Comparison of pregnancy rates with transfer of in vivo produced embryos derived using multiple ovulation and embryo transfer (MOET) with in vitro produced embryos by somatic cell nuclear transfer (SCNT) in the dromedary camel (Camelus dromedaries)

Vettical, Binoy S.; Hong, Seungbum; Umer, Muhammad; Wani, Nisar Ahmad

doi:10.1016/j.anireprosci.2019.106132

Cited by 9 publications

(4 citation statements)

References 36 publications

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“…With this preliminary finding it appears conclusive that if there exist any differences between camel breeds and cloning efficiency it is greatly overshadowed by individual variation (Table 1 ). Our efficiency data appears to vary from other reports 5 , 28 although seasonal variations may play a partial role, they should be considered when making comparisons 29 . The oocyte conditions, both daily and throughout the season may additionally be a factor in our total results.…”

Section: Discussioncontrasting

confidence: 65%

Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

Olsson¹,

Tinson²,

Shamsi³

et al. 2021

Sci Rep

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Cloning, through somatic cell nuclear transfer (SCNT), has the potential for a large expansion of genetically favorable traits in a population in a relatively short term. In the present study we aimed to produce multiple cloned camels from racing, show and dairy exemplars. We compared several parameters including oocyte source, donor cell and breed differences, transfer methods, embryo formation and pregnancy rates and maintenance following SCNT. We successfully achieved 47 pregnancies, 28 births and 19 cloned offspring who are at present healthy and have developed normally. Here we report cloned camels from surgical embryo transfer and correlate blastocyst formation rates with the ability to achieve pregnancies. We found no difference in the parameters affecting production of clones by camel breed, and show clear differences on oocyte source in cloning outcomes. Taken together we demonstrate that large scale cloning of camels is possible and that further improvements can be achieved.

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Section: Discussioncontrasting

confidence: 65%

Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

Olsson¹,

Tinson²,

Shamsi³

et al. 2021

Sci Rep

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“…No pregnancy loss was observed after 90 days of pregnancy in in vivo matured oocyte group, whereas 20% (1 out of 5 pregnancies) abortion was observed at 168 days of gestation. Vettical et al [ 41 ] reported a similar pattern of pregnancy loss in camel following the transfer of SCNT-derived embryos; they observed 18% pregnancy loss within 60 days of pregnancy, and 7% (1 out of 15 pregnancies) of pregnancy loss after 90 days of gestation. Determine the exact causes of higher embryonic loss in camel is difficult, as numerous factors may complicate the diagnosis.…”

Section: Discussionmentioning

confidence: 86%

The Resurrection of Mabrokan: Production of Multiple Cloned Offspring from Decade-Old Vitrified Tissue Collected from a Deceased Champion Show Camel

Hossein¹,

Son³

et al. 2021

Animals

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Somatic cell nuclear transfer (SCNT) provides a unique opportunity to reproduce animals with superior genetics. Viable cell lines are usually established from tissues collected by biopsy from living animals in the SCNT program. In the present study, tissues were collected and preserved from a suddenly deceased champion camel. We established cell lines from these decade-old tissues and used them as nuclear donors. After 42 h of in vitro maturation, 68.00 ± 2.40% of oocytes reached the metaphase II (M II) stage while 87.31 ± 2.57% in vivo collected oocytes were matured at collection (p < 0.05). We observed a higher blastocyst formation rate when in vivo matured oocytes (43.45 ± 2.07%) were used compared to in vitro matured oocytes (21.52 ± 1.74%). The live birth rate was 6.45% vs. 16.67% for in vitro and in vivo matured oocytes, respectively. Microsatellite analysis of 13 camel loci revealed that all the SCNT-derived offspring were identical to each other and with their somatic cell donor. The present study succeeded in the resurrection of 11 healthy offspring from the decade-old vitrified tissues of a single somatic cell donor individual using both in vitro and in vivo matured oocytes.

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“…The findings of this case report will support the growing embryo transfer practices in camel breeding. A fully grown hatching or hatched blastocyst is usually transferred to the recipient in multiple ovulation embryo transfer and cloning programs ( 18 ). Proper care and gentle handling of blastocyst could prevent the development of monoamniotic twins.…”

Section: Discussionmentioning

confidence: 99%

Case report: Spontaneous abortion of monoamniotic twins at the third trimester of pregnancy in Camelus dromedarius

Hossein,

Son,

Jeong

et al. 2023

Front. Vet. Sci.

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Monoamniotic twins develop when a blastocyst spontaneously splits its progenitor cells, and each group of progenitor cells independently grows to become an individual. It is the rarest type of twin pregnancy and usually has significant developmental or congenital abnormalities, a higher rate of abortion, perinatal morbidity, and mortality. There is no information regarding monoamniotic twins in livestock species. Here, we reported a spontaneous abortion of monoamniotic twins in a dromedary camel at 278 days of gestation. Gonadorelin acetate (100 μg) was injected intramuscularly to induce ovulation in the recipient. A 7 days-old embryo produced by somatic cell nuclear transfer was transferred transcervically to the recipient. Early pregnancy was confirmed by an elevated level of serum progesterone followed by ultrasonography at 22 and 44 days after embryo transfer. A single sac was observed on 22 days while twins were evident 44 days after embryo transfer. Pregnancy was periodically monitored by the tail-up phenomenon. A ruptured fetal sac was observed on the ground having two fetuses. On autopsy, full-grown fetuses were found. Their bodies were separated. There was no congenital anomaly or any malformation in the fetuses. According to the reported chronology in human twins, we hypothesized that the blastocyst splitted before 13 days as it was monoamniotic and not conjoined. If the embryo splits within 4 to 8 days, it develops two amniotic sacs, and splitting after 13 days develops conjoined fetuses. To the authors’ knowledge, this is the first reported case of monoamniotic twin abortion in dromedary camels. This report will increase awareness among practicing veterinarians and camel breeders about twin abortions.

show abstract

Comparison of pregnancy rates with transfer of in vivo produced embryos derived using multiple ovulation and embryo transfer (MOET) with in vitro produced embryos by somatic cell nuclear transfer (SCNT) in the dromedary camel (Camelus dromedaries)

Cited by 9 publications

References 36 publications

Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

The Resurrection of Mabrokan: Production of Multiple Cloned Offspring from Decade-Old Vitrified Tissue Collected from a Deceased Champion Show Camel

Case report: Spontaneous abortion of monoamniotic twins at the third trimester of pregnancy in Camelus dromedarius

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