Comparison of oocyte activation and Ca2+ oscillation-inducing abilities of round/elongated spermatids of mouse, hamster, rat, rabbit and human assessed by mouse oocyte activation assay

Abstract

Oocyte activation and Ca2+ oscillation-inducing abilities of round spermatid (ROS) and elongated spermatid (ELS) of some rodents and human were assessed by their injection into mouse (B6D2F1) oocytes (mouse test). With mice (B6D2F1, ICR) and rat, ROS displayed no oocyte activation or Ca2+ oscillation-inducing abilities. Although ELS could induce activation at 87, 86 and 31% of injected oocytes respectively, most of the intracellular calcium concentration ([Ca2+]i) responses of ELS-injected oocytes did not show oscillation patterns; only several transient [Ca2+]i rises (transient pattern) were seen. Similarly, with hamster, rabbit and human, while ROS could induce oocyte activation efficiently (70, 71 and 52% respectively), most of the [Ca2+]i patterns of injected oocytes were transient patterns, and not oscillation patterns. When ROS nuclei only from these latter species were injected into mouse oocytes, most of the oocytes could not be activated. [Ca2+]i patterns of oocytes injected with immature sperm cells changed from transient pattern to oscillation pattern while the cells were maturing into spermatozoa. With hamster ROS, oocyte-activating factor was found to be distributed mainly in the cytoplasm. It was interesting that there is a dissociation between the timings of appearance of oocyte activation and that of Ca2+ oscillation of oocytes injected with developing immature sperm cells.

Developmental competence in vivo and in vitro of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection with fresh or frozen-thawed spermatozoa

“…The sperm-borne oocyte-activation factor is not species-specific: pig cytosolic sperm factor can activate mouse and bovine oocytes (Fissore et al, 1998;Perry et al, 1999). In addition, spermatozoa from species, such as hamsters, rats, rabbits, humans or sea urchins can activate mouse oocytes (Rybouchikin et al, 1995;Wakayama et al, 1997;Yazawa et al, 2000). In other reports, interspecies fertilization after ICSI has been reported in conjunction with additional activation treatments.…”

The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis

“…1b) is the common response recorded after injection of mouse oocytes with different stages of human, mouse, hamster, rat and rabbit spermatogenic cells [34,35]. On the other hand, pattern-A oscillation (Fig.…”

“…Since the stability of sperm function after freeze-drying and long-term storage has not been fully investigated, the first experiment of the present study was designed to examine the ability of FD bull spermatozoa to induce calcium oscillations using interspecies ICSI assay. The interspecies ICSI assay using hybrid mouse oocytes, that was originally developed by Rybouchkin et al [34], has been used to investigate the SOAF activity of different spermatogenic cells in hamster, rat, rabbit, human [35] whale [29] and cynomolgus monkey [36] and chemically membrane-damaged spermatozoa in cattle, pig and human [37]. The advantages of using B6D2F1 mouse oocytes are their abilities to be loaded easily with calcium-sensitive fluorescent dye and their resistance to sham-injection treatment in term of oocyte activation.…”

ICSI et signalisation calcique

“…Le décours des oscillations Ca i varie en fonction de la maturité de chacun des gamètes utilisés lors de l'ICSI. Les techniques d'ELSI (elongated spermatid injection) et de ROSI (round spermatid injection) conduisent à l'activation de l'ovocyte chez la souris, le hamster et l'homme, l'activation de l'ovocyte après ROSI pouvant se produire sans l'apparition d'oscillations Ca i normales [5]. L'ICSI, pratiquée sur des ovocytes maturés in vitro, peut conduire également à des naissances [6].…”