Comparison of mitochondrial and cytoplasmic ribosomal proteins in Drosophila

Shannon, M. Frances; Duke, E. J.

doi:10.1016/0305-0491(85)90386-4

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…Mitochondrial ribosomes from fly larvae or mouse hearts were prepared as previously described [23], [46]. Mitochondrial ribosomes were loaded onto 10–30% sucrose gradients and separated by centrifugation overnight.…”

Section: Methodsmentioning

confidence: 99%

MTERF3 Regulates Mitochondrial Ribosome Biogenesis in Invertebrates and Mammals

et al. 2013

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Regulation of mitochondrial DNA (mtDNA) expression is critical for the control of oxidative phosphorylation in response to physiological demand, and this regulation is often impaired in disease and aging. We have previously shown that mitochondrial transcription termination factor 3 (MTERF3) is a key regulator that represses mtDNA transcription in the mouse, but its molecular mode of action has remained elusive. Based on the hypothesis that key regulatory mechanisms for mtDNA expression are conserved in metazoans, we analyzed Mterf3 knockout and knockdown flies. We demonstrate here that decreased expression of MTERF3 not only leads to activation of mtDNA transcription, but also impairs assembly of the large mitochondrial ribosomal subunit. This novel function of MTERF3 in mitochondrial ribosomal biogenesis is conserved in the mouse, thus we identify a novel and unexpected role for MTERF3 in coordinating the crosstalk between transcription and translation for the regulation of mammalian mtDNA gene expression.

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Section: Methodsmentioning

confidence: 99%

MTERF3 Regulates Mitochondrial Ribosome Biogenesis in Invertebrates and Mammals

et al. 2013

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“…Mitochondrial extracts from third-instar larvae were obtained as previously described. The final mitochondrial pellets were washed four times in EDTA buffer (154 mM KCl, 5 mM MOPS, 1 mM EDTA, pH 7.4) to ensure removal of cytoplasmic ribosomes according to ( 44 ). Bradford assays were used to assess protein concentrations.…”

Section: Methodsmentioning

confidence: 99%

Drosophila melanogaster LRPPRC2 is involved in coordination of mitochondrial translation

Baggio

Bratić

Mourier

et al. 2014

Nucleic Acids Research

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Members of the pentatricopeptide repeat domain (PPR) protein family bind RNA and are important for post-transcriptional control of organelle gene expression in unicellular eukaryotes, metazoans and plants. They also have a role in human pathology, as mutations in the leucine-rich PPR-containing (LRPPRC) gene cause severe neurodegeneration. We have previously shown that the mammalian LRPPRC protein and its Drosophila melanogaster homolog DmLRPPRC1 (also known as bicoid stability factor) are necessary for mitochondrial translation by controlling stability and polyadenylation of mRNAs. We here report characterization of DmLRPPRC2, a second fruit fly homolog of LRPPRC, and show that it has a predominant mitochondrial localization and interacts with a stem-loop interacting RNA binding protein (DmSLIRP2). Ubiquitous downregulation of DmLrpprc2 expression causes respiratory chain dysfunction, developmental delay and shortened lifespan. Unexpectedly, decreased DmLRPPRC2 expression does not globally affect steady-state levels or polyadenylation of mitochondrial transcripts. However, some mitochondrial transcripts abnormally associate with the mitochondrial ribosomes and some products are dramatically overproduced and other ones decreased, which, in turn, results in severe deficiency of respiratory chain complexes. The function of DmLRPPRC2 thus seems to be to ensure that mitochondrial transcripts are presented to the mitochondrial ribosomes in an orderly fashion to avoid poorly coordinated translation.

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Nucleic Acids and Nuclear Proteins

Urich

1994

Comparative Animal Biochemistry

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Comparison of mitochondrial and cytoplasmic ribosomal proteins in Drosophila

Cited by 4 publications

References 12 publications

MTERF3 Regulates Mitochondrial Ribosome Biogenesis in Invertebrates and Mammals

MTERF3 Regulates Mitochondrial Ribosome Biogenesis in Invertebrates and Mammals

Drosophila melanogaster LRPPRC2 is involved in coordination of mitochondrial translation

Nucleic Acids and Nuclear Proteins

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