Comparison of mono- and co-infection by swine influenza A viruses and porcine respiratory coronavirus in porcine precision-cut lung slices

Krimmling, Tanja; Schwegmann-Weßels, Christel

doi:10.1016/j.rvsc.2017.07.016

Cited by 13 publications

(13 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SwIAV and PCV2 as primary infectious agents have been less studied. However, it can be observed that swIAV can interfere with other virus productions (PRRSV and PRCoV) [85,86] whereas PCV2 has some detrimental impact on the clinical outcome of secondary viral infections (PRRSV, swIAV, and PPV) [87][88][89] (see Additional file 1 and Figure 1A). Then, regarding the inflammation induced in coinfection conditions, various outcomes were observed depending which viruses were considered (Figure 2A).…”

Section: The Different Types Of Coinfections 321 Virus-virusmentioning

confidence: 99%

Coinfections and their molecular consequences in the porcine respiratory tract

Saadé

Deblanc

Bougon

et al. 2020

Vet Res

145

166

View full text Add to dashboard Cite

Understudied, coinfections are more frequent in pig farms than single infections. In pigs, the term "Porcine Respiratory Disease Complex" (PRDC) is often used to describe coinfections involving viruses such as swine Influenza A Virus (swIAV), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and Porcine CircoVirus type 2 (PCV2) as well as bacteria like Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae and Bordetella bronchiseptica. The clinical outcome of the various coinfection or superinfection situations is usually assessed in the studies while in most of cases there is no clear elucidation of the fine mechanisms shaping the complex interactions occurring between microorganisms. In this comprehensive review, we aimed at identifying the studies dealing with coinfections or superinfections in the pig respiratory tract and at presenting the interactions between pathogens and, when possible, the mechanisms controlling them. Coinfections and superinfections involving viruses and bacteria were considered while research articles including protozoan and fungi were excluded. We discuss the main limitations complicating the interpretation of coinfection/superinfection studies, and the high potential perspectives in this fascinating research field, which is expecting to gain more and more interest in the next years for the obvious benefit of animal health.

show abstract

Section: The Different Types Of Coinfections 321 Virus-virusmentioning

confidence: 99%

Coinfections and their molecular consequences in the porcine respiratory tract

Saadé

Deblanc

Bougon

et al. 2020

Vet Res

145

166

View full text Add to dashboard Cite

show abstract

“…Focusing on the IFN-ω members and their antiviral and inflammatory regulation, we characterized family-wide porcine innate immune IFNs for their functional spectrum and therapeutic potential (9, 10, 13, 14), which was profiled against two RNA viruses: porcine reproductive and respiratory syndrome virus (PRRSV), and influenza A virus. Both viruses have a high impact on the swine industry and influenza A virus in swine, also threatens public health (13, 16). Here, we show that vertebrate IFN-ω subtype has evolved several novel features, which include: (1) being a signature multi-gene subtype expanding particularly in bats and ungulates (7, 9, 17, 18), (2) emerging isoforms that have much higher antiviral potency than typical IFN-α (14, 18, 19), (3) highly cross-species antiviral (but little anti-proliferative) activity exerted in cells of humans and other mammalian species (20), and (4) other potential novel molecular and functional properties (3, 4, 18, 20).…”

Section: Introductionmentioning

confidence: 99%

Cross-Species Genome-Wide Analysis Reveals Molecular and Functional Diversity of the Unconventional Interferon-ω Subtype

