Comparison of morbillivirus proteins by limited proteolysis

Abstract

Proteins of a number of measles and SSPE virus strains have been compared by limited proteolysis and they appear to be largely conserved amongst the various strains. Viruses derived from SSPE cannot be distinguished from other measles viruses by this technique. Small differences in the digest patterns of the M proteins have been observed between the Edmonston and other measles virus strains. Furthermore, in some strains where the M proteins migrate slower in SDS-PAGE the limited proteolysis patterns are slightly different from those in other MV and SSPE virus strains. The limited proteolysis pattern of some canine distemper virus (CDV) proteins have been determined and nucleocapsid breakdown products have been identified in infected cells. Comparisons of proteins of four strains of CDV have shown that these, too, are largely conserved, although the digest of proteins of CDV appear to show more pronounced differences than those present in the MV and SSPE virus group. Limited proteolysis can be used readily to distinguish MV from CDV isolates.

Identification of Several Different Lineages of Measles Virus

“…The origin and nucleotide sequence of some of the virus strains analysed here have been reported earlier (See Table 1). The origins of strains EdP9, Hu2, MVO and MVP have been described before (Rima et al, 1983). Sequences of the $33 and $81 strains were obtained from brain autopsy material from two cases of SSPE diagnosed in Northern Ireland.…”

The Proteins of Morbilliviruses

“…These two proteins can also be distinguished by two-dimensional tryptic digest fingerprinting and by limited proteolysis Shapshak et al, 1982;Rima et al, 1983).…”

“…It has also been identified in translation experiments in vitro with poly A-containing RNA from MV-infected cells (Niveleau & Wild, 1979;Rozenblatt eta/., 1979;Sprague et aL, 1979). The P proteins of MV and CDV appear to be similar as judged from their immunological cross-reactivity 0rvell & Norrby, 1980) although the proteins can be distinguished by two-dimensional tryptic digest fingerprinting and by limited proteolysis Rima et al, 1983).…”

“…The M proteins of MV and CDV can be distinguished by their different mobilities in SDSpolyacrylamide gels since the M proteins of all CDV strains so far tested migrate faster than the M proteins of MV and SSPE virus strains (Campbell et al, 1980). The M proteins of these two viruses can also be distinguished by two-dimensional tryptic digest fingerprinting and by limited proteolysis Rima et al, 1983). Furthermore, the M proteins of different CDV strains appear to have similar limited proteolysis patterns (Shapshak et al, 1982).…”

The Antigenic Relationship between Measles, Canine Distemper and Rinderpest Viruses Studied with Monoclonal Antibodies

“…Studies on the antigenic relationship of MV to CDV detected pronounced antigenic differences only between the haemagglutinin (H) protein of measles virus and the equivalent protein in CDV (Stephenson & ter Meulen, 1979;Hall et al, 1980;Orvell & Norrby, 0000-6993 © 1986SGM 1980Wild & Huppert, 1980;Shapshak et al, 1982;Russell, 1983). This is in contrast to peptide mapping techniques which showed marked differences between all of the polypeptides of CDV and MV studied (Hall et al, 1980;Rima et al, 1983). Many of these differences probably represent the non-antigenic framework regions.…”