Comparison of PCR-ISH and in situ PCR in cytodiagnosis specimens. A basic study on these methods and their clinical application.

Kina, Katsunari; Ishi, Kazuhisa; Okazaki, Taro; Kazama, Reiko; Koyatsu, Junichi; Saitoh, Akira; Suzuki, Fujihiko; Nakamura, Haruji

doi:10.5795/jjscc.35.32

Cited by 2 publications

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“…Furthermore, the sensitivity of in situ PCR was improved by PCR being performed on tissue specimens. In previous studies we demonstrated that in situ PCR was more able to detect HPV‐DNA than ISH in cervical smears, 37 and in the cervical squamous neoplasm of the tissue specimens 8 . Hence, based on the detailed survey of the literature and the advantages of in situ PCR, our results might be close to the true rate of HPV‐DNA detection in tumor cells in cervical adenocarcinoma.…”

Section: Discussionmentioning

confidence: 89%

Human papillomavirus localization in cervical adenocarcinoma and adenosquamous carcinoma using in situ polymerase chain reaction: Review of the literature of human papillomavirus detection in these carcinomas

Ogura¹,

Ishi²,

Matsumoto³

et al. 2006

Pathology International

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Many studies have suggested that human papillomavirus (HPV) infection plays an important role in the carcinogenesis of the cervical adenocarcinoma. However, the prevalence of HPV infection in cervical adenocarcinoma and adenosquamous carcinoma varies among the studies. Cervical adenocarcinoma (24 cases) and adenosquamous carcinoma (16 cases), including the underlying non-neoplastic epithelium were examined for HPV-DNA using in situ polymerase chain reaction (PCR), which enabled visualization of the localization on a glass slide. In adenocarcinoma, HPV-DNA was found in 13 cases (54%) and in eight cases in underlying non-neoplastic epithelium, resulting in a total of 21 positive cases (88%). In adenosquamous carcinoma, HPV-DNA was detected in 12 cases (75%) and and the HPV-DNA localization of each component was pure adenocarcinoma, 28.6%; mixed, 54.5%; and pure squamous cell carcinoma, 83.3%. In the underlying non-neoplastic epithelium, HPV-DNA was found more frequently in the squamous epithelium (73.3%) than the cervical glands (6.3%). In conclusion, HPV-DNA was detected in 54% of adenocarcinoma, and the rate was elevated by HPV localization in the underlying non-neoplastic epithelium. HPV infection in the underlying squamous epithelium might be related to the carcinogenesis, even in cervical adenocarcinoma. HPV-DNA localization was different in each component of adenosquamous carcinoma.

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Section: Discussionmentioning

confidence: 89%