Comparison of Platelet Concentrates Obtained with Two Different Plateletpheresis Programs of MCS^®+

Abstract

Background: The new software, revision C2 of the MCS+ (Haemonetics) with continuous filtration provides reduced leukocyte filtration rates of 8–10 ml/min as the collection and storage bags hang in parallel and both are positioned slightly above the level of the leukocyte filter. Filtration is performed during the entire collection procedure. Prolonged active line sensor control enhances the surge mechanism and thus collection efficiency. Return flow rates are adapted to donors’ body weight and observed by the newly introduced citrate re-infusion control. Material and Methods: With regard to these possible advantages, we evaluated in a prospective, randomized trial plateletpheresis with revision C2 in terms of leukocyte contamination, platelet collection efficiency, and donors’ safety. Results were compared with data obtained from platelet collection using the old software, revision C. Cell counts were measured electronically, residual white blood cells were determined by flow cytometry, and pH was measured with a pH meter. Results: The platelet yield (p < 0.05) and the platelet collection efficiency (p < 0.05) are significantly higher with revision C2 (yield: median 4.11 × 10¹¹, range 3.31–6.09 × 10¹¹; collection efficiency: median 68%, range 62–82%) as compared to revision C (yield: median 3.80 × 10¹¹, range 2.76–5.05 × 10¹¹; collection efficiency: median 59%, range 49–69%). Continuously filtered platelet concentrates contained 75% less leukocytes (median 0.27 × 10⁵, range 0.2–1.3 × 10⁵; lower detection limit 0.2 × 10⁵) than platelet concentrates derived with revision C (median 1.1 × 10⁵, range 0.2–56.7 × 10⁵; lower detection limit 0.2 × 10⁵) (p < 0.05). With the new software, citrate reactions were observed in 6/26 donors compared to 10/29 with the old software. Conclusions: Our data indicate that plateletpheresis with revision C2 is a safe procedure and provides a ready-to-use, high-quality platelet product with low leukocyte contamination.

Fuji surge technique and continuous in‐line filtration to improve the quality of single donor platelet concentrates

“…Compared to our previous data, PLT yield was higher with the new software revision [7]. The present data are in line with those of Stiegler et al, who reported on a median CE of 68% and a PLT yield 4.11 · 10 11 [27]. CE obtained with the discontinuous flow separator MCS+ are similar to those of the Amicus blood cell separator [5][6][7] and higher than those of the AS.TEC 204 and Cobe Spectra devices [2,3,5].…”

“…They concluded that better WBC removal was obtained with continuous filtration than with postcollection filtration. Stiegler and co-workers reported on a significantly lower median WBC contamination of 0.028 · 10 6 in SDPs leukoreduced with a low filtration rate compared with SDPs, which were filtered with a high filtration rate [27]. Our present study confirms these findings as the WBC numbers of the SDPs were lower than in our previous study with postcollection filtration resulting in a mean WBC contamination of 0.088 ± 0.178 · 10 6 [7].…”

“…Reduction of the filtration rate results in more time for interaction of the WBCs with the filter material, thereby improving the leukoreduction process. Several authors found a decrease in leukocyte contamination of SDPs using slow filtration rates compared with fast filtration rates (Table III) [24,27,28]. Using continuous filtration, Holme et al found that 100% of the SDPs leuko-reduced with the LRF-XL filter had WBC counts <1 · 10 5 [24].…”