collected and frozen testicular tissue samples were divided into four groups: quickly (in boiling water at 100 °C) thawed cryopreserved mature testicular tissue (group 1), slowly (by a physiological temperature of 37 °C) thawed mature testicular tissue (group 2), quickly thawed immature testicular tissue (group 3), and slowly thawed immature testicular tissue (group 4). Transcriptomic differences were assessed using differentially expressed genes (DEG), the Kyoto Encyclopedia of Genes and Genomes (KEGG), gene ontology (GO), and protein–protein interaction (PPI) analyses. No fundamental differences in the quality of cells of mature and immature testicular tissue after cryopreservation were found. Generally, thawing of mature and immature testicular tissue was more effective at 100 °C. The greatest difference in the intensity of gene expression was observed in ribosomes of cells thawed at 100 °C in comparison with cells thawed at 37 °C. In conclusion, an elevated speed of thawing is beneficial for frozen testicular tissue.

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“…Infertility occurs in up to 12% of all male population [ 1 ]. The etiology of male infertility varies, but spermatogenic dysfunction, featured by the existence of defects in spermatogenesis function of testes, is the most common component in male infertility [ 2 ]. Although semen analysis roughly assesses the occurrence of spermatogenic dysfunction, its final diagnosis and grading assessment rely on pathological evaluation, especially for non-obstructive azoospermia (NOA) patients.…”

Section: Introductionmentioning

confidence: 99%

Identification and validation of CCL2 as a potential biomarker relevant to mast cell infiltration in the testicular immune microenvironment of spermatogenic dysfunction

et al. 2023

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Background Spermatogenic dysfunction is an important cause of azoospermia. Numerous studies have focused on germ-cell-related genes that lead to spermatogenic impairment. However, based on the immune-privileged characteristics of the testis, the relationship of immune genes, immune cells or immune microenvironment with spermatogenic dysfunction has rarely been reported. Results Using integrated methods including single-cell RNA-seq, microarray data, clinical data analyses and histological/pathological staining, we found that testicular mast cell infiltration levels were significantly negatively related to spermatogenic function. We next identified a functional testicular immune biomarker, CCL2, and externally validated that testicular CCL2 was significantly upregulated in spermatogenic dysfunctional testes and was negatively correlated with Johnsen scores (JS) and testicular volumes. We also demonstrated that CCL2 levels showed a significant positive correlation with testicular mast cell infiltration levels. Moreover, we showed myoid cells and Leydig cells were two of the important sources of testicular CCL2 in spermatogenic dysfunction. Mechanistically, we drew a potential “myoid/Leydig cells-CCL2-ACKR1-endothelial cells-SELE-CD44-mast cells” network of somatic cell–cell communications in the testicular microenvironment, which might play roles in spermatogenic dysfunction. Conclusions The present study revealed CCL2-relevant changes in the testicular immune microenvironment in spermatogenic dysfunction, providing new evidence for the role of immunological factors in azoospermia.

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Comparison of proteomic profiles from the testicular tissue of males with impaired and normal spermatogenesis

Cited by 17 publications

References 41 publications

Deficiency of IQCH causes male infertility in humans and mice

Deficiency of IQCH causes male infertility in humans and mice

Comparative Transcriptomic Analyses for the Optimization of Thawing Regimes during Conventional Cryopreservation of Mature and Immature Human Testicular Tissue

Identification and validation of CCL2 as a potential biomarker relevant to mast cell infiltration in the testicular immune microenvironment of spermatogenic dysfunction

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