Comparison of milk-ELISA and serum-ELISA for the diagnosis of Brucella melitensis infection in sheep

Chand, Puran; Rajpurohit, B. S.; Malhotra, Ashim; Poonia, J. S.

doi:10.1016/j.vetmic.2005.04.006

Cited by 21 publications

(11 citation statements)

References 17 publications

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“…The LPS-based i-ELISA and c-ELISA show high specificity and sensitivity and are considered to be the most effective methods for the diagnosis of brucellosis (8,23). In our study, the Brucellainfected animals could be tested positive for brucellosis during 2 to 4 weeks postinfection; the anti-LPS antibodies persisted for nearly 42 weeks postinfection in the infected animals.…”

Section: Discussionmentioning

confidence: 57%

Limitations of the BP26 Protein-Based Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis

Xin

Yang

Wang

et al. 2013

Clin Vaccine Immunol

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dBrucellosis is a serious zoonosis that occurs worldwide, and its diagnosis is typically based on the detection of antibodies against Brucella lipopolysaccharide (LPS). However, the specificity of the LPS-based test is compromised by cross-reactivity with Escherichia coli O157:H7 and Yersinia enterocolitica O:9. Also, diagnosis based on the LPS test cannot differentiate between vaccinated and infected individuals. The detection of the 26-kDa cytosoluble protein (BP26) antibody is considered an alternative that circumvents these drawbacks because it is exclusively expressed by infectious Brucella. A BP26-based enzyme-linked immunosorbent assay (ELISA) has been tried for the diagnosis of Brucella-infected animals and humans, but a few results showed that BP26 couldn't react with all Brucella-positive sera. In order to explore whether different animals could produce antibodies against BP26 after being infected with various Brucella species, we infected sheep, goats, and beef cattle with common virulent reference Brucella species. All sera were collected from the experimental animals and tested using both LPS-based ELISAs and BP26-based ELISAs. The results showed that all Brucella-infected individuals could produce high levels of antibodies against LPS, but only B. melitensis 16M-and B. melitensis M28-infected sheep and B. melitensis 16M-and B. abortus 2308-infected goats could produce antibodies against BP26. Therefore, we concluded that the BP26-based indirect ELISA (i-ELISA) showed both Brucella species and host specificity, which obviously limits its reliability as a substitute for the traditional LPS-based ELISA for the detection of brucellosis. Brucella is a Gram-negative, facultative intracellular bacterial pathogen that causes one of the world's most widely spread zoonotic infections, including infectious abortion in animals and Malta fever in humans (1, 2). Brucella species include Brucella melitensis (natural host: goat), Brucella abortus (cattle), Brucella ovis (sheep), Brucella suis (swine), Brucella canis (dogs), and Brucella neotomae (desert rats) as well as some strains that infect marine mammals (3). Besides their natural hosts, most Brucella species also infect other animals. B. melitensis and B. abortus are considered to be major health threats because of their highly infectious nature and worldwide occurrence (3-5). Control of brucellosis depends on reliable diagnostic methods.The lipopolysaccharide (LPS) of smooth Brucella species is an antigen of strong reactivity and can elicit a long-lasting serological response in both vaccinated and infected animals (6, 7). Serological tests based on the detection of antibodies against lipopolysaccharide (LPS), like the Rose Bengal plate agglutination test, the complement fixation test, the fluorescence polarization assay, and enzyme-linked immunosorbent assays (ELISAs) display satisfying specificity and sensitivity and therefore are widely used for the diagnosis of brucellosis. Among these serological tests, ELISAs showed the highest sensitivity and specifi...

