Comparison of nucleic acid and protein immunization for induction of antibodies specific for HIV-1 gp120

Peet, Nicky M.; McKeating, Jane A.; Ramos, Belén; Klonisch, Thomas; Souza, J. Brian de; Delves, Peter J.; Lund, Torben

doi:10.1046/j.1365-2249.1997.4411339.x

Cited by 23 publications

(12 citation statements)

References 31 publications

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“…We observed that mice from the three strains tested (BALB/c, CBA/J, and C57BL/6) seroconverted, although an heterogeneous response was observed among mice of the same strain; and this variability was observed in repeated immunization experiments. Variations in antibody responses were also observed by other authors in inbred strains (Homan et al 1994;Haddad et al 1997;He et al 1997;Peet et al 1997;Moynier et al 1998) and they have been attributed to dierential transfection eciencies in individual mice (Haddad et al 1997). Variability in antibody levels were also observed in ts-4-vaccinated mice.…”

Section: Discussionsupporting

confidence: 64%

Genetic immunization with plasmid DNA coding for the ROP2 protein of Toxoplasma gondii

Leyva¹,

Hérión²,

Saavedra³

2001

Parasitology Research

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The ROP2 protein of Toxoplasma gondii has previously been proposed as a vaccine candidate against toxoplasmosis. In this work we characterize the immune response induced by injection of plasmid DNA coding for this protein in three strains of mice (BALB/c, C57BL/6, and CBA/J) displaying different levels of susceptibility to toxoplasmosis and compare it with that obtained by vaccination with the live attenuated ts-4 strain of T. gondii. The ROP2 gene was cloned in the eukaryotic expression vector pcDNA3 and the resulting plasmid, named pcDNA3/ROP2, was used to immunize mice. After three immunizations with the plasmid, mice developed antibodies that could be detected by ELISA using a recombinant truncated form of ROP2; and these antibodies also recognized the natural protein by Western blot. Plasmid immunization generated antibodies against the ROP2 of both of the IgG1 and IgG2a isotypes in CBA/J and BALB/c mice and both of the IgG1 and IgG2c isotypes in C57BL/6 mice. However, animals vaccinated with the ts-4 strain generated only IgG2a (in CBA/J and BALB/c mice) or IgG2c (in C57BL/6 mice) against ROP2. Kinetic studies of the generation of isotypes indicated that both isotypes were generated at the same time. Mice immunized with the plasmid DNA did not resist a challenge with the virulent RH strain of T. gondii, while mice vaccinated with the ts-4 strain resisted the same challenge. However, in pcDNA3/ROP2-immunized BALB/c mice, death was significantly delayed with respect to the pcDNA3-immunized control group. These results suggest that plasmid immunization using the ROP2 gene generates a mixed T(H1)/T(H2) response against ROP2, which is different from that obtained by vaccination with live tachyzoites of the ts-4 strain (T(H1) response) and is not protective against the highly virulent RH strain of the parasite.

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Section: Discussionsupporting

confidence: 64%

Genetic immunization with plasmid DNA coding for the ROP2 protein of Toxoplasma gondii

Leyva¹,

Hérión²,

Saavedra³

2001

Parasitology Research

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“…Moreover, titers of IgG1 or IgG2a isotypes were heterogeneous, with some animals producing detectable levels of antigen-specific antibodies, whereas the majority appeared not to respond; this is a phenomenon already reported for other DNA vaccines (16,53). Surprisingly, B. ovis infection was unable to boost this response.…”

Section: Discussionmentioning

confidence: 56%

A DNA Vaccine Coding for theBrucellaOuter Membrane Protein 31 Confers Protection againstB. melitensisandB. ovisInfection by Eliciting a Specific Cytotoxic Response

et al. 2005

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“…Several previously described DNA vaccines (21,26) induced antibody titers lower than that induced by the pcDNA-BLS employed in this work. This difference should be attributed to the polymeric structure of BLS.…”

