Comparison of Optimization Based on Information Theory with Optimization of Rs in Column Chromatography

Abstract

Optimization based on the function of mutual information (FUMI) is reviewed by comparison with optimization of Rs in column chromatography. The following variables are considered: mobile phase composition; pH; column length; the amount of internal standard; detection wavelength. FUMI is equivalent to the precision of measurements and takes a maximum at the optimal condition. However, Rs neither takes a maximum nor corresponds to the precision. This paper demonstrates that FUMI is a superior measure of chromatographic performance. 1765 Copyrght 0 1990 by Marcel Dekker, Inc.