Comparison of prevalence of <i>Felis catus</i> papillomavirus type 2 in squamous cell carcinomas in cats between Taiwan and Japan

Abstract

Felis catus papillomavirus (FcaPV), especially type 2 (FcaPV2) is considered as one of the causative agents in squamous cell carcinoma (SCC) in cats. However, our previous study detected FcaPV3 and FcaPV4, but not FcaPV2 in feline SCCs collected in Japan, suggesting that the prevalence of FcaPV2 in SCC may vary depending on geographic locations. To evaluate this hypothesis, two conventional PCR reactions targeting E1 and E7 genes were performed to detect FcaPV2 in feline SCC samples collected in Taiwan and Japan. While 46.9 % (23/49) of feline SCC cases from Taiwan were PCR positive for FcaPV2, only 8.6 % (3/35) cases from Japan were positive. Our result suggests that the prevalence of FcaPV2 in feline SCCs may depend on the region.

Investigation of multiple <i>Felis catus</i> papillomavirus types (-1/-2/-3/-4/-5/-6) DNAs in feline oral squamous cell carcinoma: a multicentric study

“…This is the first study specifically testing the presence of each FcaPV type in such a numerous sample of FOSCC and cell lines. A possible limitation may be DNA fragmentation occurring in FFPE samples, which might have influenced PCR results; however, in agreement with previous studies, our data confirm that the use of type-specific primers amplifying short viral sequences allows detection of FcaPVs in FFPE-FOSCCs, likely overcoming this issue and ensuring a better yield with respect to consensus oligos amplifying longer sequences [6,18,21,22,29]. FcaPV-2 was found to be the most prevalent and confirmed as the unique viral type associated with SCCF2/3 cell lines [5].…”

“…FcaPV-2 was found to be the most prevalent and confirmed as the unique viral type associated with SCCF2/3 cell lines [5]. These data are in line with the growing evidence supporting a role for FcaPV-2 in development of a subset of FOSCC, such as detection of viral DNA and transcriptional activity in tumor samples and its biological similarities with mucosal HPV-16 in experimental models [4,5,26,29]; nevertheless, FcaPV-2-associated FOSCC has been proposed as a spontaneous animal model of human counterpart [1].…”

“…However, our recent work pointed out that the use of type-specific primers amplifying short sequences increased PCR performance, leading to detection of FcaPV-2 in 10/32 (31%) FOSCC samples in Italy [6]. Moreover, studies conducted with similar methods in Japan found FcaPV-2 in 12/28 (42.9%) tumors, strengthening the possibility of a biologically significant association [29].…”

Papillomaviruses in Domestic Cats

“…However, a smaller proportion of SCCs have been found to contain FacPV3, FcaPV4, or FcaPV6 DNA sequences [25,29,54,57,61]. As with BISCs, there is some evidence of regional variation with FcaPV3 detected most frequently in SCCs from cats in Japan [86]. Whether or not the subsequent behavior of the SCC is influenced by the type of PV that caused the lesion is currently unknown.…”

“…Sequences from FcaPV2 DNA were also detected in 11 of 19 (58%) OSCCs in a recent study of cats from Taiwan. No non-neoplastic oral samples were included in this study [86].…”