Comparison of Potentials of Stem Cells Isolated from Tendon and Bone Marrow for Musculoskeletal Tissue Engineering

Tan, Qi; Lui, Pauline Po Yee; Rui, Yun Feng; Wong, Yin Mei

doi:10.1089/ten.tea.2011.0362

Cited by 177 publications

(161 citation statements)

References 39 publications

(59 reference statements)

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“…As in equine MSCs, CD 29 and CD 44 expressions are found consistently in canine (34,35,51,52), ovine (53), and porcine (54) MSCs. Corresponding to the equine antigen expression profile as well, CD 73 could not be detected in canine, rabbit, or sheep MSCs (35,55,56), while rat tendon derived, feline and bovine neonatal cell sources and caprine bone marrow-derived MSCs expressed this antigen (57)(58)(59)(60). CD 73 protein expression could also be detected in bone marrow derived MSCs from mice but only in moderate amounts (61).…”

Section: Discussionmentioning

confidence: 92%

Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: An approach toward a standardized definition

et al. 2014

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Horses are an approved large animal model for therapies of the musculoskeletal system. Especially for tendon disease where cell-based therapy is commonly used in equine patients, the translation of achieved results to human medicine would be a great accomplishment. Immunophenotyping of equine mesenchymal stromal cells (MSCs) remains the last obstacle to meet the criteria of the International Society for Cellular Therapy (ISCT) definition of human MSCs. Therefore, the surface antigen expression of CD 29, CD 44, CD 73, CD 90, CD 105, CD 14, CD 34, CD 45, CD 79a, and MHC II in equine MSCs from adipose tissue, bone marrow, umbilical cord blood, umbilical cord tissue, and tendon tissue was analyzed using flow cytometry. Isolated cells from the different sources and donors varied in their expression pattern of MSC-defining antigens. In particular, CD 90 and 105 showed most heterogeneity. However, cells from all samples were robustly positive for CD 29 and CD 44, while being mostly negative for CD 73 and the exclusion markers CD 14, CD 34, CD 45, CD 79a and MHC II. Furthermore, it was evident that enzymes used for cell detachment after in vitro-culture affected the detection of antigen expression. These results emphasize the need of standardization of MSC isolation, culturing, and harvesting techniques. As the equine MSCs did not meet all criteria the ISCT defined for human MSCs, further investigations for a better characterization of the cell type should be conducted. V C 2014 International Society for Advancement of Cytometry

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Section: Discussionmentioning

confidence: 92%

Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: An approach toward a standardized definition

et al. 2014

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“…5,33 Along this line, a very recent study reported an in-depth comparison of the phenotype and the regenerative potential of rat TDSCs and BMSCs. 31 Interestingly, the authors found that TDSCs possess a higher proliferative rate and multilineage differentiation potential than BMSCs, therefore concluding that TDSCs are a very promising stem cell source for musculoskeletal tissue engineering.…”

mentioning

confidence: 99%

Isolation and Characterization of 2 New Human Rotator Cuff and Long Head of Biceps Tendon Cells Possessing Stem Cell–Like Self-Renewal and Multipotential Differentiation Capacity

et al. 2013

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Background: Stem cell therapy is expected to offer new alternatives to the traditional therapies of rotator cuff tendon tears. In particular, resident, tissue-specific, adult stem cells seem to have a higher regenerative potential for the tissue where they reside. Hypothesis: Rotator cuff tendon and long head of the biceps tendon possess a resident stem cell population that, when properly stimulated, may be induced to proliferate, thus being potentially usable for tendon regeneration. Study Design: Controlled laboratory study. Methods: Human tendon samples from the supraspinatus and the long head of the biceps were collected during rotator cuff tendon surgeries from 26 patients, washed with phosphate-buffered saline, cut into small pieces, and digested with collagenase type I and dispase. After centrifugation, cell pellets were resuspended in appropriate culture medium and plated. Adherent cells were cultured, phenotypically characterized, and then compared with human bone marrow stromal cells (BMSCs), as an example of adult stem cells, and human dermal fibroblasts, as normal proliferating cells with no stem cell properties. Results: Two new adult stem cell populations from the supraspinatus and long head of the biceps tendons were isolated, characterized, and cultured in vitro. Cells showed adult stem cell characteristics (ie, they were self-renewing in vitro, clonogenic, and multipotent), as they could be induced to differentiate into different cell types—namely, osteoblasts, adipocytes, and skeletal muscle cells. Conclusion: This work demonstrated that human rotator cuff tendon stem cells and human long head of the biceps tendon stem cells can be isolated and possess a high regenerative potential, which is comparable with that of BMSCs. Moreover, comparative analysis of the sphingolipid pattern of isolated cells with that of BMSCs and fibroblasts revealed the possibility of using this class of lipids as new possible markers of the cell differentiation status. Clinical Relevance: Rotator cuff and long head of the biceps tendons contain a stem cell population that can proliferate in vitro and could constitute an easily accessible stem cell source to develop novel therapies for tendon regeneration.

