Comparison of molecular techniques for the detection of Toxoplasma gondii in raw bovine milk from small rural properties in Brazil

Manzini, Suzane; Bertozzo, Thainá Valente; Aires, Isabella Neves; Rodrigues, Nássarah Jabur Lot; Bertolini, Amanda Bezerra; Alexandrino, Marcela; Steinle, Jackieline Sampaio; de Melo, Renata Pimentel Bandeira; Mota, Rinaldo Aparecido; de Medeiros, Maria Izabel Merino; Richini-Pereira, Virgínia Bodelão; Curci, Vera Cláudia Lorenzetti Magalhães; Lucheis, Simone Baldini

doi:10.1016/j.ijfoodmicro.2023.110466

Cited by 1 publication

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“…Furthermore, the fact that T. gondii circulates in an environment such as the dairy region is relevant, since Manzini et al reported having found T. gondii in 17.65 % of milk tanks in Brazil by molecular techniques. (31) In addition, Dubey et al reported that toxoplasmosis was the cause of five outbreaks attributed to food poisoning. (32) In addition to the presence of susceptible species, Alvarado-Esquivel et al noted that environmental factors such as altitude, temperature, and mean annual precipitation of 1 266-1 650 mm influence the frequency of parasitosis.…”

Section: Discussionmentioning

confidence: 99%

Toxoplasmosis in cats from a dairy-producing region in Hidalgo, Mexico

Ramirez-Perez,

Rico Torres,

Valenzuela Moreno

et al. 2024

Vet Mex OA

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Considering that the ingestion of cat feces containing Toxoplasma gondii oocysts is a major transmission route for toxoplasmosis, this work is aimed to assess the presence of T. gondii in cats dwelling in dairy production facilities at the Tizayuca Agro-Industrial Complex (CAIT, by its acronym in Spanish) in Hidalgo, Mexico. Blood samples from stray and owned cats were assayed by indirect ELISA, and samples from various tissues were collected at post-mortem examination of positive cats for DNA extraction, PCR, histopathologic studies, and immunohistochemistry. A questionnaire was applied in each dairy production unit to estimate cat ownership. From 70 animals studied (22 stray and 48 owned), 12 (17.1 %) were seropositive (7 stray and 5 owned). No differences were found for age or sex according to the questionnaires, but ingesting raw food was a risk factor (P < 0.01). Of the 12 positive cats, 5 were euthanized; of these, five tissue samples from 4 cats were positive for the B1 and 529 bp repetitive sequences by qPCR and conventional PCR. No macro- or microscopic lesions suggestive of T. gondii infection were found in cats.

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Section: Discussionmentioning

confidence: 99%