Comparison of oxidative stress biomarkers in renal tissues of d-galactose induced, naturally aged and young rats

Aydın, Seval; Yanar, Karolin; Atukeren, Pınar; Dalo, Enis; Sitar, Mustafa Erinç; Uslu, Ezel; Caf, Nazlı; Çakatay, Ufuk

doi:10.1007/s10522-011-9370-3

Cited by 51 publications

(39 citation statements)

References 50 publications

(48 reference statements)

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“…liver and blood cells (Lei et al, 2008;Xie and Ling, 2008;Aydin et al, 2012;Hsia et al, 2012;Wu et al, 2012) more recently, we found phenotypes of Polycystic Ovary Syndrome (PCOS) in this model (Park and Choi, 2012). These induced phenotypes may be the result of excessive formation of Reactive Oxygen Species (ROS) and D-gal-induced accumulation of AGEs (Song et al, 1999).…”

Section: Ajimentioning

confidence: 79%

Production of Cross-Reactive Autoantibody Binding to Bovine Serum Albumin in the D-Galactose-Induced Aging Mouse Model

Park¹

2014

American Journal of Immunology

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The D-galactose (D-gal)-induced animal model, generated by repeated subcutaneous D-gal injections over approximately 6 weeks, has been frequently used for diabetes and aging research. However, little research has investigated the direct correlation between D-gal and autoantibody formation despite several reports on diabetes-and aging-related autoantibodies. The purpose of this study was to determine whether repetitive injection of D-gal can induce autoantibody production in mice. First, we used Bovine Serum Albumin (BSA) and Advanced Glycation End products (AGE)-BSA as the test antigens. The immunoreactivity of serum samples from mice treated with D-gal for 6 weeks was evaluated using Enzyme-Linked Immunosorbent Assay (ELISA). We found that serum samples of D-gal-treated mice had significantly high antibody titers against both BSA and AGE-BSA. Furthermore, the result showed that aminoguanidine treatment, an AGE inhibitor tended to decrease this immunoreactivity. The results of competitive inhibition ELISA using BSA and AGE-BSA as the competitors suggested that the serum samples from D-gal-treated mice contained antibodies not only against BSA but also specific to AGE-BSA. To assess whether the immunoreactivity against BSA is comparable to that against Mouse Serum Albumin (MSA), we examined the reactivity of D-gal-induced antibodies against MSA. Unexpectedly, D-gal-induced antibodies did not react with MSA. This suggests that the production of antibodies by Dgal is in response to an unknown antigen(s), aside from MSA, in mice and that this unknown antigen(s) may share similar sequences or three-dimensional structures with BSA.

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Section: Ajimentioning

confidence: 79%

Production of Cross-Reactive Autoantibody Binding to Bovine Serum Albumin in the D-Galactose-Induced Aging Mouse Model

Park¹

2014

American Journal of Immunology

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“…Recently, studies focused on the roles of galactose in cellular senescence have shown that the mechanisms underlying d -gal -induced aging likely involve glucose and 1ipid metabolic disorders, increased oxidative damage, the accumulation of advanced glycation end products, impaired elimination of abnormal substances, accelerated cell apoptosis, and enhanced insulin resistance 22,24. The effects produced by d -gal differ with dosage (varying between 50 and 1,250 mg/kg/d), age group (varying between 6 weeks and 6 months), and time interval (varying between 6 and 10 weeks) 12. According to the literature,14,15 we induced aging in 12-weeks-old rats using two different d -gal doses (125 mg/kg/d and 500 mg/kg/d).…”

