Comparison of photoautotrophic and mixotrophic cultivation of microalgae Messastrum gracile (Chlorophyceae) in alternative culture media

Abstract

Growth and biological conditions of Messastrum gracile were evaluated to compare the effect of photoautotrophic and mixotrophic cultivation on the increase of biomass production and chemical conditions cultured in macrophyte and commercial culture media. The growth rate (k) of M. gracile was different in the culture media, higher in mixotrophic cultivation for Lemna minor culture medium, whilst to Eichhornia crassipes and NPK culture media were higher in photoautotrophic cultivation. Mean lipid contents in photoautotrophic cultivation were 8.2% biomass dry weight, whereas they reached 19% biomass dry weight in mixotrophic cultivation. Protein contents were below 48% biomass dry weight in photoautotrophic cultivation and 30% biomass dry weight in mixotrophic cultivation. Messastrum gracile cultured in macrophyte culture media (E. crassipes and L. minor) and NPK culture medium provided satisfactory results with regard to lipid and protein contents in mixotrophic and photoautotrophic cultivations, respectively. Lipid and protein contents in alternative media were higher or similar to the CHU12 commercial culture medium.

Mixotrophic cultivation of Chlorella vulgaris in sugarcane molasses preceding nitrogen starvation: Biomass productivity, lipid content, and fatty acid analyses

“…[21][22][23][24][25][26][27] Such wastes are generally available at low/no cost and can be valorized as organic carbon sources for the mixotrophic cultivation of microalgae. 28,29 In this way, treatment of potentially hazardous agro-industrial wastes and sequestration of atmospheric CO 2 can be achieved concomitantly in raising highly productive oleaginous microalgal biomass mixotrophically for the production of biofuels. 30,31 It has been evident…”

“…16,[33][34][35] and Tetraselmis gracilis. 16,28,29,[36][37][38][39] Thus, keeping in view the significance of mixotrophic cultivation, the present study was designed to raise pure culture of C. vulgaris using molasses as a novel organic carbon source. The mixotrophically raised microalgal biomass was kept under nitrogen starvation and then processed for the extraction and analysis of fatty acids.…”

“…kessleri, C. sorokiniana , C. vulgaris , Desmodesmus ( D .) brasiliensis, D. spinosus , Graesiella sp., Haematococcus pluvialis , Messastrum gracile , Micractinium inermum , Monoraphidium sp., Nannochloropsis sp., Scenedesmus (S.) quadricauda and Tetraselmis gracilis 16,28,29,36‐39 . Thus, keeping in view the significance of mixotrophic cultivation, the present study was designed to raise pure culture of C. vulgaris using molasses as a novel organic carbon source.…”

“…Various agro‐industrial wastes are produced in massive proportions throughout the world and usually dumped in the ambient environment as point/nonpoint source pollutants 21‐27 . Such wastes are generally available at low/no cost and can be valorized as organic carbon sources for the mixotrophic cultivation of microalgae 28,29 . In this way, treatment of potentially hazardous agro‐industrial wastes and sequestration of atmospheric CO 2 can be achieved concomitantly in raising highly productive oleaginous microalgal biomass mixotrophically for the production of biofuels 30,31 .…”

Phycoremediation of phenol‐polluted petro‐industrial effluents and its techno‐economic values as a win‐win process for a green environment, sustainable energy and bioproducts

“…2014; Sipaúba‐Tavares et al . 2020). Consequently, the mixotrophic algal mode of growth is recommendable to gain the advantage of obtaining large biomass yield with high contents of valuable products; lipids, carbohydrates, proteins, food supplements; nontoxic dyes, and at the same time, achieve cost‐effective reclamation and treatment of phenol‐polluted effluents.…”