et al. 2019

View full text Add to dashboard Cite

Innate immune interferons (IFNs), particularly type I IFNs, are primary mediators regulating animal antiviral, antitumor, and cell-proliferative activity. These antiviral cytokines have evolved remarkable molecular and functional diversity to confront ever-evolving viral threats and physiological regulation. We have annotated IFN gene families across 110 animal genomes, and showed that IFN genes, after originating in jawed fishes, had several significant evolutionary surges in vertebrate species of amphibians, bats and ungulates, particularly pigs and cattle. For example, pigs have the largest but still expanding type I IFN family consisting of nearly 60 IFN-coding genes that encode seven IFN subtypes including multigene subtypes of IFN-α, -δ, and -ω. Whereas, subtypes such as IFN-α and -β have been widely studied in many species, the unconventional subtypes such as IFN-ω have barely been investigated. We have cross-species defined the IFN evolution, and shown that unconventional IFN subtypes particularly the IFN-ω subtype have evolved several novel features including: (1) being a signature multi-gene subtype expanding primarily in mammals such as bats and ungulates, (2) emerging isoforms that have superior antiviral potency than typical IFN-α, (3) highly cross-species antiviral (but little anti-proliferative) activity exerted in cells of humans and other mammalian species, and (4) demonstrating potential novel molecular and functional properties. This study focused on IFN-ω to investigate the immunogenetic evolution and functional diversity of unconventional IFN subtypes, which may further IFN-based novel antiviral design pertinent to their cross-species high antiviral and novel activities.

show abstract

“…Primary porcine tracheal epithelial cells (PTECs) and primary porcine bronchial epithelial cells (PBECs) were harvested from the 5-month-old pigs’ trachea and bronchial, respectively, as previously described [ 19 , 20 ]. Briefly, PTECs and PBECs firstly maintained in bronchial epithelial cell growth medium (Lonza, Basel, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

“…The cells were incubated in a humidified atmosphere containing 5% CO 2 at 37 °C and passaged every 2 to 3 days. PRCoV Bel85 was obtained from Luis Enjuanes [ 20 ]. The virus stock was propagated on ST cells in EMEM.…”

Section: Methodsmentioning

confidence: 99%

The Cell Tropism of Porcine Respiratory Coronavirus for Airway Epithelial Cells Is Determined by the Expression of Porcine Aminopeptidase N

Peng

Punyadarsaniya

Shin

et al. 2020

Viruses

View full text Add to dashboard Cite

Porcine respiratory coronavirus (PRCoV) infects the epithelial cells in the respiratory tract of pigs, causing a mild respiratory disease. We applied air–liquid interface (ALI) cultures of well-differentiated porcine airway cells to mimic the respiratory tract epithelium in vitro and use it for analyzing the infection by PRCoV. As reported for most coronaviruses, virus entry and virus release occurred mainly via the apical membrane domain. A novel finding was that PRCoV preferentially targets non-ciliated and among them the non-mucus-producing cells. Aminopeptidase N (APN), the cellular receptor for PRCoV was also more abundantly expressed on this type of cell suggesting that APN is a determinant of the cell tropism. Interestingly, differentiation-dependent differences were found both in the expression of pAPN and the susceptibility to PRCoV infection. Cells in an early differentiation stage express higher levels of pAPN and are more susceptible to infection by PRCoV than are well-differentiated cells. A difference in the susceptibility to infection was also detected when tracheal and bronchial cells were compared. The increased susceptibility to infection of bronchial epithelial cells was, however, not due to an increased abundance of APN on the cell surface. Our data reveal a complex pattern of infection in porcine differentiated airway epithelial cells that could not be elucidated with immortalized cell lines. The results are expected to have relevance also for the analysis of other respiratory viruses.

show abstract

Comparison of mono- and co-infection by swine influenza A viruses and porcine respiratory coronavirus in porcine precision-cut lung slices

Cited by 13 publications

References 32 publications

Coinfections and their molecular consequences in the porcine respiratory tract

Coinfections and their molecular consequences in the porcine respiratory tract

Cross-Species Genome-Wide Analysis Reveals Molecular and Functional Diversity of the Unconventional Interferon-ω Subtype

The Cell Tropism of Porcine Respiratory Coronavirus for Airway Epithelial Cells Is Determined by the Expression of Porcine Aminopeptidase N

Contact Info

Product

Resources

About