Comparison of Microscopic Agglutination, Indirect Haemagglutination and Complement-Fixation Tests in Rabbit and Buffalo-Calf Hyperimmune Sera for Detection of Leptospiral Antibodies

Palit, A.; Sharma, Gautam

doi:10.1016/s0007-1935(17)37632-7

Cited by 9 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The indirect haemagglutination (HA) test was performed essentially as described by Chang and McComb (1954) and Palit and Sharma (1971). The optimum dilution of antigen required for maximum sensitization of the red cells for the HA test was determined by the conventional checker board titration (Table I).…”

Section: Indirect Haemagglutination (Ha) Testmentioning

confidence: 99%

Genus-specific leptospiral antigen and its possible use in laboratory diagnosis

Palit

Gulasekharam

1973

J Clin Pathol

Self Cite

View full text Add to dashboard Cite

SYNOPSIS Sheep red cells, sensitized with a genus-specific leptospiral substance extracted from a water leptospira, were preserved by freeze drying. The serological reactivity of the preparation was tested against a total of 41 sera from proven cases of human leptospirosis and 27 from proven cases of animal leptospirosis. The sensitized, freeze-dried red cells appeared to be a sensitive reagent for the detection of leptospiral antibodies by haemagglutination. The use of this reagent as a screening test for leptospiral diagnosis is discussed. In the reported haemagglutination test on human and rabbit antisera the findings suggested that only IgM (19S) antibodies took part in the reaction.

show abstract

Section: Indirect Haemagglutination (Ha) Testmentioning

confidence: 99%

Genus-specific leptospiral antigen and its possible use in laboratory diagnosis

Palit

Gulasekharam

1973

J Clin Pathol

Self Cite

View full text Add to dashboard Cite

show abstract

“…), immunodiffusion in agar gel, the indirect or passive haemagglutination (h.a.) tests and the titration of 90 % ethanol extract for the determination of the optimum erythrocyte sensitizing dilution have been described elsewhere (Palit & Gulasekharam, 1973;Palit & Sharma, 1971).…”

Section: Introductionmentioning

confidence: 99%

Further Studies on Leptospiral Genus-specific Antigen: Its Ultrastructure and Immunochemistry

Palit

Hamilton

Gulasekharam

1974

Journal of General Microbiology

Self Cite

View full text Add to dashboard Cite

SUMMARYFifty per cent ethanol treatment removed most of the outer envelope of serotype bzjlexa, strain Patoc I. The trilaminar outer envelope was recovered from the 5 0 % ethanol supernatant by raising the ethanol concentration to 90% (v/v).Five and even seven layers in the outer envelopes were seen. The 90 % ethanol precipitate contained the erythrocyte-sensitizing substance (ESS). An anti-ESS serum was reactive in leptospiral agglutination, haemagglutination and in Ouchterlony's double diffusion tests. Electron microscopic examination revealed the presence of stripping and agglutination of stripped or unstripped outer envelopes of healthy Patoc I cells in the presence of anti-ESS serum. The 90 % ethanol precipitate of Patoc I organisms contained polysaccharide, lipid, protein, amino sugars and 16 different amino acids. The suggestion that the genus-specific leptospiral erythrocyte-sensitizing substance is, or is associated with, the outer envelope of strain Patoc I is discussed.

show abstract

“…The results test, especially for establishing the infecting serotype. in table V show that there is a type I error (false The broad cross reactivity of rabbit immune sera has been demonstrated in complement fixation tests (Palit and Sharma, 1971;Robertson and Boulanger, 1963), haemagglutination tests (Palit and Sha-rma, 1971), haemagglutination-lysis tests (Cox, 1955), and in the macroscopic slide test (Galton et al, 1958), while bovine sera show very little cross-reactivity. Cross reactions have been observed in most leptospiral serological tests when human sera have been examined, the frequent reaction with serotype patoc leading to the development of many genus-specific antigens (Combiescu et al, 1958;Cox, 1957;Elian and Nicoaia, 1964;Palit and Gulasekharam, 1973).…”

Section: Rabbit Antiseramentioning

confidence: 98%

A card test for the serodiagnosis of human leptospirosis.

Bragger¹,

Adler²

1976

J Clin Pathol

View full text Add to dashboard Cite

SYNOPSIS A macroscopic agglutination test for detecting leptospiral antibodies in human sera is described. The test utilizes a stained preparation of non-pathogenic leptospires and is performed on the Brewer diagnostic cards used for bovine brucellosis screening. The agglutination of the stained antigen is more easily observed than the current macroscopic slide test using unstained leptospiral antigens. The non-pathogenic serotype patoc is agglutinated by sera from humans infected with serotypes pomona, hardjo, ballum, and copenhageni with a sensitivity of 94 % in comparison with the microscopic agglutination test.

show abstract

Comparison of Microscopic Agglutination, Indirect Haemagglutination and Complement-Fixation Tests in Rabbit and Buffalo-Calf Hyperimmune Sera for Detection of Leptospiral Antibodies

Cited by 9 publications

References 6 publications

Genus-specific leptospiral antigen and its possible use in laboratory diagnosis

Genus-specific leptospiral antigen and its possible use in laboratory diagnosis

Further Studies on Leptospiral Genus-specific Antigen: Its Ultrastructure and Immunochemistry

A card test for the serodiagnosis of human leptospirosis.

Contact Info

Product

Resources

About