Comparison of passive haemagglutination and haemagglutination-inhibition techniques for detection of antibodies to rubella virus.

Abstract

The HI technique used in this study has been described in detail elsewhere (Gust, 1973). Briefly, the test involved the treatment of sera with either kaolin or heparin-manganous chloride to remove nonspecific inhibitors, followed by adsorption with 50% pigeon erythrocytes. Doubling dilutions of the treated sera were incubated with 6 to 8 haemagglutinating doses of rubella antigen and 0-2 % pigeon erythrocytes.PHA TEST PHA antibodies were detected using a commercially available test kit (Rubacell, Abbott Laboratories, Chicago, Illinois). Stabilised human erythrocytes, sensitised with rubella-virus soluble antigen, were reacted with untreated human sera for determination of antibody levels. The exact procedure followed was according to the manufacturer's instructions except 128 on 11 May 2018 by guest. Protected by copyright.

Differences in antibody responses with rapid agglutination tests for the detection of rubella antibodies

“…Because of rubella immunization programs and a need to determine immunity early in pregnancy, sensitive but specific screening assays are required. Traditionally, hemagglutination inhibition (HI) tests were performed for this purpose (4,19), but more recently many laboratories have adopted other technologies, such as radioimmunoassay, enzyme immunoassay (EIA) (4,11), single radial hemolysis, passive hemagglutination (1,7,10), immunofluorescence (3), or latex agglutination (2,6,24). Many of these tests are available from commerical sources.…”