Comparison of primary human cytotoxic T-cell and natural killer cell responses reveal similar molecular requirements for lytic granule exocytosis but differences in cytokine production

Epstein-Barr Virus Coinfection in Children Boosts Cytomegalovirus-Induced Differentiation of Natural Killer Cells

“…CMV, in particular, can drive the differentiation of highly mature (also known as late or terminally differentiated) cytotoxic T cells, phenotypically characterized by the lack of CD28 and expression of CD57 surface markers (3). High expression of CD57 has been linked to elevated lytic granule content in T cells (4,5). In line with this, highly differentiated CD8…”

Adaptive NK cells can persist in patients with GATA2 mutation depleted of stem and progenitor cells

“…NK cells and cytotoxic T lymphocytes (CTLs) use the same machinery for target cell killing, yet relative expression levels of granule constituents differ. 29 Investigating healthy CMV-seropositive donors (n 5 16) with sizeable adaptive NK-cell populations by intracellular flow cytometry, we found that adaptive PLZF 2 as compared with canonical PLZF 1 NK cells display lower expression of perforin and granzyme A and increased expression of granzyme B ( Figure 3A; supplemental Figure 2A). As such, expression levels of granzyme A and B in …”

“…For functional analyses, lymphocytes were stimulated, surface stained with antibodies and a fixable dead cell stain, as previously described. 24,29 In experiments measuring cytokine production, GolgiPlug (BD Biosciences) was added during stimulation. Flow cytometry data acquisition and analyses are detailed in supplemental Methods.…”

Autoimmunity, hypogammaglobulinemia, lymphoproliferation, and mycobacterial disease in patients with activating mutations in STAT3

“…18 Evaluation of T-and NK-cell responses is described in detail elsewhere. 17,19 For the assessment of T-cell activation and degranulation, fresh mononuclear cells were stimulated for 6 hours with anti-CD3, anti-CD28, and anti-CD49d (BD Biosciences). For NK cell degranulation, cytokine and cytotoxicity assays, fresh mononuclear cells or fluorescence-activated cell-sorted CD3 …”