Comparison of Planktonic and Biofilm Cultures of
            <i>Pseudomonas fluorescens</i>
            DSM 8341 Cells Grown on Fluoroacetate

Heffernan, Barry; Murphy, Cormac D.; Casey, Eoin

doi:10.1128/aem.01530-08

Cited by 36 publications

(25 citation statements)

References 37 publications

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Section: Discussionmentioning

confidence: 99%

“…Gradients of respiratory activity in the biofilm samples were not visible using CTC and DAPI in combination; however, these stains have previously been proven to highlight the gradients where present in biofilm systems (Heffernan et al 2009). There is no fundamental reason why the RDR could not be used to view gradients in oxygen or nutrients in a biofilm, if present, provided the cor-rect staining methods are employed.…”

Section: Analysis Of Biofilm Thicknessmentioning

confidence: 98%

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Characterization of a modified rotating disk reactor for the cultivation of Staphylococcus epidermidis biofilm

Cotter

O’Gara

Stewart

et al. 2010

Journal of Applied Microbiology

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Aims: The purpose of this study was to develop a system that would allow biofilms to be cultivated under strictly defined conditions in terms of dissolved oxygen, fluid shear and to assess whether the method was suitable for the detection of respiratory activity stratification in biofilm samples. Methods: The system is a modified version a commercially available laboratory biofilm reactor and incorporates a number of features such as the provision of defined levels of dissolved oxygen, constant average shear, enhanced gas–liquid mass transfer, aseptic operation and the ability to remove biofilm for ex situ analysis during or after continuous cultivation. Conclusions: The system was shown to be effective for the characterization of the effects of dissolved oxygen on a pure culture of Staphylococcus epidermidis. The versatility of the system offers the potential for cultivating pure culture biofilm in defined, controlled conditions and facilitates a range of analyses that can be performed ex situ. Significance and Impact of the Study: The ability to provide strict regulation of environmental conditions and enhanced transfer of oxygen to the biofilm during cultivation are important, first because oxygen is known to regulate biofilm development in several micro‐organisms and second because many conventional biofilm cultivation systems may not provide adequate oxygen supply to the biofilm.

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Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Biofilm Thicknessmentioning

confidence: 98%

Characterization of a modified rotating disk reactor for the cultivation of Staphylococcus epidermidis biofilm

Cotter

O’Gara

Stewart

et al. 2010

Journal of Applied Microbiology

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“…Likewise, this amendment of wastewater with acetate (400 mg·L -1 ) has been shown to decrease start-up times over the use of fumarate (600 mg·L -1 ), glucose (350 mg·L -1 ), or wastewater . Another method to improve start-up times may be to operate the reactor in continuous flow mode, thereby encouraging the adherence of bacteria to the electrode in addition to preventing localized depletion of electron donors by planktonic cells (Heffernan et al 2009). Adding phosphate buffer to increase the conductivity and limit the growth of non-exoelectrogens has also been shown to be effective Min et al 2008;Nam et al 2010), though increasing the buffer beyond 100 mM has demonstrated a negative impact on start-up times .…”

Section: Start-up Proceduresmentioning

confidence: 99%

Microbial Fuel Cells: From Fundamentals to Wastewater Treatment Applications

Gao¹,

Lesnik²,

Bermek³

et al. 2015

Anaerobic Biotechnology

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Microbial fuel cells (MFCs) have continued to offer the potential to increase treatment efficiency, power generation, and energy recovery during wastewater treatment, and have garnered considerable research interest over the past 15 years. For the time being, the potential of this technology remains unfulfilled as scaling-up MFC reactors has presented a significant engineering challenge. The recent development of materials and designs that reduce fabrication costs in addition to maintaining high performance at increased reactor sizes has brought MFCs a step closer to practical application. The optimization of operating conditions has led to additional improvements in performance and has further increased the feasibility of these technologies. Herein, the fundamental concepts governing MFC performance are detailed along with highlighting developments in materials, designs, and operating conditions with the hope of promoting future scale-up efforts and a more sustainable wastewater treatment infrastructure.

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“…Biofilm constitutes a protected mode of growth that allows microorganisms to survive, even in hostile environments, their phenotypes, physiology and behaviour being significantly different from their planktonic counterparts (Stewart and Franklin, 2008;Heffernan et al, 2009). Not only do bacteria in biofilm differ from planktonic bacteria of the same species, but also bacteria in mono species biofilm demonstrate vast heterogeneity in terms of metabolism, gene expression and physiology due to the different conditions on different microlocations (Koh et al, 2007;Vlamakis et al, 2008).…”

Section: Introductionmentioning

confidence: 98%

The control of biofilm formation by hydrodynamics of purified water in industrial distribution system

Florjanič

Kristl

2011

International Journal of Pharmaceutics

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Comparison of Planktonic and Biofilm Cultures of Pseudomonas fluorescens DSM 8341 Cells Grown on Fluoroacetate

Cited by 36 publications

References 37 publications

Characterization of a modified rotating disk reactor for the cultivation of Staphylococcus epidermidis biofilm

Characterization of a modified rotating disk reactor for the cultivation of Staphylococcus epidermidis biofilm

Microbial Fuel Cells: From Fundamentals to Wastewater Treatment Applications

The control of biofilm formation by hydrodynamics of purified water in industrial distribution system

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