“…According to their results, sensitivity and specificity of 18S rRNA based primers were 91.9% and 100%, respectively and the sensitivity and specificity of dhfr-ts based primers were 51.4% and 100%, respectively (24). Regarding research on nested PCR, there are several studies reporting that genus-specific rPLU1, rPLU5 based primers and species-specific based rFAL1, rFAL2, rVIV1, rVIV2, rOVA1, rOVA2 and rMAL1, rMAL2 primers had high sensitivity and specificity (5,7,15,(23)(24)(25). In addition, there is also a wide range of studies reporting that especially genus-specific rPLU5, rPLU6 based primers and species-specific based rFAL1, rFAL2, rVIV1, rVIV2, rOVA1, rOVA2 and rMAL1, rMAL2 primers had high sensitivity and specificity in the nested PCR (1,6).…”