Comparison of microsatellite DNA diversity among commercial queen breeder stocks of Italian honey bees in the United States and Italy

Bourgeois, Lelania; Sylvester, Allen; Danka, Robert G.; Rinderer, Thomas E.

doi:10.1080/00218839.2008.11101432

Cited by 13 publications

(11 citation statements)

References 19 publications

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“…This may be an artifact of marker selection. The markers selected for this study differ from those used in previous studies assessing genetic diversity for both Russian and Italian honey bees (Bourgeois et al 2008, Bourgeois andRinderer 2009). The markers in the current study were selected for homogeneity within each grouping (i.e., Russian or non-Russian).…”

Section: Discussionmentioning

confidence: 99%

Genetic Stock Identification of Russian Honey Bees

Bourgeois

Sheppard

Sylvester

et al. 2010

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A genetic stock certification assay was developed to distinguish Russian honey bees from other European (Apis mellifera L.) stocks that are commercially produced in the United States. In total, 11 microsatellite and five single-nucleotide polymorphism loci were used. Loci were selected for relatively high levels of homogeneity within each group and for differences in allele frequencies between groups. A baseline sample consisted of the 18 lines of Russian honey bees released to the Russian Bee Breeders Association and bees from 34 queen breeders representing commercially produced European honey bee stocks. Suitability tests of the baseline sample pool showed high levels of accuracy. The probability of correct assignment was 94.2% for non-Russian bees and 93.3% for Russian bees. A neighbor-joining phenogram representing genetic distance data showed clear distinction of Russian and non-Russian honey bee stocks. Furthermore, a test of appropriate sample size showed a sample of eight bees per colony maximizes accuracy and consistency of the results. An additional 34 samples were tested as blind samples (origin unknown to those collecting data) to determine accuracy of individual assignment tests. Only one of these samples was incorrectly assigned. The 18 current breeding lines were represented among the 2009 blind sampling, demonstrating temporal stability of the genetic stock identification assay. The certification assay will be used through services provided by a service laboratory, by the Russian Bee Breeders Association to genetically certify their stock. The genetic certification will be used in conjunction with continued selection for favorable traits, such as honey production and varroa and tracheal mite resistance.

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Section: Discussionmentioning

confidence: 99%

Genetic Stock Identification of Russian Honey Bees

Bourgeois

Sheppard

Sylvester

et al. 2010

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“…It is known that the anion products of dissolved salts individually affect the aggregation or precipitation of proteins from solutions such that the efficacies can be described by the Hofmeister series (Zhang & Cremer, ; Kunz, ). Although many DNA extraction protocols (Bourgeois et al., , ; Bourgeois & Rinderer, ) and kits use acetate salts (potassium or ammonium), we chose to focus on sodium chloride due to its availability and universal use in a variety of laboratories. Chloride anion is considered a marginally functional salting‐out factor, somewhat less effective than acetate on the Hofmeister series.…”

Section: Resultsmentioning

confidence: 99%

“…Traditional phenol‐chloroform extractions require handling, storage, and disposal of hazardous organic solvents. Honey bee DNA has recently been effectively extracted and purified by homogenization and utilizing proteinase K and ‘salting‐out’ methods to remove proteins (Bourgeois et al., , ; Bourgeois & Rinderer, ). However, we developed a simple, cost‐effective, and fast method using only sodium chloride and sodium dodecylsulfate (SDS) to extract clean DNA directly useable for PCR without additional dilution as is typically required.…”

Section: Introductionmentioning

confidence: 99%

An economical and effective high‐throughput DNA extraction protocol for molecular marker analysis in honeybees

Holloway

Tarver

Rinderer

2013

Entomologia Exp Applicata

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“…Queens of one group can be produced and mated simultaneously. This mating scheme has resulted in a stock that retains good genetic diversity among groups and lines (Bourgeois et al, 2008). Also, allelic frequency differences at molecular loci enable RHBs to be distinguished from other commercial stocks with very high accuracy (Bourgeois and Rinderer, 2009).…”

Section: Development Of a Closed Breeding Population Of Rhbmentioning

confidence: 99%

Breeding for resistance toVarroa destructorin North America

et al. 2010

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Breeding for resistance to Varroa destructor in North America provides the long-term solution to the economic troubles the mite brings. This review reports the development of two breeding successes that have produced honey bees of commercial quality that do not require pesticide treatment to control Varroa, highlights other traits that could be combined to increase resistance and examines the potential uses of marker-assisted selection (MAS) for breeding for Varroa resistance. Breeding work continues with these stocks to enhance their commercial utility. This work requires knowledge of the mechanisms of resistance that can be further developed or improved in selected stocks and studied with molecular techniques as a prelude to MAS.Varroa resistance / breeding program / Russian honey bees / Varroa-sensitive hygiene / marker-assisted selection

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Comparison of microsatellite DNA diversity among commercial queen breeder stocks of Italian honey bees in the United States and Italy

Cited by 13 publications

References 19 publications

Genetic Stock Identification of Russian Honey Bees

Genetic Stock Identification of Russian Honey Bees

An economical and effective high‐throughput DNA extraction protocol for molecular marker analysis in honeybees

Breeding for resistance toVarroa destructorin North America

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