Comparison of neural stem/progenitor cells from adult Gecko japonicus and mouse spinal cords

Fang, Shu; Xu, Man; Teng, Long; Lv, Yan; Yang, Jian; Mao, Zuming; Wang, Yin; He, Wei; Wu, Ronghua; Liu, Mei

doi:10.1016/j.yexcr.2019.111812

Cited by 1 publication

(1 citation statement)

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“…In the present study, we obtained 882,273 CCSs, identified 746,317 FLNC, and then yielded 212,964 corrected isoforms with an average read length of 4,153 bp. Compared with short-read sequencing (e.g., Illumina sequencing), the mean length of SMRT-sequenced transcripts was greater than 3 kb, which far exceeded the value reported in previous studies analyzing Heloderma horridum horridum [32], Gekko japonicas [33], Palaemon serratus [34], and Henosepilachna vigintioctopunctata [35]. Furthermore, 117,888 high-quality unique full-length transcripts were generated based on the high competence of PacBio SMRT sequencing, and 111,372 transcripts were successfully annotated with 116,913 ORFs.…”

Section: Plos Onementioning

confidence: 56%

SMRT sequencing of the full-length transcriptome of Gekko gecko

et al. 2022

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Tokay Gecko (Gekko gecko) is a rare and endangered medicinal animal in China. Its dry body has been used as an anti-asthmatic agent for two thousand years. To date, the genome and transcriptome of this species remain poorly understood. Here, we adopted single molecule real-time (SMRT) sequencing to obtain full-length transcriptome data and characterized the transcriptome structure. We identified 882,273 circular consensus (CCS) reads, including 746,317 full-length nonchimeric (FLNC) reads. The transcript cluster analysis revealed 212,964 consensus sequences, including 203,994 high-quality isoforms. In total, 111,372 of 117,888 transcripts were successfully annotated against eight databases (Nr, eggNOG, Swiss-Prot, GO, COG, KOG, Pfam and KEGG). Furthermore, 23,877 alternative splicing events, 169,128 simple sequence repeats (SSRs), 10,437 lncRNAs and 7,932 transcription factors were predicted across all transcripts. To our knowledge, this report is the first to document the G. gecko transcriptome using SMRT sequencing. The full-length transcript data might accelerate transcriptome research and lay the foundation for further research on G. gecko.

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