Comparison of Preventive and Therapeutic Transplantations of Allogeneic Mesenchymal Stem Cells in Healing of the Distal Femoral Growth Plate Cartilage Defects in Miniature Pigs

Plánka, Ladislav; Starý, David; Hlučilová, Jana; Klíma, Jiří; Jančář, J.; Křen, Leoš; Lorenzová, Jana; Urbanová, Lucie; Crha, Michal; Srnec, Robert; Dvořák, Milan; Gál, Petr; Nečas, Alois

doi:10.2754/avb200978020293

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…Furthermore, it has been demonstrated that BMSCs can stimulate angiogenesis, suppress the immunoreaction, and inhibit fiber tissue formation . Altogether, the evidence suggests that BMSCs maybe more suitable as seed cells in the treatment of GP injuries (Planka et al, 2009). As for MSCs, another problem that has been addressed is whether autogenous MSCs are superior to allogeneic MSCs.…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Enlightenment of Growth Plate Regeneration Based on Cartilage Repair Theory: A Review

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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The growth plate (GP) is a cartilaginous region situated between the epiphysis and metaphysis at the end of the immature long bone, which is susceptible to mechanical damage because of its vulnerable structure. Due to the limited regeneration ability of the GP, current clinical treatment strategies (e.g., bone bridge resection and fat engraftment) always result in bone bridge formation, which will cause length discrepancy and angular deformity, thus making satisfactory outcomes difficult to achieve. The introduction of cartilage repair theory and cartilage tissue engineering technology may encourage novel therapeutic approaches for GP repair using tissue engineered GPs, including biocompatible scaffolds incorporated with appropriate seed cells and growth factors. In this review, we summarize the physiological structure of GPs, the pathological process, and repair phases of GP injuries, placing greater emphasis on advanced tissue engineering strategies for GP repair. Furthermore, we also propose that three-dimensional printing technology will play a significant role in this field in the future given its advantage of bionic replication of complex structures. We predict that tissue engineering strategies will offer a significant alternative to the management of GP injuries.

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Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Enlightenment of Growth Plate Regeneration Based on Cartilage Repair Theory: A Review

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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“…For cells and chondrogenic molecules to have an effect at the site of physeal injury, they must be delivered locally by a material that can serve as a temporary scaffold while new tissue forms. Materials used in the treatment of physeal injuries have included collagen I and II, 86 fibrin glue, 87 hyaluronate-collagen-fibrin composites, 65,92,93 collagenchitin scaffolds, 66,67,111 agarose, 61,88 chitin, 64 gelatin, 69,90 and PLGA. 63,107 The outcomes of the studies using these materials are outlined in Table 1.…”

Section: Biomaterial-based Approachesmentioning

confidence: 99%

Regenerative Medicine Approaches for the Treatment of Pediatric Physeal Injuries

Shaw

Erickson

Bryant

et al. 2018

Tissue Engineering Part B: Reviews

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The physis, or growth plate, is a cartilaginous region at the end of children's long bones that serves as the primary center for longitudinal growth and characterizes the immature skeleton. Musculoskeletal injury, including fracture, infection, malignancy, or iatrogenic damage, has risk of physeal damage. Physeal injuries account for 30% of pediatric fractures and may result in impaired bone growth. Once damaged, cartilage tissue within the physis is often replaced by unwanted bony tissue, forming a "bony bar" that can lead to complications such as complete growth arrest, angular or rotational deformities, and altered joint mechanics. Children with a bony bar occupying <50% of the physis usually undergo bony bar resection and insertion of an interpositional material, such as a fat graft, to prevent recurrence and allow the surrounding uninjured physeal tissue to restore longitudinal bone growth. Clinical success for this procedure is <35% and often the bony bar and associated growth impairments return. Children who are not candidates for bony bar resection due to a physeal bar occupying >50% of their physis undergo corrective osteotomy or bone lengthening procedures. These approaches are complex and have variable success rates. As such, there is a critical need for regenerative approaches to not only prevent initial bony bar formation but also regenerate healthy physeal cartilage following injury. This review describes physeal anatomy, mechanisms of physeal injury, and current treatment options with associated limitations. Furthermore, we provide an overview of the current research using cell-based therapies, growth factors, and biomaterials in the different animal models of injury along with strategic directions for modulating intrinsic injury pathways to inhibit bony bar formation and/or promote physeal tissue formation. Pediatric physeal injuries constitute a unique niche within regenerative medicine for which there is a critical need for research to decrease child morbidity related to this injurious process.

