Comparison of Oct4, Sox2 and Nanog Expression in Pancreatic Cancer Cell Lines and Human Pancreatic Tumor

Abstract

Background: Genes are involved in the control of stem cell self-renewal as a new class of molecular markers of cancer. Objectives: In this study, the expression of Oct4, Nanog and Sox2 in cell lines MIA Paca-2, PA-TU-8902 and AsPC-1 and pancreatic cancer tissue were examined. Materials and Methods: In this experimental study, cell lines, MIA Paca-2, PA-TU-8902 and AsPC-1, were cultured in DMEM (Dulbecco's Modified Eagles Medium) and RPMI-1640 (Roswell Park Memorial Institute) containing FBS 10% (fetal bovine serum) in a 37°C incubator containing Co 2 5% and humidity 90%. Samples of tumor and non-cancer pancreatic tumor were purchased Iran tumor bank. Extraction of RNA and synthesis of cDNA was performed. Expression levels of Oct4, Nanog and Sox2 were determined using Real-time PCR. The protein expression levels of target genes in the cell lines were studied by flow cytometry and immunocytochemistry. Results: The expression rate of Oct4, Nanog and Sox2 is more in the cancer cell lines than those in the control (normal tissue) samples. The protein expression levels of target genes in the cell lines were confirmed by flow cytometry and immunocytochemistry. Conclusions:The genes are involved in stem cell self-renewal as a new class of molecular markers of cancer that detected in the pancreatic cell lines. Maybe, these genes play important role in the uncontrolled proliferation of cancer cells.

SOX2 and Bcl-2 as a Novel Prognostic Value in Hepatocellular Carcinoma Progression

“…We found SOX2 mRNA expression in HCC tissue samples more than two folds higher than the normal tissue samples. Our results were consistent with studies showing SOX2 overexpression in gastric cancer [22], lung squamous cell carcinoma [23], gastrointestinal cancers [12], cervical cancer [24], gliomas [25], and pancreatic carcinoma [3,26].…”

“…The five-year survival rate of HCC is about 7% and the annual mortality is approximately one million worldwide. Most HCC-related deaths might be due to inappropriate early diagnosis, lack of effective treatment [2], or extremely aggressive and metastatic phenotypes of the tumor [3]. Although conventional therapies, such as surgical resection, chemotherapy, radiotherapy, and multimodality therapies improve the survival rate of HCC patients, many cases encounter recurrence, intrahepatic spread, and extrahepatic metastasis [4].…”

“…SOX2, an intron-less gene on the long (q) arm of chromosome 3, is the main member of the SRY-related HMG-box (SOX) gene family [3]. The products of these genes are transcription factors that bind to DNA via their HMG domain [3,11].…”

“…SOX2, an intron-less gene on the long (q) arm of chromosome 3, is the main member of the SRY-related HMG-box (SOX) gene family [3]. The products of these genes are transcription factors that bind to DNA via their HMG domain [3,11]. SOX2 plays an eminent role in organ development, morphogenesis, and specification of cell type in the embryonic period via cell proliferation, regulation of self-renewal, and maintaining pluripotency [3,8,12].…”

“…The products of these genes are transcription factors that bind to DNA via their HMG domain [3,11]. SOX2 plays an eminent role in organ development, morphogenesis, and specification of cell type in the embryonic period via cell proliferation, regulation of self-renewal, and maintaining pluripotency [3,8,12]. In normal adults, SOX2 expression is limited to somatic stem cells of the brain, stomach, and colon [8] via regulating neurogenesis in the developing fetal nervous system [12].…”