Comparison of phosphofructokinases in submandibular glands of immature and adults rats

Hamano, Emiko; Yamazaki, Takao; Saito, Masao; Kawashima, Haruo; Ozeki, Toyokazu; Furuyama, Shunsuke

doi:10.1016/0305-0491(89)90152-1

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…On the other hand, the activity of PFK-1, the enzyme that phosphorylates fructose-6-phophate, may be regulated by a series of allosteric effectors, substrates and products 28 as well as by Fructose-2,6-bisphosphate 13 . In the SMG of rats, PFK-1 is sinergically regulated by ATP, fructose-6-phosphate and fructose-2,6-bisphosphate 12,25 . This metabolite (fructose-2,6-bisphosphate) is a potent effector in the regulation of the allosteric nature of PFK-1.…”

Section: Numbermentioning

confidence: 99%

Effect of unilateral incisor tooth amputation on the glucose metabolism in submandibular glands of rats following sympathetic and parasympathetic denervation

Almeida-De-Faria

Bauer

Nicolau³

2000

Pesqui. Odontol. Bras.

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The autonomic nervous system is of major importance in the regulation of physiological functions of the salivary glands, including the sialadenotrophic process. It is well known that the secretory function as well as other functions in the salivary glands depend upon the energy produced in the gland. The acini volume density, some enzymes of the glucose metabolism, such as hexokinase, phosphofructokinase, pyruvate kinase, glucose-6-phosphate dehydrogenase and lactate dehydrogenase were evaluated following sympathetic and parasympathetic denervation of submandibular glands of rats, submitted to unilateral incisor amputation, five days after denervation. Sympathectomized glands submitted to amputation showed a reduction in the activity of hexokinase and phosphofructokinase-1, varying from 27.5 to 36.7 % for hexokinase and from 22.8 to 38.4 % for phosphofructokinase-1. Although no variation was observed in the enzymatic activity of parasympathectomized animals, the acini volume density showed a significant decrease.

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Section: Numbermentioning

confidence: 99%

Effect of unilateral incisor tooth amputation on the glucose metabolism in submandibular glands of rats following sympathetic and parasympathetic denervation

Almeida-De-Faria

Bauer

Nicolau³

2000

Pesqui. Odontol. Bras.

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Activity, distribution and regulation of phosphofructokinase in salivary gland of rats with streptozotocin-induced diabetes

Nicolau¹,

Souza

Nogueira³

2006

Braz. oral res.

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Although the influence of diabetes on salivary glands is well studied, it still presents conflicting results. In this work, the regulation of the phosphofructokinase-1 enzyme (PFK-1) was studied utilizing the salivary glands of rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (60 mg/Kg of body weight) in rats (180-200 g). The animals were killed 30 days after the induction of diabetes and the submandibular and parotid salivary glands were used. Hyperglycemia was evaluated by blood sugar determination. The distribution of PFK-1 between the soluble and cytoskeleton fractions, the phosphate content of PFK-1, the content of fructose-2,6-bisphosphate and the activity of the PFK-2 enzyme were determined. The calculated relative glandular weight showed a higher value for the parotid gland in comparison with the control, but not for the submandibular gland. The activity of PFK-1 expressed per gland showed no variation between diabetic and control animals. However, considering the specific activity, the soluble enzyme presented a value 50% higher than that of the control and the cytoskeleton bound form increased by 84% compared to the control. For the parotid gland, no difference in the specific activity between diabetic and control animals was observed. On the other hand, the activity per gland of the soluble enzyme increased in the diabetic animals. The phosphate content of PFK-1 increased in the submandibular and parotid glands of diabetic rats. Both the content of fructose-2,6-bisphosphate and the active form of PFK-2 were reduced in the diabetic glands. In conclusion, the increase in the activity of PFK-1 observed in the salivary glands of rats with streptozotocin-induced diabetes does not seem to be due to its modulator fructose-2,6-bisphosphate.

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Comparison of phosphofructokinases in submandibular glands of immature and adults rats

Cited by 2 publications

References 24 publications

Effect of unilateral incisor tooth amputation on the glucose metabolism in submandibular glands of rats following sympathetic and parasympathetic denervation

Effect of unilateral incisor tooth amputation on the glucose metabolism in submandibular glands of rats following sympathetic and parasympathetic denervation

Activity, distribution and regulation of phosphofructokinase in salivary gland of rats with streptozotocin-induced diabetes

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