Comparison of myotoxic effects of levobupivacaine, bupivacaine and ropivacaine: apoptotic activity and acute effect on pro-inflammatory cytokines

Gergin, Özlem Öz; Bayram, Adnan; Gergin, I S; Aksu, Recep; Yay, Arzu; Balcıoğlu, Esra; Polat, Sait; Çoşkun, Gülfidan; Sarıca, Zeynep Soyer; Yildiz, Karamehmet

doi:10.1080/10520295.2018.1548711

Cited by 19 publications

(16 citation statements)

References 32 publications

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“…Bupivacaine is an amide, which is a long-acting local anesthetic commonly administered in various procedures, such as labor analgesia and total knee arthroplasty. Bupivacaine has the advantage of rapid onset of action and is relatively safe at the recommended dosage [5]. However, overdose or accidental intravasal application of bupivacaine can result in significant adverse effects.…”

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confidence: 99%

Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function

et al. 2020

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Background: Electroacupuncture is well known for its advantageous neuroanalgesic and therapeutic effects on myocardial ischemia-reperfusion injury. The purpose of the present research was to verify whether electroacupuncture can alleviate bupivacaine-induced myocardial injury. Methods: Specific pathogen-free Wistar rats were used to establish the bupivacaine-induced myocardial injury model. Western blot, PCR, transmission electron microscope and enzyme-linked immunosorbent (ELISA) methods were used to evaluate bupivacaine-induced structure injury and dysfunction of the mitochondria as well as the alleviating effects of lipid emulsion, acupoint injection, and electroacupuncture pre-treatment of the oxidase stress response. Results: Bupivacaine caused structural damage, degradation, and swelling of mitochondria. Furthermore, it reduced adenosine triphosphate (ATP) synthesis and impaired energy metabolism in the mitochondria. Structural and functional impairment of the mitochondria was alleviated via lipid emulsion injection, acupoint injection, and electroacupuncture pre-treatment. Electroacupuncture pre-treatment of PC6 yielded a greater alleviating effect than others approaches. Following electroacupuncture pre-treatment of PC6 point, the number of mitochondria increased; apoptosis was reduced, enzymatic activity of cytochrome C oxidase (COX) and superoxide dismutase and expression of uncoupling protein 2, voltage-dependent anion channel 1, and Bcl 2 were upregulated and SLC25A6, MDA levels were downregulated. Additionally, our findings indicated that electroacupuncture pre-treatment of PC6 point exerted an effect on the mitochondria via the mitochondrial-transcription-factor-A/nuclear-respiratory-factor-1/proliferatoractivated-receptor-gamma-coactivator-1 pathway. Conclusion: The present study revealed that electroacupuncture pre-treatment of PC6 could effectively alleviate bupivacaine-induced myocardial mitochondrial damage, thereby providing a theoretical basis for clinical studies and applications of this treatment method.

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confidence: 99%

Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function

et al. 2020

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“…Bupivacaine is an amide, which is a long-acting local anaesthetic commonly administered in various procedures, such as labour analgesia and total knee arthroplasty. It has the advantage of rapid onset of action [4]. However, it also results in significant adverse effects.…”

Section: Introductionmentioning

confidence: 99%

“…However, it also results in significant adverse effects. Reportedly, bupivacaine induce muscle toxicity in skeletal muscles [4]. Bupivacaine-induced cardiotoxicity may result in cardiac arrhythmia, poor myocardial contractility and cardiac arrest due to circulatory collapse [5,6].…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture preconditioning alleviated myocardial injury via regulating mitochondrial function

