Comparison of Petrifilm and Colilert methods for<i>E. coli</i>enumeration in recreational water

Kleinheinz, Gregory T.; Busse, Kimberly M.; Gorman, Wendy L.; McDermott, Colleen M.

doi:10.1080/07438141.2012.743492

Cited by 4 publications

(1 citation statement)

References 14 publications

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“…Various risks can be faced by the community when consuming or using water that is exposed to total coliform and E. Coli bacteria (Cronin et al, 2017;Kleinheinz et al, 2012). The well in this study is located in the middle of a densely populated settlement, actively used as a water reserve by residents.…”

Section: Water Quality Sanitation Dense Urban Settlementsmentioning

confidence: 98%

Water Sanitation Quality Assessment for Hand-Dug Wells

Sari,

Anggoro,

Sunoko

et al. 2024

RGSA

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Purpose: Public toilet sanitation facilities need to be analyzed for water sanitation quality. This research aims to explain the benefits of hand-well water for sanitation in densely populated communal environments. Theoretical framework: This article presents literature support regarding sanitation, water quality, and pollutant index in Indonesia, regarding water sanitation conditions in dense urban organizations. Method: Comprehensive data on the quality of water sanitation used by people in dense settlements. Data was collected in the form of water as a laboratory test from 24 random dug wells in four sub-districts in Bandung City and analyzed by the regulations of the Minister of Health of the Republic of Indonesia. Results and Discussion: The research results show that an average of 75% of air wells are contaminated based on water sanitation quality and pollutant index based on Minister of Health Regulation Number 32 of 2017 and Number 2 of 2023 concerning Indonesian Environmental Health and Water Health Quality Standards. Research Implications: Our findings are important for policymakers, health workers, public health practitioners, and so that the community provides public toilet facilities that can be monitored and receive government attention to improve their quality. Originality/Value: The novelty of this research lies in that it presents a comprehensive assessment of water quality indices and physical, chemical, and microbiological parameters of hand wells in dense urban organizations.

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Section: Water Quality Sanitation Dense Urban Settlementsmentioning

confidence: 98%

Water Sanitation Quality Assessment for Hand-Dug Wells

Sari,

Anggoro,

Sunoko

et al. 2024

RGSA

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Towards rapid on-site phage-mediated detection of generic Escherichia coli in water using luminescent and visual readout

Burnham

Anany

et al. 2014

Anal Bioanal Chem

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Wild-type T4 bacteriophage and recombinant reporter lac Z T4 bacteriophage carrying the β-galactosidase gene were used for detection of generic Escherichia coli by monitoring the release of β-galactosidase upon phage-mediated cell lysis. The reaction was performed on a paper-based portable culture device to limit the diffusion of reagents and, hence, increase the sensitivity of the assay, and to avoid handling large sample volumes, making the assay suitable for on-site analysis. Chromogenic (chlorophenol red-β-D-galactopyranoside, CPRG) and bioluminescent (6-O-β-galactopyranosyl-luciferin, Beta-Glo(®)) β-galactosidase substrates were tested in the assay. Water samples were first filtered through 0.45-μm pore size filters to concentrate bacteria. The filters were then placed into the paper-based device containing nutrient medium and incubated at 37 °C for 4 h. Bacteriophage with the respective indicator substrate was added to the device, and signal (color, luminescence) development was recorded with a digital camera, luminometer, or luminescence imaging device. It was demonstrated that as low as 40 or <10 colony-forming units (cfu) ml(-1) of E. coli can be detected visually within 8 h when wild-type T4 bacteriophage or recombinant lacZ T4 bacteriophage were used in the assay, respectively. Application of the bioluminescent β-galactosidase substrate allowed reliable detection of <10 cfu ml(-1) within 5.5 h. The specificity of the assay was demonstrated using a panel of microorganisms including Aeromonas hydrophila, Enterobacter cloacae, E. coli, and Salmonella Typhimurium.

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Improved sensitive fluorescent/visible dual detection count plate for mold and yeast in food

Ntakatsane²,

Wang³

et al. 2021

Food Control

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Comparison of Petrifilm and Colilert methods forE. colienumeration in recreational water

Cited by 4 publications

References 14 publications

Water Sanitation Quality Assessment for Hand-Dug Wells

Water Sanitation Quality Assessment for Hand-Dug Wells

Towards rapid on-site phage-mediated detection of generic Escherichia coli in water using luminescent and visual readout

Improved sensitive fluorescent/visible dual detection count plate for mold and yeast in food

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