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“…Použití kmenových buněk v uvedené indikaci bylo jen otázkou času po informacích o nadějné účinnosti při použití v neurologii (36) nebo kardiologii (2). Proběhly první studie s pozitivními výsledky ve světě (1) i na domácím pracovišti (6,31,32,33).…”

Section: Diskuseunclassified

Treatment of a Bone Bridge by Transplantation of Mesenchymal Stem Cells and Chondrocytes in a Composite Scaffold in Pigs. Experimental Study

Plánka¹,

Nečas²,

Crha³

et al. 2011

Acta Chir. orthop. Traum. Cech.

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PURPOSE OF THE STUDYThe presented experimental study describes the results of using a combination of allogeneic mesenchymal cells (MSCs) with chondrocytes (CHCs) and a novel scaffold based on type I collagen and chitosan fibres. This biocomposite was transplanted into a defect produced by excision of a bone bridge to induce new cartilaginous tissue formation. The left femur was treated by transplantation into a defect of distal epiphysis; the right femur with implantation of the scaffold only served as control. A better therapeutic result was therefore expected in the left femur -the reduction of growth and angular deformities, and the histological finding of a tissue similar to the cartilage excised from the left femur.. MATERIAL AND METHODSThe miniature pig was selected as an experimental model and 10 pigs were used. Mesenchymal stem cells derived from femoral bone marrow and chondrocytes derived from a sample harvested from the non-weight-bearing articular surface of the distal end of the femur were cultured in medium. The novel scaffold was based on collagen containing chitosan nanofibres. To make manipulation during implantation easier, the cilindrical scaffolds after lyophilisation were again placed in 96-well plates for seeding. The scaffolds before implantation were seeded with 2x106 allogeneic MSCs and 1x106 allogeneic CHCs. The outcomes of treatment were assessed by measuring the length of bone and the degree of distal femoral valgus deformity, and by the histological findings obtained (properties and maturity of the newly-formed tissue, detection of type II collagen, PAS reaction). RESULTSThe right and left legs were examined for longitudinal bone growth and the valgus angle and compared. The treated left leg showed a higher average value for longitudinal growth than the untreated right leg (p = 0.004). The average degree of angular deformity was lower in the left leg than in the right leg (p = 0.008). The microscopic findings showed that a tissue similar to hyaline cartilage was more frequently present in the femoral bone defect of the left leg, as compared with that of the right leg. Type II collagen was detected more frequently and at higher amounts on the left than the right side (p = 0.033). The PAS reaction was positive in all left limbs, with a high degree of positivity in 80 % of them, while this was not achieved in any of the right limbs (p = 0.001). DISCUSSIONThe use of stem cells in the indication reported here has only been the matter of time since the information on encouraging results in neurology and cardiology was published. First studies with positive results have soon been reported. The initial hydrogel scaffolds were based on tissue adhesives. However, they were not stable enough and were difficult to handle during surgery. In further studies, therefore, the use was made of a three-dimensional scaffold with a self-supporting structure of collagen fibres. This structure also facilitated its hydrodynamic seeding with MSCs and CHCs, which is an effective and sparing procedure for the tr...

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Comparison of Preventive and Therapeutic Transplantations of Allogeneic Mesenchymal Stem Cells in Healing of the Distal Femoral Growth Plate Cartilage Defects in Miniature Pigs

Cited by 9 publications

References 27 publications

Enlightenment of Growth Plate Regeneration Based on Cartilage Repair Theory: A Review

Enlightenment of Growth Plate Regeneration Based on Cartilage Repair Theory: A Review

Regenerative Medicine Approaches for the Treatment of Pediatric Physeal Injuries

Treatment of a Bone Bridge by Transplantation of Mesenchymal Stem Cells and Chondrocytes in a Composite Scaffold in Pigs. Experimental Study

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