Comparison of organic matter degradation and microbial community during thermophilic composting of two different types of anaerobic sludge

Nakasaki, Kiyohiko; Tran, Le Thi Hong; Idemoto, Yoshito; Abe, Michiharu; Rollon, Analiza P.

doi:10.1016/j.biortech.2008.07.046

Cited by 134 publications

(44 citation statements)

References 34 publications

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“…This could be related to the richness of lignocellulose substrate in mixture A. Pérez et al (2002) and Nakasaki et al (2009) showed that actinobacteria produce an active enzyme to degrade lignocellulotic compounds. Tuomela et al (2000); Steger et al (2007) and Xiao et al (2011) have shown that actinomycetes are activated later and contribute to the degradation of recalcitrant compounds and the formation of the stable compounds of ''humic substance''.…”

Section: Enumeration Of Mesophilic and Thermophilic Indigenous Microfmentioning

confidence: 99%

Use of the co-composting time extract agar to evaluate the microbial community changes during the co-composting of activated sludge and date palm waste

Fels

Ouhdouch

Hafidi

2015

Int J Recycl Org Waste Agricult

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Section: Enumeration Of Mesophilic and Thermophilic Indigenous Microfmentioning

confidence: 99%

Use of the co-composting time extract agar to evaluate the microbial community changes during the co-composting of activated sludge and date palm waste

Fels

Ouhdouch

Hafidi

2015

Int J Recycl Org Waste Agricult

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“…Alternatively, anaerobic bacteria, such as Clostridium thermocellum, can also be used for biomass conversion. Recent studies have shown that certain thermophilic anaerobes, such as C. thermocellum strains, have sufficiently high growth and metabolic rates when grown on cellulose to make them competitive with fungi (14,32,34,36,45,55). Many of these anaerobic bacteria utilize high-affinity cellulases organized in stable, membrane-bound, multienzyme cellulosomes, which greatly improve the efficiency of biomass conversion compared to fungi (46,51).…”

mentioning

confidence: 99%

Isolation and Characterization of Shigella flexneri G3, Capable of Effective Cellulosic Saccharification under Mesophilic Conditions

Wang

Gao

Ren

et al. 2011

Appl Environ Microbiol

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A novel Shigella strain (Shigella flexneri G3) showing high cellulolytic activity under mesophilic, anaerobic conditions was isolated and characterized. The bacterium is Gram negative, short rod shaped, and nonmotile and displays effective production of glucose, cellobiose, and other oligosaccharides from cellulose (Avicel PH-101) under optimal conditions (40°C and pH 6.5). Approximately 75% of the cellulose was hydrolyzed in modified ATCC 1191 medium containing 0.3% cellulose, and the oligosaccharide production yield and specific production rate reached 375 mg g Avicel ؊1 and 6.25 mg g Avicel ؊1 h ؊1 , respectively, after a 60-hour incubation. To our knowledge, this represents the highest oligosaccharide yield and specific rate from cellulose for mesophilic bacterial monocultures reported so far. The results demonstrate that S. flexneri G3 is capable of rapid conversion of cellulose to oligosaccharides, with potential biofuel applications under mesophilic conditions.Lignocellulosic biomass is abundant in nature, as well as in agricultural, forestry, and municipal wastes, and can be used as an excellent bioconversion feedstock. Biomass-derived saccharides, such as glucose, cellobiose, and other minor sugars, can be readily fermented by appropriate microbes into bioenergy products, such as hydrogen, ethanol, biodiesel, and other commodity chemicals. However, the high cost of converting biomass to sugars is the primary factor impeding establishment of a cellulosic-biofuels industry (24, 40). Lignocellulosic biofuels can be competitive on an industrial scale if efficient technologies can be developed (29,39). Currently, the most efficient process for utilization of cellulose as a feed stock is either a three-step process (separate hydrolysis and fermentation [SHF]) involving separate pretreatment, cellulose hydrolysis (i.e., saccharification), and hexose and pentose fermentation steps or a two-step process (simultaneous saccharification and fermentation [SSF]) involving separate pretreatment and simultaneous saccharification of hexose and pentose fermentation (48, 64). Combining hydrolysis of cellulose with simultaneous fermentation of hexose and pentose in a single process, i.e., direct microbial conversion (DMC), is an ideal strategy for converting cellulosic biomass to ethanol. However, no single microorganism/community can implement DMC with high efficiency (67). In any of these configurations, rapid and efficient saccharification is critical for developing competitive biotechnologies for cellulosic-biofuel production.In nature, cellulose is hydrolyzed to oligosaccharides by microorganisms, mainly fungi (e.g., brown-, white-, and soft-rot fungi) and bacteria (e.g., Clostridium and Cellulomonas), which produce either free cellulolytic enzymes or extracellular enzyme complexes known as cellulosomes (12). Many white-rot Basidiomycetes and some Actinomycetes have been employed for hydrolyzing lignocellulosic materials. For example, Trichoderma reesei has shown the highest cellulolytic activity currently known (27)....

