Comparison of Myeloperoxidase Activity in Leukocytes from Normal Subjects and Patients with Chronic Granulomatous Disease

Pegram, P. Samuel; DeChatelet, Lawrence R.; McCall, Charles E.

doi:10.1093/infdis/138.5.699

Cited by 3 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…17 In the phagocytes of patients with CGD, the production of ROS is disturbed due to a defect in NADPH oxidase, 18 whereas MPO is active in azurophilic granules. 11 This was demonstrated again for the CGD granulocytes and monocytes used in this study.…”

Section: Discussionsupporting

confidence: 71%

See 1 more Smart Citation

Reconstitution of bactericidal activity in chronic granulomatous disease cells by glucose-oxidase–containing liposomes

Gerber

Bruchelt

Falk

et al. 2001

Blood

View full text Add to dashboard Cite

Chronic granulomatous disease (CGD) is an inherited primary immunodeficiency characterized by phagocytes devoid of a functioning nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. The failure of CGD phagocytes to produce reactive oxygen species (ROS) results in a marked increase in the susceptibility of affected patients to life-threatening bacterial and fungal infections. This study investigated whether loading of CGD phagocytes with glucose oxidase (GO)-containing liposomes (GOLs) could restore cellular production of bactericidal ROS (eg, H 2 O 2 and HOCl) in vitro. Results indicate that GO encapsulated in liposomes enabled NADPH oxidase-deficient phagocytes to use H 2 O 2 for the production of highly bactericidal HOCl. The intracellular colocalization of bacteria and liposomes (or liposome-derived ferritin) was demonstrated by confocal laser microscopy and electron microscopy. After uptake of GOLs (approximately 0.2 U/mL at 1 mM total lipid concentration, size approximately 180 nm), CGD granulocytes produced HOCl levels comparable to those of normal phagocytes. Remarkably, after treatment with GOLs, CGD phagocytes killed Staphylococcus aureus as efficiently as normal granulocytes. Moreover, treated cells retained sufficient motility toward chemotactic stimuli as measured by chemotaxis assay. Side effects were evaluated by measuring the H 2 O 2 concentrations and the production of methemoglobin in whole blood. These studies revealed that H 2 O 2 produced by GOLs was degraded immediately by the antioxidative capacity of whole blood. Elevated methemoglobin levels were observed only after application of extremely high amounts of GOLs (2 U/mL). In summary, the application of negatively charged GOLs might provide a novel effective approach in the treatment of patients with CGD at high risk for lifethreatening infections. IntroductionChronic granulomatous disease (CGD) is a primary immunodeficiency with an incidence of 1:1 000 000 to 1:250 000. 1 It is an inherited disorder (X chromosomal or autosomal recessive) of the phagocyte system. 2 Phagocytes (granulocytes and monocytes) of patients with CGD lack a functional nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and are not able to produce reactive oxygen species (ROS), necessary for microbial killing. 3 The disease is therefore dominated by high susceptibility to a variety of bacteria and fungi, especially those with catalase activity. 4 Therapy and prevention of infections include the life-long administration of antibiotics and antimycotics, and in severe cases, the transfusion of allogeneic granulocytes to provide the patient with functioning phagocytes. 5 In recent years the idea of encapsulating drugs such as antibiotics or cytostatics into liposomal carriers has become more feasible. 6 Phagocytes tend to take up conventional liposomes very easily. 7 This uptake is often a disadvantage in the use of liposomes for many medical applications and clinical trials because of rapid elimination of the encapsulated drug. We developed glucose oxid...

show abstract

Section: Discussionsupporting

confidence: 71%

“…In the case of CGD, therapeutic application of GOLs for the restoration of ROS production could be useful because phagocytes of patients with CGD have a normal myeloperoxidase (MPO) activity. 11 The GO-derived H 2 O 2 can be converted within CGD phagocytes into the strong oxidant HOCl during the MPO reaction:…”

Section: Introductionmentioning

confidence: 99%

Reconstitution of bactericidal activity in chronic granulomatous disease cells by glucose-oxidase–containing liposomes

Gerber

Bruchelt

Falk

et al. 2001

Blood

View full text Add to dashboard Cite

show abstract

“…Killing ability of leukocytes chronic granulomatous disease ( from a patient with CGD dem( paired and delayed killing of n A hydrogen peroxide-generatin the CGD larvicidal assay to ei cyte enzymes such as peroxida in CGD leukocytes (26), might hydrogen peroxide in larval kil in this assay by hydrogen peroxic glucose oxidase with glucose of 0.12 mU/test or greater (27) ter preincubation with 6 h incubation at 4°C, circumferential fluores-37°C, all fluorescence ubated in normal serum at any time.…”

Section: Resultsmentioning

confidence: 99%