Section: Resultsmentioning

confidence: 64%

“…Plasmid DNA vaccination can protect against many viral and protozoal diseases in animal models (23,26,32). The effect against bacterial infections is less well documented.…”

mentioning

confidence: 99%

A DNA Vaccine Encoding Lumazine Synthase fromBrucella abortusInduces Protective Immunity in BALB/c Mice

Velikovsky¹,

Cassataro²,

Giambartolomei³

et al. 2002

Infect Immun

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This study was conducted to evaluate the immunogenicity of the Brucella abortus lumazine synthase (BLS) gene cloned into the pcDNA3 plasmid, which is driven by the cytomegalovirus promoter. Injection of plasmid DNA carrying the BLS gene (pcDNA-BLS) into BALB/c mice elicited both humoral and cellular immune responses. Antibodies to the encoded BLS included immunoglobulin G1 (IgG1) IgG2a, IgG2b, IgG3, and IgM isotypes. Animals injected with pcDNA-BLS exhibited a dominance of IgG2a over IgG1. In addition, spleen cells from vaccinated animals produced interleukin-2 and gamma interferon but not IL-10 or IL-4 after in vitro stimulation with recombinant BLS (rBLS), suggesting the induction of a Th1 response. Protection was evaluated by comparing the levels of infection in the spleens of vaccinated mice challenged with B. abortus 544. Immunization with pcDNA-BLS-reduced the bacterial burden relative to those in the control groups. Mice immunized with rBLS produced a significant humoral response but did not show a specific cellular response or any protection from challenge. Altogether, these data suggest that pcDNA-BLS is a good immunogen for the production of humoral and cell-mediated responses in mice and is a candidate for use in future studies of vaccination against brucellosis.Brucella abortus is a gram-negative, facultative, intracellular bacterium that infects both cattle and humans, causing abortion and infertility in the former and undulant fever, endocarditis, arthritis, and osteomyelitis in the latter (35).In cattle, variable protective efficacy against brucellosis is obtained by vaccination with live attenuated B. abortus S19 (smooth) or strain RB51 (rough). Although the mechanisms of protection that are induced by attenuated strains are unknown, it is generally accepted that immunity to Brucella is due to antibody-and cell-mediated mechanisms (2,5,18). Th1 immune responses, characterized by production of gamma interferon (IFN-␥), are associated with protective immunity to Brucella. Zhan and colleagues (36, 37) have found that infection of mice with live B. abortus S19 induced a high percentage of IFN-␥-producing Th1 cells, while injection of Brucella protein extracts induced interleukin-4 (IL-4) producing Th2 cells. However, attenuated vaccines are far from being ideal, as they can cause disease in humans and abortion when administered to pregnant cattle. Moreover, because B. abortus S19 induces antibodies to smooth lipopolysaccharide (LPS), it is difficult to differentiate vaccinated animals from naturally infected animals (3, 25). Therefore, the development of better vaccines is necessary for disease control.Immunization with plasmid DNA, consisting of a bacterial plasmid that includes a viral promoter and the gene of interest, represents a promising method in vaccine research. Plasmid DNA vaccination can protect against many viral and protozoal diseases in animal models (23,26,32). The effect against bacterial infections is less well documented. For tuberculosis, independent studies with mice have demonst...

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Comparison of nucleic acid and protein immunization for induction of antibodies specific for HIV-1 gp120

Cited by 23 publications

References 31 publications

Genetic immunization with plasmid DNA coding for the ROP2 protein of Toxoplasma gondii

Genetic immunization with plasmid DNA coding for the ROP2 protein of Toxoplasma gondii

A DNA Vaccine Coding for theBrucellaOuter Membrane Protein 31 Confers Protection againstB. melitensisandB. ovisInfection by Eliciting a Specific Cytotoxic Response

A DNA Vaccine Encoding Lumazine Synthase fromBrucella abortusInduces Protective Immunity in BALB/c Mice

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