2017
DOI: 10.11648/j.ejcbs.20170305.11
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Comparison of Nested PCR and Conventional Analysis of <i>Plasmodium</i> Parasites in Kano, Nigeria

Abstract: Plasmodium identification represents the crucial factor in malaria diagnosis and treatment across developing countries. Conventional microscopy and the use of rapid diagnostic kits have been extensively applied towards human malaria diagnosis. Recombinant DNA techniques have been applied towards malaria diagnosis as well as in the species specific identification using Plasmodium 18s-rRNA gene. This study was undertaken amongst patients attending the Murtala Mohammed Specialist Hospital, Kano. Blood samples wer… Show more

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(2 citation statements)
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“…A PCR study in Plasmodium species conducted in the US showed real-time PCR was a reliable confirmatory tool for the diagnosis of malaria to resolve conflicting doubts between light microscopy and RDT [ 34 ]. A research work in Nigeria found the sensitivity and specificity of PCR to be 100%, whereas 58%–60% of the cases were falsely diagnosed by microscopy and RDT [ 37 ]. False diagnosis by light microscopy happens due to several factors based on experience as well as improper reagent quality [ 9 , 38 ].…”
Section: Discussionmentioning
confidence: 99%
“…A PCR study in Plasmodium species conducted in the US showed real-time PCR was a reliable confirmatory tool for the diagnosis of malaria to resolve conflicting doubts between light microscopy and RDT [ 34 ]. A research work in Nigeria found the sensitivity and specificity of PCR to be 100%, whereas 58%–60% of the cases were falsely diagnosed by microscopy and RDT [ 37 ]. False diagnosis by light microscopy happens due to several factors based on experience as well as improper reagent quality [ 9 , 38 ].…”
Section: Discussionmentioning
confidence: 99%
“…According to their results, sensitivity and specificity of 18S rRNA based primers were 91.9% and 100%, respectively and the sensitivity and specificity of dhfr-ts based primers were 51.4% and 100%, respectively (24). Regarding research on nested PCR, there are several studies reporting that genus-specific rPLU1, rPLU5 based primers and species-specific based rFAL1, rFAL2, rVIV1, rVIV2, rOVA1, rOVA2 and rMAL1, rMAL2 primers had high sensitivity and specificity (5,7,15,(23)(24)(25). In addition, there is also a wide range of studies reporting that especially genus-specific rPLU5, rPLU6 based primers and species-specific based rFAL1, rFAL2, rVIV1, rVIV2, rOVA1, rOVA2 and rMAL1, rMAL2 primers had high sensitivity and specificity in the nested PCR (1,6).…”
Section: Discussionmentioning
confidence: 99%