Comparison of PCR and ELISA methods for the detection of bovine leucosis in dried blood spots

Saushkin, N. Yu.; Samsonova, J.V.; Осипов, А. И.; Кондаков, С. Э.; Khammadov, Nail I.; Usoltsev, K.V.; Makaev, Kh. Z.; Chernov, A. N.

doi:10.3103/s0027131416050084

Cited by 7 publications

(3 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…That is why, along with other numerous approaches and devices for volumetric sampling of whole blood, the researchers consider the alternative non-cellulose materials for DBS preparations to minimize these effects. Some veterinary studies were performed with the use of nitrocellulose, nylon (Vilček et al 2001 ; Wang et al 2002 ) and fiberglass membranes (Samsonova et al 2014 , 2017 , 2019a , b ; Saushkin et al 2016a , b , 2019 ). Just a few works were devoted to compare cellulose and non-cellulose membrane carriers for particular investigations.…”

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

“…Later, Nobuto cellulose strips were found useful for wildlife sampling as a convenient tool for whole blood collection in a field, directly from a puncture or a wound (Table 1 ). Another type of stripes made of fiberglass and divided into equal zones for collecting and analysing of dried whole blood, serum or milk by ELISA and PCR were described (Samsonova et al 2014 , 2017 , 2019a , b ; Saushkin et al 2016a , b , 2019 ). The proposed strip provides volumetric biofluid microsampling due to the properties of absorbing non-cellulose material consisted from solid fibers and the shape of the strip.…”

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

“…Analysis of the published works revealed that to perform serological tests the conditions of dried biosample extraction from cellulose carrier were chosen by authors empirically and usually included incubation of dried sample in a buffer solution from 1–2 h to overnight or even 24 h at room temperature or + 4 °C. At the same time it was shown that 20–30 incubation under shaking at room temperature is enough to release antibodies from whole blood, serum or plasma dried on a glassfibre strip (Saushkin et al 2016b , a , 2019 ; Samsonova et al 2019a , b ). Some works described the optimization of antibodies elution time and temperature (Hopkins et al 1998 ; Chadio et al 2002 ).…”

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

See 2 more Smart Citations

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

2022

View full text Add to dashboard Cite

Dried Blood Spots (DBS) technology has become a valuable tool in medical studies, however, in veterinary and biological research DBS technology applications are still limited. Up-to-date no review has comprehensively integrated all the evidence existing across the fields, technologies and animal species. In this paper we summarize the current applications of DBS technology in the mentioned areas, and provide a scope of different types of dried sample carriers (cellulose and non-cellulose), sampling devices, applicable methods for analyte extraction and detection. Mammals, birds, insects and other species are represented as the study objects. Besides the blood, the review considers a variety of specimens, such as milk, saliva, tissue samples and others. The main applications of dried samples highlighted in the review include epidemiological surveys and monitoring for infections agents or specific antibodies for disease/vaccination control in households and wildlife. Besides the genetic investigations, the paper describes detection of environmental contaminants, pregnancy diagnosis and many other useful applications of animal dried samples. The paper also analyses dried sample stability and storage conditions for antibodies, viruses and other substances. Finally, recent developments and future research for DBS technology in veterinary medicine and biological sciences are discussed.

show abstract

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

See 1 more Smart Citation

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

2022

View full text Add to dashboard Cite

show abstract

Strip-dried whole milk sampling technique for progesterone detection in cows by ELISA

Samsonova

Осипов

Кондаков

2017

Talanta

View full text Add to dashboard Cite

Comparative research of effectiveness of cellulose and fiberglass porous membrane carriers for bio sampling in veterinary and food industry monitoring

Gusev

Vasyukova

Zakharova

et al. 2017

AIP Conference Proceedings

View full text Add to dashboard Cite

Abstract. The aim of proposed research is to study the applicability of fiberglass porous membrane materials in a new strip format for dried blood storage in food industry monitoring. A comparative analysis of cellulosic and fiberglass porous membrane materials was carried out to obtain dried samples of serum or blood and the possibility of further species-specific analysis. Blood samples of Sus scrofa were used to study the comparative effectiveness of cellulose and fiberglass porous membrane carriers for long-term biomaterial storage allowing for further DNA detection by real-time polymerase chain reaction (PCR) method. Scanning electron microscopy of various membranes -native and with blood samples -indicate a fundamental difference in the form of dried samples. Membranes based on cellulosic materials sorb the components of the biological fluid on the surface of the fibers of their structure, partially penetrating the cellulose fibers, while in the case of glass fiber membranes the components of the biological fluid dry out as films in the pores of the membrane between the structural filaments. This fundamental difference in the retention mechanisms affects the rate of dissolution of the components of dry samples and contributes to an increase in the efficiency of the desorption process of the sample before subsequent analysis. Detecting of pig DNA in every analyzed sample under the performed Real-time PCR as well as good state of the biomaterial preservation on the glass fiber membranes was clearly demonstrated. Good biomaterials preservation has been revealed on the test cards for 4 days as well as for 1 hour.

show abstract

Comparison of PCR and ELISA methods for the detection of bovine leucosis in dried blood spots

Cited by 7 publications

References 4 publications

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

Strip-dried whole milk sampling technique for progesterone detection in cows by ELISA

Comparative research of effectiveness of cellulose and fiberglass porous membrane carriers for bio sampling in veterinary and food industry monitoring

Contact Info

Product

Resources

About