Comparison of neonatal reference intervals for 23 biochemical analytes in the cord blood-A single center study in South Korea

Abstract

Choi SJ, Lee S, Lee B, Jang JY, Cho J, Uh Y. Comparison of neonatal reference intervals for 23 biochemical analytes in the cord blood-A single center study in South Korea. Turk J Pediatr 2019; 61: 337-344. Reference intervals for laboratory tests are important in the diagnosis and treatment of disease. However, due to difficulty in recruiting sufficient numbers of reference subjects, studies regarding reference intervals for biochemical analytes in neonates is lacking. The aim of this study was to compare and validate the reference intervals for 23 biochemical analytes in the cord blood of neonates. From August to December 2017, 79 consecutive neonates born at the Wonju Severance Christian Hospital (Wonju, Korea) with C-reactive protein concentration less than 0.05 mg/dL were included in this study. All of 23 biochemical analytes were measured by the cobas 8000 c702 (Roche Diagnostics, Switzerland). Mean ± 2 standard deviations (SD) were calculated if the values were normally distributed, and median and the range of 2.5-97.5 percentile were described when the values were not normally distributed. We compared the neonatal reference intervals for 23 biochemical analytes. Alkaline phosphatase and gamma glutamyl transferase showed significant differences according to sex, and direct bilirubin revealed significant differences depending on the delivery mode. These compared reference intervals of 23 biochemical analytes will be useful for clinical decision-making in the management of neonates.

Reference Intervals for 26 Common Biochemical Analytes in Term Neonates in Jilin Province, China

“…This helps avoid exaggerated SDR for the actual narrow range of RIs or masked differences caused by the interaction between the factors. [2,21] In effect, no sex difference for all analytes was detected , which was consistent with data in different regions [8,17,22].…”

“…These results showed that some differences in LDH were quite marked among the RIs listed for similar age group. ULs of Ca, Mg, Fe, TCHO and HDL-C are signi cantly lower than those from the experiment based on the cord blood in Korean [17]. The RIs of most analytes are similar to those from the study in Lanzhou, China [18], however the RIs of liver enzymes except CHE showed great differences compared with those from Zhengzhou, China [19].…”

Reference intervals for 26 common biochemical analytes in term neonates in Jilin Province, China

“…Therefore, this study statistically depended upon SDRdm, SDR-sex and SDR-age to analyze differences interaction between the factors [2,28]. In effect, no sex difference for all analytes was detected, which was consistent with data in different regions [5,11,13]. However, there were some considerable differences in postnatal age.…”

“…These results showed that some differences in LDH were quite marked among the RIs listed for similar age groups. ULs of Ca, Mg, Fe, TCHO and HDL-C are significantly lower than those from the experiment based on the cord blood in Korean [13]. The RIs of most analytes are similar to those from the study in Lanzhou, China [15], however the RIs of liver enzymes except CHE showed great differences compared with those from Zhengzhou, China [26].…”

“…The Ministry of Health P. R. China has published several industry standards which provide RIs for common clinical biochemistry and immunology tests in adults, while data about newborns are not available. A few recent studies have established neonatal RIs for biochemistry analytes based on the local population, preterm and low-birth-weight infants included [11,[13][14][15]. However, most clinical laboratories in China adopt RIs from textbooks, reagent inserts, or adult reference standards.…”