Comparison of nonstructural protein-1 antigen detection by rapid and enzyme-linked immunosorbent assay test and its correlation with polymerase chain reaction for early diagnosis of dengue

Gaikwad, Seema; Sawant, Sandhya; Shastri, Jayanthi

doi:10.4103/0974-2727.208265

Cited by 16 publications

(26 citation statements)

References 11 publications

(16 reference statements)

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“…The dengue proportion of 11.1% detected in our study is underestimated because patients with dengue-positive RDTs were excluded from the study. The known sensitivity (6.4–81.5%) of the RDTs could lead to the misdiagnosis of dengue infection cases [35,36]. A clinical analysis focusing on 28 patients with dengue monoinfection showed almost nonspecific manifestations, which were difficult to distinguish from other causes, even in two patients with petechiae (subcutaneous haemorrhages).…”

Section: Discussionmentioning

confidence: 99%

Prospective case-control analysis of the aetiologies of acute undifferentiated fever in Vietnam

Le-Viet

Chung

et al. 2019

Emerging Microbes & Infections

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Acute undifferentiated fever (AUF) is frequently observed in tropical settings, but diagnosing the cause of AUF is often a challenge for local physicians and the physicians treating returning travellers. We conducted a case-control study in central Vietnam in 2016. A total of 378 febrile adult patients (AUFs) with a fever for ≤21 days, no evidence of localized infection and negative screening tests for dengue and malaria, and 384 afebrile adult patients (Controls) were prospectively enrolled. Whole blood, plasma, eschar swab, throat swab and urine specimens were collected and analysed. Quantitative PCR and RT-PCR were used to test for 55 bacteria, viruses and their subtypes. Serological tests were also used to test for rickettsial agents. The most common aetiology was influenza virus (20.9% in AUFs vs. 0% in Controls), followed by rickettsial agents (mainly Orientia tsutsugamushi and Rickettsia typhi ) (10.8% vs. 0.3%), dengue virus (7.7% vs. 0.5%), Leptospira (4.8% vs. 0.8%), adenovirus (4.8% vs. 1.0%), and enterovirus (2.1% vs. 0%) ( p < .05). The real proportion of dengue in AUF cases was underestimated because patients with dengue-positive rapid diagnosis tests were excluded from the study. The emerging agent Rickettsia felis , which had not been previously observed in Vietnam, was detected in this study. In total, 216 patients (57.1%) were given causative diagnoses, comprising 143 (66.2%) monoinfections and 73 (33.8%) coinfections. The infections caused by these agents should be considered in clinical practice and further studies. Additionally, agents susceptible to doxycycline were detected in 15.6% of AUFs; thus, this drug should be included in the panel used to treat AUF patients.

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Section: Discussionmentioning

confidence: 99%

Prospective case-control analysis of the aetiologies of acute undifferentiated fever in Vietnam

Le-Viet

Chung

et al. 2019

Emerging Microbes & Infections

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“…Similarly, the rapid NS1 antigen test allows early DENV detection at points of care. However, the sensitivity of the rapid NS1 antigen detection test was relatively low on days 1 and 2 and after day 5 postsymptomatic onset compared to that seen with the RT-PCR methods (25)(26)(27). In addition, rapid NS1 antigen detection had lower sensitivity in secondary infections, probably due to the interference presented by anti-NS1 antibodies in the patient (49,50); the rapid NS1 antigen detection methods also appeared to have lower sensitivity for certain serotypes, e.g., DENV-2 and -4 (28,29).…”

Section: Discussionmentioning

confidence: 90%

“…For these regions, highly sensitive and specific detection tools that are rapid and affordable for the detection of DENV RNA are needed in primary care facilities. A variety of isothermal amplification methods, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification, have also been developed for these purposes (1,14,25,31,32,40,42,47,52,53). However, since LAMP methodology relies on the use of generic fluorescent dyes or turbidity, its limitations included the propensity to result in false-positive reactions and variations in visual interpretations of LAMP signals among different observers (54).…”

Section: Discussionmentioning

confidence: 99%

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Validation of the Pockit Dengue Virus Reagent Set for Rapid Detection of Dengue Virus in Human Serum on a Field-Deployable PCR System

et al. 2018

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24Dengue virus (DENV) infection, a mosquito-borne disease, is a major public 25 health problem in tropical countries. Point-of-care DENV detection with good 26 sensitivity and specificity enables timely early diagnosis of DENV infection, 27 facilitating effective disease management and control particularly at regions of low 28 resource. POCKIT TM Dengue Virus Reagent Set (GeneReach Biotech), a reverse 29 transcription-insulated isothermal PCR (RT-iiPCR), is available to detect all four 30 serotypes of DENV on the field-deployable POCKIT TM system, which is ready for 31 on-site applications. In this study, analytical and clinical performances of the assay 32 those of the reference qRT-PCR, the index PCR assay on the field-deployable system 44 can serve as a highly sensitive and specific on-site tool for DENV detection. 45 on May 13, 2018 by guest

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“…However, they are reported as less sensitive than ELISA and may cross react with other flaviviruses [11][12][13]. Moreover, their sensitivities were relatively low on days 1 and 2 and after day 5 postsymptomatic onset in human, compared to that seen with the RT-PCR methods [40].…”

Section: Discussionmentioning

confidence: 98%

A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos

Tsai¹,

Liu²,

Lin³

et al. 2019

PLoS ONE

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Background The insulated isothermal PCR (iiPCR) technology enables consistent PCR amplification and detection in a simple heating device. A pan-dengue virus (DENV) RT-iiPCR, targeting the 5’ untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate devices for nucleic acid extraction and PCR amplification/detection) to offer performance comparable to a laboratory real-time PCR. Working on the same technologies, a compact automated sample-in-answer-out system (POCKIT Central Nucleic Acid Analyser) has been available commercially for iiPCR, minimizing human error risks and allowing easy molecular bio-detection near points of need. Here, we evaluated the analytical and clinical performance of the pan-DENV RT-iiPCR on the fully automated system by comparison to those on the semi-automated system. Methodology/Principal findings Testing sera containing serial diluted DENV-1, -2, -3, or -4 cell culture stock, the pan-DENV RT-iiPCR system had similar 100% detection endpoints on the two systems; i.e. at 1, 10, 1 and 10 PFU/ml, respectively, on the fully automated system, and at 10, 1, 10 and 10 PFU/ml, respectively, on the semi-automated system. Furthermore, both fully automated and semi-automated PCR system can detect all four DENV serotypes in mosquitos. Clinical performance of the reagent on the two systems was evaluated by testing 60 human serum samples. Both systems detected the same 40 samples (ten DENV-1, -2, -3, and -4 positive each) and did not detect the other 20; 100% agreement (κ = 1) was found between the two systems. Conclusions/Significance With performance comparable to a previously validated system, the fully-automated PCR system allows applications of the pan-DENV reagent as a useful tool near points of need to facilitate easy, fast and effective detection of dengue virus and help mitigate versatile public health challenges in the control and management of dengue disease.

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Comparison of nonstructural protein-1 antigen detection by rapid and enzyme-linked immunosorbent assay test and its correlation with polymerase chain reaction for early diagnosis of dengue

Cited by 16 publications

References 11 publications

Prospective case-control analysis of the aetiologies of acute undifferentiated fever in Vietnam

Prospective case-control analysis of the aetiologies of acute undifferentiated fever in Vietnam

Validation of the Pockit Dengue Virus Reagent Set for Rapid Detection of Dengue Virus in Human Serum on a Field-Deployable PCR System

A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos

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