Comparison of multiple assays for detecting human antibodies directed against surface antigens on normal and malignant human tissue culture cells

Rosenberg, Steven A.; Schwarz, Susan; Anding, Hedi; Hyatt, Cornelia; Williams, Gary M.

doi:10.1016/0022-1759(77)90105-3

Cited by 12 publications

(1 citation statement)

References 11 publications

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“…There exist numerous methods of potential value for detecting antibodies to tumor antigens in xenogeneic antisera prepared against cellular antigens (Ting and Herberman, 1976;Herberman, 1977). The array of technologies employed previously includes cytotoxicity (Schultz et al, 1975;Fritz e et al, 1976, Rosenberg et al, 1977, complement fixation (Lewis et al, 1969;Wood and Barth, 1974;Sabin and Tarro, 1973;Hollinshead et al, 1976;Rogers et al, 1977), isotopic antiglobulin binding assays (Harder and MacKhann, 1968;Sparks et al, 1969), immunofluorescence (Henle and Henle, 1966;Moller, 1961;McCoy et al, 1974) and antibody-dependent cellular cytoxicity (Perlman et al, 1972;Jolley et al, 1976). The discovery by Kronvall et al, (1970) that Staphylococcus aureus protein A (SPA can bind the Fc portion of several mammalian species of IgG provided the basis for development of new immunoassays to detect IgG antibodies and cellular antigens.…”

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confidence: 99%

Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma

Veltri

McKolanis

Rockoff

et al. 1980

Intl Journal of Cancer

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Xenoantisera were prepared in rabbits against intact paraformaldehyde-fixed Lewis lung carcinoma (LLCa) cells and membrane solubilized proteins extracted with Triton X-100 and 3M KCI. The antisera were analyzed for antibodies to tumor-associated membrane antigens (TAMA) by means of a solid-phase radioimmunoassay (RIA). THe immunoassay employed 125-labelled Staphylococcus aureus protein A (SPA) to detect specific antibody binding to glutaraldehyde-fixed Lewis lung tumor target cells. The protein-A antibody-binding (PAAB) method is rapid, sensitive and reproducible. The PAAB revealed a possible LLCa-TAMA as evidenced by the reactivity of xenoantisera raised to LL paraformaldehyde tumor cell vaccine (TCV) and LL tumor Triton X-100 (TTri) and 3M KCI (TKCI) extracts of membrane preparations. The reactivity of our antisera followed linear dose-response kinetics but the degree of reactivity decreased from anti-TCV to anti-TTri to anti-TKCI respectively. The LL-TAMA demonstrated specificity since our antisera did not significantly cross-react with normal C56BL/6 cellular antigens or other known murine tumor-associated surface antigens of virally and chemically-induced tumor systems.

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confidence: 99%

Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma

Veltri

McKolanis

Rockoff

et al. 1980

Intl Journal of Cancer

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Demonstration of specific serologic reactivity in human osteosarcoma

Sindelar

1983

Cancer

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Cultured cell lines of osteosarcoma and normal skin fibroblasts were derived from tissues of five patients with localized osteosarcoma. Testing of pretreatment patient sera in an indirect immunofluorescence assay against both autologous normal fibroblasts and autologous osteosarcoma cells showed cytoplasmic staining. Quantitative absorption of patient sera with human fetal cells removed all reactivity against autologous fibroblasts, but fetalabsorbed sera showed cytoplasmic, membrane, and nuclear fluorescence when tested against autologous osteosarcoma. Testing the five patient sera against all five cell lines showed staining of allogeneic fibroblasts and osteosarcoma by nonabsorbed sera. Following fetal absorption, all reactivity against fibroblasts was removed, with the retention of specific staining of all osteosarcoma cell lines by all five patient sera. Additional testing of sera from normal patients, patients with soft tissue sarcomas, and patients with melanoma showed staining of allogeneic cultured fibroblasts and tumor cells derived from a patient with osteosarcoma. All staining against fibroblasts and tumor cells was eliminated by fetal absorption of the allogeneic sera. Results suggested that fetal neoantigens are expressed in cultured normal and malignant cell lines and that such fetal antigens are recognized by natural antibodies. Absorption of antifetal reactivity allowed demonstration of specific serum reactivity against osteosarcoma cells.

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Tumor-Associated Blood Group Antigen Expressions and Immunoglobulins Associated with Tumors

Anderson

Davis

Venegas

1988

The Molecular Immunology of Complex Carbohydrates

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Comparison of multiple assays for detecting human antibodies directed against surface antigens on normal and malignant human tissue culture cells

Cited by 12 publications

References 11 publications

Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma

Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma

Demonstration of specific serologic reactivity in human osteosarcoma

Tumor-Associated Blood Group Antigen Expressions and Immunoglobulins Associated with Tumors

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