Comparison of PCR assays to detect Toxoplasma gondii oocysts in green-lipped mussels (Perna canaliculus)

Coupe, Alicia; Howe, Laryssa; Shapiro, Karen; Roe, Wendi D.

doi:10.1007/s00436-019-06357-z

Cited by 12 publications

(7 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These PCR methods display high sensitivity but might lack in specificity as previously evidenced [ 22 , 166 ]. In fact, qPCR targeting the B1 gene and 529RE without probes cross-reacted with Sarcocystidae members [ 167 ]. Thus, powerful discrimination techniques are necessary to avoid false positive results and confirm species identity [ 22 , 37 , 121 ].…”

Section: Resultsmentioning

confidence: 99%

Contamination of Soil, Water, Fresh Produce, and Bivalve Mollusks with Toxoplasma gondii Oocysts: A Systematic Review

et al. 2022

View full text Add to dashboard Cite

Toxoplasma gondii is a major foodborne pathogen capable of infecting all warm-blooded animals, including humans. Although oocyst-associated toxoplasmosis outbreaks have been documented, the relevance of the environmental transmission route remains poorly investigated. Thus, we carried out an extensive systematic review on T. gondii oocyst contamination of soil, water, fresh produce, and mollusk bivalves, following the PRISMA guidelines. Studies published up to the end of 2020 were searched for in public databases and screened. The reference sections of the selected articles were examined to identify additional studies. A total of 102 out of 3201 articles were selected: 34 articles focused on soil, 40 focused on water, 23 focused on fresh produce (vegetables/fruits), and 21 focused on bivalve mollusks. Toxoplasma gondii oocysts were found in all matrices worldwide, with detection rates ranging from 0.09% (1/1109) to 100% (8/8) using bioassay or PCR-based detection methods. There was a high heterogeneity (I2 = 98.9%), which was influenced by both the sampling strategy (e.g., sampling site and sample type, sample composition, sample origin, season, number of samples, cat presence) and methodology (recovery and detection methods). Harmonized approaches are needed for the detection of T. gondii in different environmental matrices in order to obtain robust and comparable results.

show abstract

Section: Resultsmentioning

confidence: 99%

Contamination of Soil, Water, Fresh Produce, and Bivalve Mollusks with Toxoplasma gondii Oocysts: A Systematic Review

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The pooled seroprevalence of anti-T. gondii antibody from humans came from studies that focused mainly on immunocompetent individuals, HIV+ patients, and pregnant women [8,54,57,60,62,63] as well as a few studies on blood donors and children [56,61]. Overall, the pooled prevalence of 14% (95% CI: 5-25%) of T. gondii infection in humans from southern African countries was lower than the seroprevalence reported from a metaanalysis conducted on pregnant women in African regions, American regions, eastern Mediterranean regions, Europe, the South-East Asia region, globally [85], and in some North African countries (Tunisia, Egypt, and Morocco) [66].…”

Section: Discussionmentioning

confidence: 99%

“…However, rarely, consumption of non-pasteurized milk or milk products can serve as a potential source of T. gondii transmission [2,5,6]. Oysters and mussels can act as reservoir hosts for infective oocysts, which can later be transmitted to other animals upon consumption [2,[7][8][9]. Parasites attain maturity in the intestine of felids and start releasing numerous oocysts into the environment within three to 18 days post-infection [10].…”

Section: Introductionmentioning

confidence: 99%

Toxoplasma gondii Infections in Animals and Humans in Southern Africa: A Systematic Review and Meta-Analysis

2022

View full text Add to dashboard Cite

Background: Toxoplasma gondii is an apicomplexan parasite with zoonotic importance worldwide especially in pregnant women and immunocompromised people. This study is set to review the literature on T. gondii infections in humans and animals in southern Africa. Methods: We extracted data regarding T. gondii infections from published articles from southern Africa from 1955 to 2020 from four databases, namely Google Scholar, PubMed, EBSCO Host, and Science Direct. Forty articles from eight southern African countries were found eligible for the study. Results: This review revealed a paucity of information on T. gondii infection in southern African countries, with an overall prevalence of 17% (95% CI: 7–29%). Domestic felids had a prevalence of 29% (95% CI: 7–54%), wild felids 79% (95% CI: 60–94), canids (domestic and wild) 69% (95% CI: 38–96%), cattle 20% (95% CI: 5–39%), pigs 13% (95% CI: 1–29%), small ruminants (goats and sheep) 11% (95% CI: 0–31%), chicken and birds 22% (95% CI: 0–84%), and humans 14% (95% CI: 5–25%). Enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT) constituted the most frequently used diagnostic tests for T. gondii. Conclusions: We recommend more focused studies be conducted on the epidemiology of T. gondii in the environment and human population, most especially the at-risk populations.

show abstract

“…and showing that the T. gondii oocyst stay infectious even after they pass through the marine invertebrate digestive system like in the oysters and mussels. On the other hand Coupe et al, (2019) (37) used a variety of PCR-based methods to confirm the presence of the Toxoplasma gondii parasite›s DNA in the green mussel Pern canaliculus using 2 Nested-PCR assays that target the dhps gene and B1 gene and 2 Real time-PCR assays that target B1-gene and the repeating element 529-bp By comparing the results of the two assays, it was concluded that the rep529 qPCR test may be preferred for future mussel studies, but the DNA sequence is required for final confirmation of the detection of T. gondii DNA. However, there remain no evidences about the human infection by the T. gondii as a result of the consumption of the raw meat of the sea animals or their products.…”

Section: Cool Et Al(2000)mentioning

confidence: 98%

Molecular Detection of Toxoplasma gondii in Helix pomatia in Wasit Province Iraq

Duaa Abdul Khaliq AL-Quraishy,

Altamemy A. K. Aakool

2021

Indian Journal of Forensic Medicine & Toxicology

View full text Add to dashboard Cite

DNA of a total of 96 samples of (32) enteric, (32) gastric and (32) pod of Helix pomatia has been obtained through the use of the Genomic DNA Extraction kit and tested by the use of the Real time PCR and specific set of the primers and probe for the detection of the Toxoplasma gondii B1 gene. Six enteric samples and only 2 gastric samples have been positive while the others have been negative, that result indicates the presence of the T. gondii in that snail species, and based upon those results infection can either be acquired from the contaminated environment or as a result of consuming the grass that has been contaminated with parasite oocysts. The negative result of pod samples may be due to the failure of filtration the infectious stages of parasites into the organs that close contacts with environment or inability of the parasites in surviving in those organs.

show abstract

Comparison of PCR assays to detect Toxoplasma gondii oocysts in green-lipped mussels (Perna canaliculus)

Cited by 12 publications

References 70 publications

Contamination of Soil, Water, Fresh Produce, and Bivalve Mollusks with Toxoplasma gondii Oocysts: A Systematic Review

Contamination of Soil, Water, Fresh Produce, and Bivalve Mollusks with Toxoplasma gondii Oocysts: A Systematic Review

Toxoplasma gondii Infections in Animals and Humans in Southern Africa: A Systematic Review and Meta-Analysis

Molecular Detection of Toxoplasma gondii in Helix pomatia in Wasit Province Iraq

Contact Info

Product

Resources

About