Comparison of Mobile Phases and Detection Reagents for the Separation of Triacylglycerols by Silica Gel, Argentation, and Reversed-Phase Thin Layer Chromatography

Masterson, Christine; Fried, Bernard; Sherma, Joseph

doi:10.1080/10826079208016363

Cited by 11 publications

(2 citation statements)

References 8 publications

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“…In this way, selectivity is achieved with respect to the number, position, and geometry of double bonds. This property is used to separate fatty acid derivatives (220,221), lipids (222)(223)(224), and steroids (225)(226)(227).…”

Section: Rabelmentioning

confidence: 99%

Sorbents and Precoated Layers in Thin-Layer Chromatography

Rabel¹

2003

Handbook of Thin-Layer Chromatography

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Section: Rabelmentioning

confidence: 99%

Sorbents and Precoated Layers in Thin-Layer Chromatography

Rabel¹

2003

Handbook of Thin-Layer Chromatography

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“…Silica gel was impregnated with silver nitrate or chloroplatinic acid for the separation of terpenoids ( 010) and with silver nitrate for polyunsaturated fatty acids (Oil), and RP-TLC in the presence of silver ions was used to separate diacylglycerols (012). Comparative studies were made of various mobile phases and detection reagents for the TLC of triacylglycerols on silica gel, argentation, and RP plates (013). Hydroxylated conjugated bile acids were separated by HPTLC utilizing instrumental band application and development in a twin-trough chamber (014) .…”

mentioning

confidence: 99%

Planar Chromatography

Sherma¹

1994

Anal. Chem.

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Lipid Analyses in Food

Marini¹

2000

Encyclopedia of Analytical Chemistry

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The term lipid encompasses a great host of compounds (e.g. fatty acids (FAs), mono‐, di‐ and triglycerides), that are almost ubiquitously present in foods; their characterization and quantification is then of importance for an analytical chemist. The first step for an accurate analysis is sample preparation: choosing an appropriate solvent or phases can isolate one particular class of lipid from a sample [a good separation of classes can be accomplised by thin‐layer chromatography (TLC)]; obviously, the sample pretreatment will be governed by the determinations to follow. Indeed, nowadays, there is a host of techniques, mainly chromatographic, for lipid analysis. TLC is a rapid way to gather information about the sample (presence of conjugated double and triple bonds, free FAs and of oxygenated FAs), particularly with the development of modified normal phases. For example, silica gel can be impregnated with boric acid, which inhibits isomerization of mono‐ and diglycerides, or it can be treated with silver ions [argentation, for silver ion thin‐layer chromatography (Ag‐TLC)] used for resolution of unsaturated FAs. Gas chromatography (GC) and high‐performance liquid chromatography (HPLC) are the most common techniques actually employed, because of their versatility (many parameters can be changed, e.g. stationary and mobile phases, detector) and to their capacity to be hyphenated (coupled with another measuring device). Particularly, the advent of the light‐scattering detector has allowed greater reproducibility in the HPLC of lipids. Mass spectrometry (MS) is often coupled with GC. However, coupling MS with another MS (MS/MS or tandem MS) is useful for the determination of the structure of long‐chain FAs with improved selectivity and sensitivity. Finally, nuclear magnetic resonance (NMR) is able to identify functional groups, even in mixtures, and is a valid aid for the elucidation of structures with double bonds (DBs) by interpretation of the coupling patterns. Each analytical method relies on appropriate sample preparation (e.g. methylation for the GC analysis of FAs) and a critical approach to evaluate its usefulness.

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Comparison of Mobile Phases and Detection Reagents for the Separation of Triacylglycerols by Silica Gel, Argentation, and Reversed-Phase Thin Layer Chromatography

Cited by 11 publications

References 8 publications

Sorbents and Precoated Layers in Thin-Layer Chromatography

Sorbents and Precoated Layers in Thin-Layer Chromatography

Planar Chromatography

Lipid Analyses in Food

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