Comparison of partial malolactic enzyme gene sequences for phylogenetic analysis of some lactic acid bacteria species and relationships with the malic enzyme

Abstract

DNA sequences covering 36% of the mle gene that encodes the malolactic enzyme were determined for 13 strains of lactic acid bacteria, representing Pediococcus, Leuconostoc, Lactobacillus and Oenococcus genera. The sequences were aligned with the corresponding region of mleS in Lactococcus lactis. The phylogenetic distance matrix tree of all mle sequences was compared with the 165 rRNA phylogenetic tree. The analysis showed that the mle fragment evolved more rapidly than the 16s gene and differently. Pediococcus and Lactobacillus species were intermixed in the 165 rRNA tree whereas they were separated in the mle tree. Leuconostoc mesenteroides and Oenococcus oeni were distinct from other species in the 16s rRNA tree, whereas they were intermixed with Lactobacillus species and Lactococcus lactis in the mle tree.The amino acid sequences deduced from partial mle genes were aligned with 22 malic enzyme sequences and the corresponding phylogenetic tree was constructed. Malic and malolactic enzymes were distinct a t the phylogenetic level, except for malic enzymes of yeast and Escherichia coli which were nearer the malolactic enzymes than the other malic enzymes. The analysis of conserved sites showed several interesting amino acids specific to either malic enzyme or malolactic enzyme.

Genomic subpopulations within the species Pediococcus acidilactici detected by multilocus typing analysis: relationships between pediocin AcH/PA-1 producing and non-producing strains The GenBank accession numbers for the sequences determined in this work are given in Table 4 T4 .

“…AluI digestion did not permit discrimination among the strains tested, while RsaI and TaqI highlighted three different mle alleles : one characteristic of strains DSMZ 20284 T , DSMZ 20238, ATCC 8042, ATCC 12697 and ATCC 25740, one charcteristic of pediocin-producing strains and of PG and Pdi11 strains, and another typical of all chili bo isolates. The sizes of the restriction fragments obtained were in agreement with the theoretical analysis of the sequence published (Groisillier & Lonvaud-Funel, 1999) only for the mlef fragments amplified from the group of strains obtained from DSMZ and ATCC (Fig. 2c, Table 3).…”

Cloning and Sequencing of the Histidine Decarboxylase Genes of Gram-Negative, Histamine-Producing Bacteria and Their Application in Detection and Identification of These Organisms in Fish

“…Several authors have suggested that there is no correlation between 16S rRNA gene and protein-coding genes when the latter is more dependent on selection pressure (17) or had been horizontally transferred across groups at an earlier stage of divergence (7). However, we could not find evidence of the horizontal transfer of this gene based on the branching order of hdc in this study.…”

The Biochemistry of Malic Acid Metabolism by Wine Yeasts – A Review

“…The S. pombe malate transporter is unable to transport fumarate, although fumarate inhibits the uptake of L-malate (Saayman et al, 2000). The cytosolic NAD-dependent malic enzyme (EC 1.1.1.38) has a high substrate affinity (Km = 3.2) (Maconi et al, 1984) and sequence analysis of mae2p revealed a strong phylogenetic link with other malic and malolactic enzymes (Viljoen et al, 1994;Groisillier and Lonvaud-Funel, 1999). …”