Comparison of nitrogen‐mustard‐sensitive and ‐resistant yoshida sarcomas

The Absorption, Distribution and Excretion of 3H-Chlorambucil in Rats Bearing the Yoshida Ascites Sarcoma

“…whether under these conditions it retains an active mustard group). The fact that peak cellular binding of tritium in sensitive cells occurred only 12 hours after treatment, which is much later than with Melphalan (Ball et al, 1966), may also serve to emphasize individual differences between alkylating agents and could be related to the much delayed effects that chlorambucil, in contrast to Melphalan, has on the growth rates of the chlorambucil-sensitive Yoshida ascites cells (Harrap and Hill, 1969): in drug-sensitive tumour-bearing animals, no effect on the growth is observed until 24 hours after treatment.…”

The selectivity of action of alkylating agents and drug resistance. I. Biochemical changes occurring in sensitive and resistant strains of the Yoshida ascites sarcoma following chemotherapy.

“…Thiol-containing compounds have been used to mitigate the toxic effects of alkylating agents (Therkelsen, 1958(Therkelsen, , 1961Connors et al, 1965). Some authors have demonstrated an increase in the non-protein sulphydryl content of nitrogen mustard-resistant Yoshida sarcomata (Hirono, 1961;Hirono et al, 1962;Ball et al, 1966). Furthermore, the sensitivity of a tumour to chemotherapy with alkylating agents may be associated with the ratio protein bound: protein free sulphydryl groups, since in a range of transplantable tumours of varying sensitivity to alkylating agents, the more resistant tumours possessed a higher ratio of protein-free to protein-bound thiol (Calcutt and Connors, 1963).…”

Combined effect of buthionine sulfoximine and cyclophosphamide upon murine tumours and bone marrow

“…In fact, the relationship between the sensitivity of tumour cells to nitrogen mustard and their sulfhydryl contents has been demonstrated by several investigators (Hirono, 1961;Calcutt & Connors, 1963;Ball et al, 1966;Goldberg, 1969;Morita, 1973;Begleiter et al, 1983). Additionally, the increased sensitivity of cells to melphalan following either the incubation of cells in cysteine-deficient medium or exposure to a non-protein sulfhydryl (NPSH) depleting agent, such as diethyl maleate (DEM) or buthionie sulfoximine (BSO), has been reported by several groups (Suzukake et al, 1982;Taylor et al, 1982;Green et al, 1984).…”