Comparison of modified Petroff′s and N-acetyl-L-cysteine-sodium hydroxide methods for sputum decontamination in tertiary care hospital in India

Sharma, Mukesh; Misra, Rabindra Nath; Gandham, Nageswari; Jadhav, Savita; Angadi, Kalpana; Wilson, Vivian J.

doi:10.4103/0975-2870.103323

Cited by 14 publications

(14 citation statements)

References 2 publications

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“…Also, smear positive culture-negative specimens can be resulted from Laboratory errors, prolonged specimen decontamination, shortened incubation times of culture, cross-contamination of smears, using water or stains contaminated with acid-fast organisms or obtaining samples from patients under antimycobacterial therapy (Dunlap et al, 2000). Sharma et al (2012) reported that culture positivity obtained by modified Petroff's method (46.7%) were less Male 3 6 9 18 7 14 1 2 20 40 Female 10 20 16 32 2 4 2 4 30 60 Total 13 26 25 50 9 18 3 6 50 100 *Percentage was correlated to the total number of tuberculosis patients.…”

Section: Resultsmentioning

confidence: 97%

Sociodemographic, microbial and phylogentic studies of Mycobacterium tuberculosis cases diagnosed in El-Minia governorate, Egypt

Yazılıtaş¹,

Rehab²,

Gamal³

et al. 2016

Afr. J. Microbiol. Res.

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Egypt is considered one of the high-burden countries in WHO's Eastern Mediterranean region. This study determined the sociodemographic, microbial and phylogentic characteristics for Mycobacterium tuberculosis cases diagnosed in El-Minia governorate in upper Egypt. Fifty clinical samples were collected (male-to-female sex ratio 1.5 and 50% within the 21-to 40-years old) with complete history of patients; samples were cultured and identified by biochemical tests. DNA was extracted and tested for plc gene. Seven isolates were subjected to sequencing analysis of the amplified plc fragment and a phylogentic tree for these strains was formed. Out of 50 samples, 24 samples were culture positive, all grown isolates were identified as M. tuberculosis according to physical characters, rate of growth, biochemical tests. Direct polymerase chain reaction (PCR) method based on the amplification of 350 bp region of plc gene was used for the detection of M. tuberculosis, to differentiate it from Mycobacterium bovis in clinical samples. It was found that all isolates were positive for plc gene. Direct sequencing of 350 bp region of plc gene revealed many substitution mutations along the entire sequenced fragment. The main cause for such condition may rely on inadequate treatment regimen. The phylogenetic tree of the seven sequenced isolates were classified into three distinct nodes. We concluded that all cases were positive for M. tuberculosis and most of them were from rural area. There was different substitution mutations in the plc gene which may be due to the intermittent treatment regimens that made the tested strains under stress resulting in mutation.

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Section: Resultsmentioning

confidence: 97%

Sociodemographic, microbial and phylogentic studies of Mycobacterium tuberculosis cases diagnosed in El-Minia governorate, Egypt

Yazılıtaş¹,

Rehab²,

Gamal³

et al. 2016

Afr. J. Microbiol. Res.

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“…The concentration of chemical substance of the decontaminant and time contact to bacilli is a crucial factor for Mycobacterium tuberculosis' recovery. 2,10,13 The higher concentration the more toxic to the bacilli's cells, whatever the chemical substance used to decontaminate, it is still have probability to kill a number of TB bacilli. 2,14,15 As a number of bacilliin (+1) AFB is lower than (+2) and (+3) and the bleach concentration and time contact doesn't change, it can make the recovery capability in (+1) AFB is lower too.…”

Section: Resultsmentioning

confidence: 99%

“…6,9 NALC is a good agent as mucolytic, but not as decontaminant, this factor could lead the capability of NALC-NaOH in recovery rate is weaker than Bleach NaOH NALC Bleach Oxalic bleach or NaOH and mask the growth of Mycobacterium tuberculosis. 2,6,10 Based on the number of contamination rate, 5% oxalic acid that had low number of positivity rate was also had a great number in contamination. The contamination that happened in oxalic group, could be because oxalic acid is a poor decontaminant for fungi and Gram positive bacteria as both are abundant flora in oropharyngeal.…”

