Comparison of proteins involved in chondroitin sulfate utilization by three colonic Bacteroides species

Lipeski, Lauren; Guthrie, Ellen P.; O'Brien, M.; Kotarski, Susan F.; Salyers, Abigail A.

doi:10.1128/aem.51.5.978-984.1986

Cited by 10 publications

(3 citation statements)

References 14 publications

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“…Some human colonic Bacteroides species such as Bacteroides thetaiotaomicron have evolved complex regulated systems for catabolism of mucopolysaccharides (7,8,11). Mucopolysaccharides such as chondroitin sulfate and hyaluronic acid are released into the lumen of the mammalian small intestine during sloughing of mucosal cells, but it is not known how much mucopolysaccharide actually enters the colon each day.…”

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confidence: 99%

Importance of mucopolysaccharides as substrates for Bacteroides thetaiotaomicron growing in intestinal tracts of exgermfree mice

Salyers

Pajeau

McCarthy

1988

Appl Environ Microbiol

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We used two approaches to determine whether the mucopolysaccharide chondroitin sulfate is an important source of carbon and energy for Bacteroides thetaiotaomicron in the intestinal tracts of germfree mice. First, we tested the ability of three mutants that grew poorly or not at all on chondroitin sulfate to colonize the intestinal tract of a germfree mouse and to compete with wild-type B. thetaiotaomicron in this model system. One mutant (CG10) was rapidly outcompeted by the wild type. However, since this mutant was unable to grow on chondroitin sulfate because it could not grow on N-acetyl-galactosamine, one of its monosaccharide components, this mutant might also be unable to utilize glycoprotein mucins. Two mutants (46-1 and 46-4) were isolated that grew poorly on chondroitin sulfate but normally on both component sugars. One of them was outcompeted by the wild type, but the percent wild type increased more slowly than with CG10. In one experiment, the percent wild type never reached 100%. The other (46-4) was not outcompeted by the wild type. These results indicate that, although chondroitin sulfate may be a carbon source in the animal, it is not of major importance. Our second approach was to determine by immunoblot analysis whether a 28-kilodalton outer membrane protein that is produced by B. thetaiotaomicron only when it is grown on chondroitin sulfate or hyaluronic acid was being produced at induced level by B. thetaiotaomicron growing in the ceca of exgermfree mice. There was no evidence for induction of this protein in vivo. Thus, the immunoblot results are consistent with results of the mutant competition experiments.

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confidence: 99%

Importance of mucopolysaccharides as substrates for Bacteroides thetaiotaomicron growing in intestinal tracts of exgermfree mice

Salyers

Pajeau

McCarthy

1988

Appl Environ Microbiol

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“…CS supports the overgrowth of Bacteroides sp. strain 3452A (an unnamed DNA homology group), B. ovatus , and B. thetaiotaomicron (Lipeski et al, 1986 ) in the colon and can promote the growth of B. thetaiotaomicron , which is an SSB, and Desulfovibrio piger , a species of sulfate-reducing bacteria (SRB) (Rey et al, 2013 ). SRB may thus thrive if supported by a food chain-like lifestyle, i.e., SRB growth depends on sulfate-containing CS.…”

Section: Proinflammatory Activity Of Cs-promoted Ssb and Anti- Inflammentioning

confidence: 99%

Akkermansia muciniphila May Determine Chondroitin Sulfate Ameliorating or Aggravating Osteoarthritis

Wang

Huang

et al. 2017

Front. Microbiol.

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Chondroitin sulfate (CS) has shown either ameliorating or aggravating effects on osteoarthritis (OA) in separately conducted clinical trials. Because CS is usually administered orally, it should be affected by or would impact on the individual gut microbiota. Evidence is accumulating that CS can nourish sulfatase-secreting bacteria (SSB) and sulfate-reducing bacteria (SRB). To decipher how can an individual gut microbiota determine the clinical values of CS for treatment on OA, we suggest here that CS would give distinct outcomes for OA treatment depending on Akkermansia muciniphila, a gut commensal probiotic bacterial species as optimal presence albeit also behaving as mucus-eroding bacteria (MEB) when abundant presence. Briefly, CS would ameliorate OA if A. muciniphila is present due to without overgrowth of SSB and SRB, whereas CS would aggravate OA if A. muciniphila is absent because of failure in or lack of competition with abundant SSB and SRB. By noting such a frequently ignored phenomenon, we urge the development of non-orally administering CS to minimize its side-effects and extend it to other medicinal applications.

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“…cies have enzymes that have physical and biochemical properties similar to chondroitinase I and CSase II of B. thetaiotaomicron (7). Although the enzymes from the different species are very similar, they are probably not identical because antiserum that detects CSase II from B. thetaiotaomicron does not react with that from either of the other two Bacteroides species (7). Also, the cloned CSase II gene from B. thetaiotaomicron (on pUSH1) does not hybridize with chromosomal DNA from the other Bacteroides species unless low-stringency conditions are used (7).…”

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confidence: 99%

A deletion in the chromosome of Bacteroides thetaiotaomicron that abolishes production of chondroitinase II does not affect survival of the organism in gastrointestinal tracts of exgermfree mice

Salyers

Guthrie

1988

Appl Environ Microbiol

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Bacteroides thetaiotaomicron, an obligate anaerobe normally found in high concentrations in the human colon, is one of the few colon bacteria that can ferment host mucopolysaccharides such as chondroitin sulfate. Previously, we found that a directed insertional mutation in the gene that codes for the chondroitinase II gene of B. thetaiotaomicron did not affect growth on chondroitin sulfate despite the fact that chondroitinase II accounts for 70% of the total cellular chondroitinase activity. Thus, the chondroitinase II gene did not seem to contribute significantly to growth on chondroitin sulfate when the bacteria were grown in laboratory medium. To determine whether this enzyme is important for bacteria growing in the intestinal tract, we tested the ability of a strain that does not produce chondroitinase II to colonize the intestinal tracts of germfree mice and to compete with wild-type B. thetaiotaomicron. The mutant used in these experiments carried a 0.5-kilobase deletion in the chondroitinase II gene and was constructed so that, unlike the original insertion mutant, it contained no exogenous DNA. The deletion mutant colonized the intestinal tracts of germfree mice at the same levels as the wild type. When a mixture of the deletion mutant and wild type was used to colonize germfree mice, the percent wild type, measured by colony hybridization with the deleted 0.5-kilobase fragment as the hybridization probe, did not rise to 100% even after periods as long as 9 weeks. In most experiments, the percent wild type did not rise significantly above the percent in the original mixture. Thus, it appears that the chondroitinase II does not make a major contribution to the survival of B. thetaiotaomicron in the gastrointestinal tract of a germfree mouse.

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Comparison of proteins involved in chondroitin sulfate utilization by three colonic Bacteroides species

Cited by 10 publications

References 14 publications

Importance of mucopolysaccharides as substrates for Bacteroides thetaiotaomicron growing in intestinal tracts of exgermfree mice

Importance of mucopolysaccharides as substrates for Bacteroides thetaiotaomicron growing in intestinal tracts of exgermfree mice

Akkermansia muciniphila May Determine Chondroitin Sulfate Ameliorating or Aggravating Osteoarthritis

A deletion in the chromosome of Bacteroides thetaiotaomicron that abolishes production of chondroitinase II does not affect survival of the organism in gastrointestinal tracts of exgermfree mice

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