Section: Discussionmentioning

confidence: 99%

Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats

Liu¹,

Hu²,

Mao³

et al. 2017

CIA

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BackgroundRecently, the d-galactose (d-gal)-induced mimetic aging rat model has been widely used in studies of age-associated diseases, which have shown that chronic d-gal exposure induces premature aging similar to natural aging in rats. With the increasing rate of sepsis in the geriatric population, an easy-access animal model for preclinical studies of elderly sepsis is urgently needed. This study investigates whether a sepsis model that is established in d-gal-induced aging rats can serve as a suitable model for preclinical studies of elderly patients with sepsis.ObjectiveTo investigate the acute kidney injury (AKI) and inflammatory response of sepsis following cecal ligation and puncture (CLP) in d-gal-induced aging rats.MethodsTwelve-week-old male Sprague Dawley rats were divided into low-dose d-gal (L d-gal, 125 mg/kg/d), high-dose d-gal (H d-gal, 500 mg/kg/d), and control groups. After daily subcutaneous injection of d-gal for 6 weeks, the CLP method was used to establish a sepsis model.ResultsThe mortality was 73.3%, 40%, and 33.3% in the H d-gal, L d-gal, and control groups, respectively. Blood urea nitrogen, creatinine, plasma neutrophil gelatinase-associated lipocalin, interleukin-6, interleukin-10, and tumor necrosis factor-α were markedly increased in the H d-gal group after establishment of the sepsis model (H d-gal vs control, P<0.05 at 12 h and 24 h post-CLP). The rate of severe AKI (RIFLE-F) at 24 h post-CLP was 43% for both the control and L d-gal groups and 80% for the H d-gal group.ConclusionHigh-dose- d-gal-induced aging rats are more likely to die from sepsis than are young rats, and probably this is associated with increased severity of septic AKI and an increased inflammatory response. Therefore, use of the high-dose- d-gal-induced aging rat model of sepsis for preclinical studies can provide more useful information for the treatment of sepsis in elderly patients.

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“…In addition to the aforementioned pathologic states, tissue factors such as protein, deoxyribonucleic acid, and lipid oxidation products are associated with renal aging 3,4…”

Section: Introductionmentioning

confidence: 99%

Oxidative damage parameters in renal tissues of aged and young rats based on gender

Uzun¹,

Korkmaz²,

Sitar³

et al. 2013

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PurposeAging is characterized by a gradual functional decrease of all systems including the kidneys. Growing evidence links altered lipid protein redox-homeostasis with renal dysfunction. The effect of sexual dimorphism on the lipid protein redox-homeostasis mechanisms in the aging kidney is obscure. In the current study, we aimed to investigate redox homeostasis as it related to sexual dimorphism on protein oxidation and lipid peroxidation parameters, as protein carbonyl (PCO), total thiol (T-SH), advanced oxidation protein products (AOPP), malondialdehyde, glutathione (GSH), and superoxide dismutase (SOD) activity, as potential aging biomarkers, which may contribute to an analysis of the free radical theory of aging.Materials and methodsThe study was carried out with 16 naturally aged rats (24 months old; eight males and eight females) and their corresponding young rat groups as controls (6 months old; eight males and eight females). All of the aforementioned parameters (PCO, T-SH, AOPP, MDA, GSH, SOD) were measured manually instead of automated devices or ELISA kits.ResultsPCO, AOPP, and malondialdehyde levels in aged rats were significantly higher in the older rat group than in the younger rat group, whereas SOD activities were significantly lower in old rats. T-SH levels were not significantly different in male groups; however, T-SH levels were lower in the aged female group than in the young female control group. In addition, GSH levels were significantly different between the aged rat group and the corresponding young control group for both genders.ConclusionWith respect to PCO and AOPP, impaired redox homeostasis is substantially more prominent in males than females. The decrease of G-SH levels in male groups could be attributed to stabilizing the redox status of protein thiol groups by the depletion of the GSH groups. Considering the results, the renal tissue proteins and lipids in different genders may have different susceptibilities to oxidative damage.

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Comparison of oxidative stress biomarkers in renal tissues of d-galactose induced, naturally aged and young rats

Cited by 51 publications

References 50 publications

Production of Cross-Reactive Autoantibody Binding to Bovine Serum Albumin in the D-Galactose-Induced Aging Mouse Model

Production of Cross-Reactive Autoantibody Binding to Bovine Serum Albumin in the D-Galactose-Induced Aging Mouse Model

Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats

Oxidative damage parameters in renal tissues of aged and young rats based on gender

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