Section: Resultsmentioning

confidence: 99%

The Evaluations of Bleach as Decontaminant Solution to Promote The Positivity Rate of Mycobacterium Tuberculosis Culture for Sputum Specimen

Suwarsono¹,

Sjahrurachman²,

Karuniawati³

2017

Proceedings of the 1st International Integrative Conference on Health, Life and Social Sciences (ICHLaS 2017)

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Abstract-Background: Tuberculosis (TB) is still worldwide health problems. Sputum is contaminated by normal flora, that make its should be decontaminated prior to culture. Bleach known as a potent disinfectant, easy to access because it is commercially available, could be the alternative solution as decontaminant in TB culture. Aims Assessing the capability of bleach to promote the positivity rate in TB culture compared by others decontaminant solution. Method We use 35 sputum samples with volume 3-5 ml. The research was conducted at TB laboratory of Faculty of Medicine University of Indonesia, by using 35 samples sputum with positive AFB, 3-5 ml. Each sample was divided into 4 groups that were decontaminated by different methods. The methods are 4%NaOH, 2%NALC-NaOH, 5% oxalic acid and 1% bleach. 1% bleach was prepared from commercially bleach. The positive culture was validated using MPT 64. The culture positivity rate was compared based on its AFB score. Result Bleach had the same 100% positivity rate as the 4% NaOH and NALC for (+2) and (+3) AFB. However, its became smaller rate for the (+1) AFB group, which was 88% for bleach and 96% for NALC and 100% for NaOH. Oxalic acid had the smallest positivity rate in all AFB score compared to the others. Conclusion The positivity rate of the bleach as same as 4% NaOH and NALC for AFB score higher than (+2)

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“…All specimens were handled in a negative pressure environment as per the international standards for biosafety and infection control for MTB. For three consecutive mornings sputum samples from each patient were collected in properly labeled screw cap disposable plastic bottles and were decontaminated using N-acetyl-L-cysteine-sodium citrate-sodium-hydroxide (NALC-NaOH) procedure [12]. Sputum samples were processed and stained for Acid…”

Section: Collection Of Mtb Isolatesmentioning

confidence: 99%

Drug Resistance Pattern in the Clinical Isolates of Pulmonary Tuberculosis

Narain¹,

Verma²,

Srivastava³

et al. 2019

Act Scie Micro

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Multidrug-resistant tuberculosis (MDR-TB) is a major public health threat, especially in a country like India, which carries the highest global burden of tuberculosis (TB). TB is caused by Mycobacterium tuberculosis (MTB); the bacteria possess multiple mechanisms that provide it both intrinsic and acquired resistance to current anti-TB drugs. In the current treatment regimen followed for TB, rifampicin and isoniazid are the major drugs given in the first line of treatment. Fluoroquinolones and aminoglycosides are the second line drugs given in case the MTB is resistant to first line drugs. Acquired resistance is mainly due the exposure of MTB to suboptimal concentration of drugs, either due to inadequate treatment given by the medical practitioner or due to the discontinuous treatment regimen followed by patients. Treating drug susceptible TB requires a treatment regimen of almost 6 months which increases significantly from 9 months to 24 months in case of drug resistant cases. In the study conducted we determined the resistance patterns to first and second line anti-TB drugs and the frequency of MDR-TB. Contemplating the drug resistance pattern of MTB will help understanding the epidemiological parameters involved.

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Comparison of modified Petroff′s and N-acetyl-L-cysteine-sodium hydroxide methods for sputum decontamination in tertiary care hospital in India

Cited by 14 publications

References 2 publications

Sociodemographic, microbial and phylogentic studies of Mycobacterium tuberculosis cases diagnosed in El-Minia governorate, Egypt

Sociodemographic, microbial and phylogentic studies of Mycobacterium tuberculosis cases diagnosed in El-Minia governorate, Egypt

The Evaluations of Bleach as Decontaminant Solution to Promote The Positivity Rate of Mycobacterium Tuberculosis Culture for Sputum Specimen

Drug Resistance Pattern in the Clinical Isolates of Pulmonary Tuberculosis

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