Comparison of Oxford Agar, PALCAM and Listeria monocytogenes Blood Agar for the recovery of L. monocytogenes from foods and environmental samples

Pinto, M.; Burri, Solange; Mena, Cristina; Almeida, Gonçalo; Carneiro, Luísa; Teixeira, Paula; Gibbs, Paul

doi:10.1016/s0956-7135(01)00052-4

Cited by 22 publications

(9 citation statements)

References 12 publications

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“…These results are in accordance with the results reported by other authors (Gouws and Liedemann 2005;Furrer et al 1991) showing a high frequency of Listeria species isolation, mainly L. innocua, from Oxford and PALCAM agar. This has been witnessed in comparative studies using alternative isolation media (Vlaemynck et al 2000;Karpísková et al 2000;Greenwood et al 2005;Pinto et al 2001) and has led to the introduction of improved selective-differential isolation media, such as ALOA (Vlaemynck et al 2000), in updated standard isolation procedures (Anon 2005b), thus enhancing the detection ratio for L. monocytogenes. The isolates under study were recovered between 1999 and 2002, and following the regulations in force at that time, Oxford and PALCAM were used as selective media instead of ALOA, which explains the high recovery of other Listeria spp.…”

Section: Discussionmentioning

confidence: 99%

Reliability of Listeria monocytogenes Identification by Specific PCR Assessed by Phenotypic and Genotypic Techniques

Aznar

Elizaquível

2008

Food Anal. Methods

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This study evaluates the possibility of using polymerase chain reaction (PCR) for rapid identification of food-borne Listeria monocytogenes as an alternative to API Listeria system and estimates the incidence of API Listeria misidentifications in food-borne Listeria species. A total of 198 strains, 11 L. monocytogenes, 28 other Listeria species, and 159 food isolates were phenotypically and genotypically characterized by API Listeria profiles and randomly amplified polymorphic DNA (RAPD) profiles, respectively. They were also tested for PCR amplification using genusand species-specific primers. Clustering analysis of phenotypic and genotypic data showed discrepancies in species identification of some isolates by API Listeria profiles. Their identities were confirmed by 16S rDNA sequencing, and thus, it was revealed that 33% of Listeria innocua and 19% of Listeria welshimeri were misidentified as L. monocytogenes by API Listeria profiles. Reliable identification of L. monocytogenes was obtained by LM1-LM2 specific primers which allowed PCR amplification only in reference strains and isolates previously identified as L. monocytogenes by RAPD and 16S rDNA sequence analysis. These results corroborate the suitability of specific PCR as a rapid and accurate test for the identification of L. monocytogenes, avoiding misidentification with other Listeria species commonly found in food products.

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Section: Discussionmentioning

confidence: 99%

Reliability of Listeria monocytogenes Identification by Specific PCR Assessed by Phenotypic and Genotypic Techniques

Aznar

Elizaquível

2008

Food Anal. Methods

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“…Oxford agar was initially developed by Curtis et al (1989) for the isolation of L. monocytogenes from clinical specimens. Oxford agar has been extensively used in many studies for the isolation and detection of L. monocytogenes from various food samples ( Pinto et al, 2001 ; Rudol and Scherer, 2001 ; Gudbjörnsdóttir et al, 2004 ; Mena et al, 2004 ; Alessandria et al, 2010 ). Oxford consists of Columbia Blood Agar with 23 g/L protease peptones, 5.0 g/L sodium chloride, and 1.0 g/L starch.…”