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“…The dominant genus in the thermophilic stage of sludge bio-drying was Ureibacillus (maximum, 8.6 %). Nakasaki et al (2009) compared the microbial community during thermophilic composting of two different types of anaerobic sludge using PCR-DGGE, and Ureibacillus sp. was the observed dominant band during the composting of S-sludge (a high concentration of proteins).…”

Section: Evolution Of Dom In Sludge Bio-dryingmentioning

confidence: 99%

“…Both consist of mesophilic and thermophilic stages involving numerous microorganisms, but the former aims at MC removal, while the latter focuses on biostabilization and the maturity of the composted materials (Zhang et al 2009). Overviews of the evolution of the microbial community during sludge composting, including group succession and utilizable substrate for different process stages and temperature ranges, have been reported previously (Nakasaki et al 2009). Also, evolution of DOM during composting has been widely studied using Fourier transform infrared spectroscopy (FTIR) and three-dimensional excitation-emission matrix (EEM) methods (Said-Pullicino and Gigliotti 2007;Straathof and Comans 2015).…”

Section: Introductionmentioning

confidence: 99%

Effects of aeration strategy on the evolution of dissolved organic matter (DOM) and microbial community structure during sludge bio-drying

Zhang

Cai

et al. 2015

Appl Microbiol Biotechnol

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Sludge bio-drying in which sludge is dried by means of the heat generated by the aerobic degradation of its own organic substances has been widely used for sludge treatment. A better understanding of the evolution of dissolved organic matter (DOM) and its degradation drivers during sludge bio-drying could facilitate its control. Aeration is one of the key factors that affect sludge bio-drying performance. In this study, two aeration strategies (pile I-the optimized and pile II-the current) were established to investigate their impacts on the evolution of DOM and the microbial community in a full-scale sludge bio-drying plant. A higher pile temperature in pile I caused pile I to enter the DOM and microbiology stable stage approximately2 days earlier than pile II. The degradation of easily degradable components in the DOM primarily occurred in the thermophilic phase; after that degradation, the DOM components changed a little. Along with the evolution of the DOM, its main degradation driver, the microbial community, changed considerably. Phyla Firmicutes and Proteobacteria were dominant in the thermophilic stage, and genus Ureibacillus, which was the primary thermophilic bacteria, was closely associated with the degradation of the DOM. In the mesophilic stage, the microbial community changed significantly at first and subsequently stabilized, and the genus Parapedobacter, which belongs to Bacteriodetes, became dominant. This study elucidates the interplay between the DOM and microbial community during sludge bio-drying.

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Comparison of organic matter degradation and microbial community during thermophilic composting of two different types of anaerobic sludge

Cited by 134 publications

References 34 publications

Use of the co-composting time extract agar to evaluate the microbial community changes during the co-composting of activated sludge and date palm waste

Use of the co-composting time extract agar to evaluate the microbial community changes during the co-composting of activated sludge and date palm waste

Isolation and Characterization of Shigella flexneri G3, Capable of Effective Cellulosic Saccharification under Mesophilic Conditions

Effects of aeration strategy on the evolution of dissolved organic matter (DOM) and microbial community structure during sludge bio-drying

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