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Section: Discussionmentioning

confidence: 57%

Limitations of the BP26 Protein-Based Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis

Xin

Yang

Wang

et al. 2013

Clin Vaccine Immunol

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“…compared to check the level of antibody in ewe's milk and serum, where milk ELISA was confirmed as superior than serum ELISA (Chand et al, 2005). Milk i-ELISA detects IgG antibodies (Rahman et al, 2013) and the primary advantage of Milk i-ELISA is screening from bulk milk samples, but the individual samples can also be tested (Funk et al, 2005).…”

Section: Research Articlementioning

confidence: 99%

Milk Indirect-ELISA and Milk Ring Test for Screening of Brucellosis in Buffaloes, Goats and Bulk Tank Milk Samples Collected from Two Districts of Punjab, Pakistan

Khan¹

2018

PVJ

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Bovine and caprine brucellosis caused by Brucella abortus and Brucella melitensis, are serious zoonotic problem. In this study, the prevalence and risk factors of brucellosis were studied at herd level in two Districts of Punjab. A total of 300 milk samples from buffaloes, goats and bulk tank milk (BTM) were analyzed through milk ring test (MRT), and through a commercially available Milk Indirect ELISA (i-ELISA) kit detecting both B. abortus and B. melitensis antibodies. Higher prevalence was found in goats (76%) followed by BTM samples (42%) and buffalo samples (15%) as tested though Milk i-ELISA. Diagnostic sensitivity (DSe) and specificity (DSp) of MRT was evaluated considering Milk i-ELISA as gold standard test for diagnosis of B. abortus and B. melitensis infection. DSe and DSp of MRT for buffalo milk samples was found as 78.9% and 100% respectively. While for goat samples DSe and DSp of MRT was found as 51.7% and 100% respectively. It is concluded that, MRT is less effective for diagnosis of Brucella antibodies in goat milk as compared to buffalo. Adult age, self-reared animals and larger herd size were found as potential risk factors for the spread of brucellosis in both goat and buffalo. Whereas, use of natural insemination method was found as a risk factor in goat population only and artificial insemination in buffalo only. With the advent of corporate dairy sector in the Pakistan, it is highly recommended that routine Brucella screening should be done using Milk i-ELISA on pooled milk samples.

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“…In dairy herds, the level of antibodies to Bovine viral diarrhoea virus , Brucella melitensis or Neospora caninum in bulk tank milk (BTM), measured by ELISA, has been reported as an inexpensive and valuable tool to assess the within‐herd prevalence of seropositive cows in a herd (Beaudeau et al, 2001; Chand et al, 2005; Frössling et al, 2006). ELISA tests are currently applied to BTM to identify and monitor herds in the control and eradication programmes for BVDV (Niskanen et al, 1991; Lindberg and Alenius, 1999; Joly et al., 2005).…”

Section: Introductionmentioning

confidence: 99%

Assessing the Within‐herd Prevalence of Coxiella burnetii Milk‐shedder Cows using a Real‐time PCR Applied to Bulk Tank Milk

Guatteo

Beaudeau

Joly³

et al. 2007

Zoonoses and Public Health

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Thirty-seven bulk tank milk (BTM) and individual milk samples of all contributing cows were tested for Coxiella burnetii detection by a real-time PCR assay and used to assess the relationship between the BTM PCR-response and (i) the within-herd prevalence of milk-shedder cows and (ii) the proportion of heavy milk-shedder cows. The within-herd prevalence of milk-shedder cows (i) was found to be significantly higher in herds with a positive BTM and (ii) increased significantly with the estimated titre in Coxiella burnetii obtained in positive BTM. The proportion of heavy milk-shedder cows among the milk-shedder cows increased significantly with an increased estimated titre in Coxiella burnetii in positive BTM. Therefore, a real-time PCR assay applied to BTM samples collected repeatedly over time appears to be a valuable tool to assess on a larger scale the status of herds towards Coxiella shedding, and to evaluate the efficiency of control actions aimed at controlling and/or preventing Coxiella shedding in dairy herds.

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Comparison of milk-ELISA and serum-ELISA for the diagnosis of Brucella melitensis infection in sheep

Cited by 21 publications

References 17 publications

Limitations of the BP26 Protein-Based Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis

Limitations of the BP26 Protein-Based Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis

Milk Indirect-ELISA and Milk Ring Test for Screening of Brucellosis in Buffaloes, Goats and Bulk Tank Milk Samples Collected from Two Districts of Punjab, Pakistan

Assessing the Within‐herd Prevalence of Coxiella burnetii Milk‐shedder Cows using a Real‐time PCR Applied to Bulk Tank Milk

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