Wang

Liang

et al. 2020

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BackgroundIn clinical research, integrated Chinese and Western medicine has been increasing accepted due to the advantages. Clinical studies related to bupivacaine-induced myocardial injury are considered indispensable. Therefore, the purpose of this study is to verify whether acupuncture preconditioning could alleviate side effect of bupivacaine-induced. MethodsSimulating bupivacaine-induced myocardial injury in clinical surgery, western blot, PCR, transmission electron microscope, Elisa methods were used to evaluate the bupivacaine-induced structure injury and dysfunction of mitochondria, and alleviating effect of lipid emulsion, acupoint injection, and electroacupuncture pre-treatment oxidase stress response. ResultsBupivacaine caused structural damage, degradation, and swelling of mitochondria. Furthermore, it reduced adenosine triphosphate (ATP) synthesis and impaired energy metabolism in mitochondria. Structural and functional impairment of mitochondria were alleviated via lipid emulsion injection, acupoint injection, and electroacupuncture pre-treatment. Electroacupuncture pre-treatment yielded a greater alleviating effect than the other two approaches. Following electroacupuncture pre-treatment, the number of mitochondria increased, apoptosis was reduced, enzymatic activity of cytochrome C oxidase and superoxide dismutases, expression of uncoupling protein 2, Voltage-dependent anion channel 1, and Bcl 2 were upregulated and SLC25A6, MDA level was downregulated. Additionally, our findings indicated that electroacupuncture pre-treatment exerted an effect on mitochondria via the mitochondrial transcription factor A / nuclear respiratory factor 1 / proliferator-activated receptor-gamma coactivator -1 pathway. ConclusionOur study revealed that electroacupuncture pre-treatment could effectively alleviate bupivacaine-induced myocardial mitochondrial damage, thereby providing a theoretical basis for clinical studies and application of this treatment method.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Bupivacaine reportedly induces muscle toxicity in skeletal muscles [5]. Bupivacaine-induced cardiotoxicity may result in cardiac arrhythmia, poor myocardial contractility and cardiac arrest due to circulatory collapse [7,8].…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function

Wang

Liang

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Electroacupuncture is well known for its advantageous neuroanalgesic and therapeutic effects on myocardial ischemia-reperfusion injury. The purpose of the present research was to verify whether electroacupuncture can alleviate bupivacaine-induced myocardial injury. Methods Specific-pathogen-free Wistar rats were used to establish the bupivacaine-induced myocardial injury model. Western blot, PCR, transmission electron microscope and enzyme-linked immunosorbent (ELISA) methods were used to evaluate bupivacaine-induced structure injury and dysfunction of the mitochondria as well as the alleviating effects of lipid emulsion, acupoint injection, and electroacupuncture pre-treatment of the oxidase stress response. Results Bupivacaine caused structural damage, degradation, and swelling of mitochondria. Furthermore, it reduced adenosine triphosphate (ATP) synthesis and impaired energy metabolism in the mitochondria. Structural and functional impairment of the mitochondria was alleviated via lipid emulsion injection, acupoint injection, and electroacupuncture pre-treatment. Electroacupuncture pre-treatment of PC6 yielded a greater alleviating effect than others approaches. Following electroacupuncture pre-treatment of PC6 point, the number of mitochondria increased; apoptosis was reduced, enzymatic activity of cytochrome C oxidase (COX) and superoxide dismutase and expression of uncoupling protein 2, voltage-dependent anion channel 1, and Bcl 2 were upregulated and SLC25A6, MDA levels were downregulated. Additionally, our findings indicated that electroacupuncture pre-treatment of PC6 point exerted an effect on the mitochondria via the mitochondrial-transcription-factor-A / nuclear-respiratory-factor-1 / proliferator-activated-receptor-gamma-coactivator -1 pathway. Conclusion The present study revealed that electroacupuncture pre-treatment of PC6 could effectively alleviate bupivacaine-induced myocardial mitochondrial damage, thereby providing a theoretical basis for clinical studies and applications of this treatment method.

show abstract

Comparison of myotoxic effects of levobupivacaine, bupivacaine and ropivacaine: apoptotic activity and acute effect on pro-inflammatory cytokines

Cited by 19 publications

References 32 publications

Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function

Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function

Electroacupuncture preconditioning alleviated myocardial injury via regulating mitochondrial function

Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function

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