Section: Detection and Identification Of Listeria Monocytogmentioning

confidence: 99%

An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food

et al. 2015

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Listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. Hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. This review provides insight into the methods for cultural detection, enumeration, and molecular identification of L. monocytogenes in various food samples. There are a number of enrichment and plating media that can be used for the isolation of L. monocytogenes from food samples. Enrichment media such as buffered Listeria enrichment broth, Fraser broth, and University of Vermont Medium (UVM) Listeria enrichment broth are recommended by regulatory agencies such as Food and Drug Administration-bacteriological and analytical method (FDA-BAM), US Department of Agriculture-Food and Safety (USDA-FSIS), and International Organization for Standardization (ISO). Many plating media are available for the isolation of L. monocytogenes, for instance, polymyxin acriflavin lithium-chloride ceftazidime aesculin mannitol, Oxford, and other chromogenic media. Besides, reference methods like FDA-BAM, ISO 11290 method, and USDA-FSIS method are usually applied for the cultural detection or enumeration of L. monocytogenes. most probable number technique is applied for the enumeration of L. monocytogenes in the case of low level contamination. Molecular methods including polymerase chain reaction, multiplex polymerase chain reaction, real-time/quantitative polymerase chain reaction, nucleic acid sequence-based amplification, loop-mediated isothermal amplification, DNA microarray, and next generation sequencing technology for the detection and identification of L. monocytogenes are discussed in this review. Overall, molecular methods are rapid, sensitive, specific, time- and labor-saving. In future, there are chances for the development of new techniques for the detection and identification of foodborne with improved features.

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“…Pinto et al (2001) for example, in a comparative assessment of food and environmental samples, found higher sensitivity for the recovery of L. monocytogenes using Listeria monocytogenes Blood agar (LMBA) compared with both PALCAM and Oxford agars. These authors were unable to distinguish any difference in results using the selective media recommended by the ISO.…”

Section: Resultsmentioning

confidence: 99%

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Benetti

Monteiro

Beux

et al. 2012

Braz. J. Pharm. Sci.

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The aim of this study was to determine the prevalence of Listeria sp. in refrigerated sausages, and to compare the performance of the selective plating media employed in the ISO 11290-1 method (PALCAM and Oxford agars) with chromogenic agars (Chromogenic Listeria agars CM 1080 (OCLA) and CM 1084). The prevalence of Listeria sp. detected was 52.9%, comprising 13.7% L. monocytogenes strains. The efficacy of the four agars for the isolation of L. monocytogenes proved to be satisfactory. Despite differences in composition of the chromogenic media assessed, these disparities did not affect concordance among results. However, PALCAM agar was shown to suppress other microorganisms more effectively, being more applicable for detecting Listeria strains present in lower quantities. Based on these results, the use of PALCAM agar, in combination with a chromogenic media, is recommended for enhanced isolation of atypical Listeria sp. strains in meat products.Uniterms: Listeria sp./detection/meat products. Meat products/qualitative analysis. Refrigerated sausages/ qualitative analysis. PALCAM Agar. Oxford Agar. Chromogenic Agar.Este estudo teve como objetivo a análise da prevalência de Listeria sp. em linguiças resfriadas e a comparação dos meios seletivos utilizados no plaqueamento do método ISO 11290-1 (Ágar PALCAM e Ágar Oxford), e ágares cromogênicos (Ágares Listeria Cromogênico CM 1080 (OCLA) e CM 1084 (ISO)). A frequência de Listeria sp. foi de 52,9%, sendo que destas, 13,7% corresponderam à L. monocytogenes. A eficácia dos quatro ágares para o isolamento de L. monocytogenes demonstrou-se satisfatória. Apesar de haver algumas diferenças nas composições dos meios cromogênicos analisados, estas não pareceram influenciar nas concordâncias entre os resultados expressos. Contudo, o ágar PALCAM mostrou-se mais eficaz na supressão de outros micro-organismos, aumentando, assim, a possibilidade de detecção de espécies de Listeria presentes em número reduzido. Através deste trabalho sugere-se a utilização do ágar PALCAM associado a um meio cromogênico para aumentar a chance de isolamento de cepas atípicas de Listeria sp. em produtos cárneos.Unitermos: Listeria sp./detecção/produtos cárneos. Produtos cárneos/análise qualitativa. Lingüiças resfriadas/análise qualitativa. Ágar PALCAM. Ágar Oxford. Ágar cromogênico.

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Comparison of Oxford Agar, PALCAM and Listeria monocytogenes Blood Agar for the recovery of L. monocytogenes from foods and environmental samples

Cited by 22 publications

References 12 publications

Reliability of Listeria monocytogenes Identification by Specific PCR Assessed by Phenotypic and Genotypic Techniques

Reliability of Listeria monocytogenes Identification by Specific PCR Assessed by Phenotypic and Genotypic Techniques

An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

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