Comparison of polyclonal and monoclonal antibodies to Actinomyces and Arachnia species

Rp, Happonen; Arstila, Pertti; Viander, M.; Söderling, Eva; Viljanen, Matti K.

doi:10.1111/j.1600-0722.1987.tb01821.x

Cited by 1 publication

(1 citation statement)

References 15 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such studies will be given much greater precision by the use of monoclonal antibodies which can be used as a primary coating in ELISA assays to "capture" only the specific antigen under test. Monoclonal antibodies now exist against a variety of antigens from bacteria incriminated in the aetiology of periodontal diseases such as F. nucleatum (Bird & Seymour 1987), B. gingivalis (Gmur & Guggenheim 1983, Chen et al 1986, Gmur et al 1988), B. forsythus and W. recta (Werner-Feldmayer et al 1988) and Aa (Place et al 1988) as well as those not currently incriminated such as Actinomyces and Arachnia species (Happonen et al 1987). Once the antigens are better characterised both in terms of their use as specific markers of the humoral antibody responses to distinguish between health and disease or between active or inactive disease and once their virulence properties (or lack of such) have also been estabHshed, then the way will be clear to investigate the role of antibodies in the modulation of virulence and thus the infectious mechanisms of the periodontal diseases.…”

Section: Discussionmentioning

confidence: 99%

Specific antibody responses to subgingival plaque bacteria as aids to the diagnosis and prognosis of destructive periodontitis*

Wilton

Johnson

Curtis

et al. 1991

J Clinic Periodontology

View full text Add to dashboard Cite

We have reviewed the recent literature on the humoral immune responses to a variety of subgingival plaque bacterial species in patients with destructive periodontal diseases. We do not feel that the information presently available on the specific antibody responses to proposed pathogens such as Bacteroides gingivalis and Actinobacillus actinomycetemcomitans allows antibody responses to be diagnostic. All control subjects without periodontal destruction have antibodies to candidate pathogens but the generally higher levels in patients are not sufficiently elevated to be diagnostic. Nor can they be used to predict the initiation of disease or the onset of new episodes of destruction where disease had previously occurred. Successful treatment of patients may lead to lower levels of antibodies to some organisms, including possible pathogens, and thus support a given species in the aetiopathogenesis of disease. It appears that unsuccessful treatment may be accompanied by continuing high antibody levels to some organisms and further studies may enable this observation to be used to monitor therapy. There is some evidence from serological studies that each destructive episode may be induced by a different bacterial species or consortium. The start of studies using single antigens and the techniques of molecular biology will provide not only antibody-based diagnostic methods but also allow us to determine which bacterial antigens are virulence factors and thus the role of the antibody responses, whether protective or damaging, in the periodontal diseases.

show abstract