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“…Spontaneous osteogenesis was also reported in other mesenchymal stem cells (MSCs), such as BMSCs [26,27], which might be due to endogenous BMP expression in MSCs [28]. Our previous study showed that TDSCs also exhibited higher osteogenic differentiation potential in osteogenic medium supplemented with dexamethosone, β-glycerolphosphate and ascorbic acid compared with BMSCs isolated from the same animal [29]. Our results thus suggest that TDSCs might be an attractive source for TBJ repair upon endogenous and/or exogenous BMP-2 stimulation, as bone formation is one of the key determinants of successful TBJ regeneration.…”

Section: Discussionmentioning

confidence: 87%

Higher BMP receptor expression and BMP-2-induced osteogenic differentiation in tendon-derived stem cells compared with bone-marrow-derived mesenchymal stem cells

Rui

Lui

Lee

et al. 2011

International Orthopaedics (SICOT)

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Purpose Surgical reattachment of tendon to bone often fails due to regeneration failure of the specialised tendon-bone junction (TBJ). The use of mesenchymal stem cells for TBJ regeneration has been reported with promising results. Tendonderived stem cells (TDSCs) with high proliferative and multilineage differentiation potential have been isolated. As stem cells residing in tendons, TDSCs can be considered a new cell source for TBJ repair. Bone morphogenic protein 2 (BMP-2) is a potent osteogenic factor with roles in normal bone healing and pathological ectopic bone formation in soft tissues. The use of BMP-2 to promote TBJ repair has been well reported. This study aimed to compare TDSCs to the gold standard bonemarrow-derived mesenchymal stem cells (BMSCs) with respect to osteogenic response to BMP-2 in vitro. Method The clonogenicity and multi-differentiation potential of TDSCs and BMSCs were identified by colony-formingunit assay, osteogenic, adipogenic and chondrogenic differentiation assays. Their osteogenic response to BMP-2 in vitro was examined by alkaline phosphatase (ALP) cytochemical staining, ALP activity assay and Alizarin red S staining of calcium nodule formation. Messenger RNA (mRNA) and BMP receptor (types IA, IB and II) protein expression were examined by quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) and Western blotting. Results Our results showed that both TDSCs and BMSCs exhibited stem cell properties, including clonogenicity and multi-differentiation potential. TDSCs expressed higher mRNA and protein levels of BMP receptors IA, IB and II. They also exhibited higher osteogenic differentiation with and without BMP-2 stimulation compared with BMSCs. Conclusions TDSCs with/without BMP-2 might be an attractive source for TBJ repair compared with BMSCs.

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Comparison of Potentials of Stem Cells Isolated from Tendon and Bone Marrow for Musculoskeletal Tissue Engineering

Cited by 177 publications

References 39 publications

Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: An approach toward a standardized definition

Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: An approach toward a standardized definition

Isolation and Characterization of 2 New Human Rotator Cuff and Long Head of Biceps Tendon Cells Possessing Stem Cell–Like Self-Renewal and Multipotential Differentiation Capacity

Higher BMP receptor expression and BMP-2-induced osteogenic differentiation in tendon-derived stem cells compared with bone-marrow-derived mesenchymal stem cells

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