Comparison of Multi-Lineage Cells from Human Adipose Tissue and Bone Marrow

Ugarte, Daniel A. De; Morizono, Kouki; Elbarbary, Amir; Alfonso, Zeni; Zuk, Patricia A.; Zhu, Min; Dragoo, Jason L.; Ashjian, Peter; Thomas, Brandon J.; Benhaim, Prosper; Chen, Irvin S. Y.; Fraser, John K.; Hedrick, Marc H.

doi:10.1159/000071150

Cited by 1,062 publications

(719 citation statements)

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“…More than 90% of hASC cells expressed CD13, CD90 (Thy-1) and CD105/SH2 (Endoglin); about 90% were CD44 + , around 80% CD29 + , about 75% CD54 (ICAM-1) + and 30-85% expressed CD49d, whereas there was very low expression of CD14 (around 1-4%) and no expression of CD45, CD71 and CD106 was detected, confirming previous published data (De Ugarte et al, 2003;Lee et al, 2004). After surface marker characterization, hASCs were then differentiated towards the osteogenic lineage by culturing them at a density of 15 000 cells/cm 2 in two different media: OM1 (CTRL medium supplemented with 10 mM β-glycerol phosphate, 100 nM dexamethasone, 50 µM ascorbic acid-2-phosphate); and OM2 (CTRL plus 10 mM β-glycerol phosphate, 10 nM dexamethasone and 150 µM ascorbic acid-2-phosphate).…”

Section: Introductionsupporting

confidence: 90%

“…Human adipose-derived stem cells (hASCs) have been shown to possess multilineage potential to differentiate into bone, cartilage, fat, muscle, endothelial and neural cells (Zuk et al, 2001;De Ugarte et al, 2003;Dudas et al, 2006;Dragoo et al, 2003;Planat-Benard et al, 2004;Choi et al, 2006). We here analyse the differentiation capacity of hASCs cultured in two different osteogenic-inductive media.…”

Section: Introductionmentioning

confidence: 99%

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Osteogenic differentiation of human adipose-derived stem cells: comparison of two different inductive media

Girolamo

Sartori

Albisetti

et al. 2007

J Tissue Eng Regen Med

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Human mesenchymal stem cells (MSCs) have the potential to differentiate into cells of connective tissue lineages, including bone, cartilage, fat, muscle and also neurons. In our study we have examined the phenotypic profile of human adipose tissue-derived stem cells (hASCs) and compared different osteogenic-inductive media to assess hASC differentiation. Cells were enzymatically isolated from adipose tissues derived by liposuction from several adult human donors, purified and then expanded in culture. We obtained an abundant yield of hASCs with a constant proliferative trend, a doubling time of about 68 h and a mild variable clonogenic capacity. At passage 4, hASCs expressed MSC-related cell surface antigens (CD13, CD105, CD54, CD90, CD44), and subsequently hASCs were induced to differentiate into the osteogenic lineage for at least 3 weeks of culture in two distinct media, OM1 and OM2, differing in dexamethasone and ascorbic acid concentrations. Osteogenic differentiation of OM1-and OM2-cultured cells was assessed by evaluating cell morphology, osteopontin expression, alkaline phosphatase activity and calcium deposition. OM2 medium showed a higher osteogenic potential than OM1, as assessed by increased levels of calcium deposition, alkaline phospatase activity and osteopontin expression in comparison with OM1-differentiated cells. We conclude that hASCs efficiently differentiate into osteogenic lineage, particularly when cultured in inductive medium supplemented with 10 nM dexamethasone and 150 µM ascorbic acid.

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Section: Introductionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Osteogenic differentiation of human adipose-derived stem cells: comparison of two different inductive media

Girolamo

Sartori

Albisetti

et al. 2007

J Tissue Eng Regen Med

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“…There are increasing reports that MSCs can be isolated from various adult mesenchymal tissues such as synovium (5), periosteum (6), skeletal muscle (7), and adipose tissue (8) in addition to bone marrow (9). These MSCs have been assumed to be similar irrespective of their original tissue source since they all have self-renewal and multidifferentiation potential with common surface epitopes (10,11). However, the properties of MSCs can be affected by their preparations (12)(13)(14), which have not been properly controlled for in some studies.…”

mentioning

confidence: 99%

Comparison of human stem cells derived from various mesenchymal tissues: Superiority of synovium as a cell source

Sakaguchi¹,

Sekiya²,

Yagishita³

et al. 2005

Arthritis & Rheumatism

1,367

1,171

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Objective. To compare the properties of human mesenchymal stem cells (MSCs) isolated from bone marrow, synovium, periosteum, skeletal muscle, and adipose tissue.Methods. Human mesenchymal tissues were obtained from 8 donors during knee surgery for ligament injury. After collagenase digestion or gradient-density separation, nucleated cells were plated at an appropriate density for expansion at the maximum rate without colony-to-colony contact. Yield, expandability, differentiation potential, and epitope profile were compared among MSCs from the 5 different tissue sources.Results. Colony number per 10 3 nucleated cells was lower, and cell number per colony was higher, in bone marrow than in other mesenchymal tissues. When the cells were replated at low density every 14 days, bone marrow-, synovium-, and periosteum-derived cells retained their proliferation ability even at passage 10. In chondrogenesis studies in which the cells were pelleted and cultured in vitro, pellets from bone marrow-, synovium-, and periosteum-derived cells were shown to be larger and stained more extensively for cartilage matrix. Synovium-derived cells, in particular, had the greatest ability for chondrogenesis. In adipogenesis experiments, the frequency of oil red O-positive colonies was highest in synovium-and adipose tissue-derived cells. In studies of osteogenesis, the rate of alizarin red-positive colonies was highest in bone marrow-, synovium-, and periosteum-derived cells. For epitope profiling, 15 surface antigens were measured. Most appeared to have similar epitope profiles irrespective of cell source.Conclusion. Our findings indicate that there are significant differences in MSC properties according to tissue source, beyond donor and experimental variation. Superiority of synovium as a potential source of MSCs for clinical applications was demonstrated.

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“…Consistently, the siRNA signal (by Quasar570 fluorescence) in the 3D-cultured H9 cells dramatically drops 2 d after particle incubation ( Figure S6, Supporting Information), indicating the dilution or degradation of siRNA in cells. We carried out similar experiments with HeLa cells transduced with GFP (≈24 h doubling time) [37] to explore why gene silencing is lost after 3 d in hESC. However, laser irradiation 3 d post HGN internalization still showed effective gene silencing (Figure 4c and Figure S7 (Supporting Information)), indicating loss of siRNA knockdown is likely due to rapid siRNA degradation in hESCs rather than dilution by cell division.…”

Section: Communicationmentioning

confidence: 99%