Comparison of polymerase chain reaction-based genotyping of Helicobacter pylori by direct polymerase chain reaction from biopsies and cultures from patients with dyspepsia in Nigeria

Aim: Helicobacter pylori is usually detected based on hematoxylin–eosin (H–E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H–E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H–E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H–E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H–E or IHC (p < 0.001). Conclusion: H–E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.

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Helicobacter pylori and antibiotic resistance in the pediatric population: an update from Vietnam

Cisarò¹,

Pizzol

Calvo

2020

Minerva Med

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Comparison of polymerase chain reaction-based genotyping of Helicobacter pylori by direct polymerase chain reaction from biopsies and cultures from patients with dyspepsia in Nigeria

Cited by 7 publications

References 35 publications

Helicobacter pylori antibiotic resistance and correlation with cagA motifs and homB gene

Helicobacter pylori antibiotic resistance and correlation with cagA motifs and homB gene

Detection ofHelicobacter Pyloriin Gastric Cancer Tissue through Histopathology, Immunohistochemistry and Real-Time PCR

Helicobacter pylori and antibiotic resistance in the pediatric population: an update from Vietnam

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