Comparison of microbial numbers in soils by using various culture media and temperatures

Vieira, Francisco Cleber Sousa; Nahas, Ely

doi:10.1016/j.micres.2005.01.004

Cited by 159 publications

(96 citation statements)

References 33 publications

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“…A metodologia de cultivo direto utilizada para caracterização quantitativa da presença de fungos no solo apresentou-se com limitações quando comparada com as técnicas moleculares (Bridge & Spooner, 2001;Kirk et al, 2004;Vieira & Nahas, 2005 …”

Section: áRea/terçosunclassified

Micobiota do solo de uma área de duna na Restinga da Marambaia, Rio de Janeiro, RJ

Fraga¹,

Pereira²,

Souza³

2010

Floram

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RESUMOEste trabalho avaliou a micobiota do solo de uma duna nos terços inferior, médio e superior de encosta, localizada na Restinga da Marambaia, RJ. A área de estudo com aproximadamente 500 m 2 está dividida em três terços, com a vegetação variando de arbustiva a arbórea. Foi delimitada uma gleba de dimensões de 50 x 50 m, onde foram abertas cinco minitrincheiras até a profundidade de 10 cm para coleta, sendo coletadas três amostras compostas formadas a partir da reunião de cinco amostras simples para análise do solo e caracterização da micobiota. A fertilidade do solo indicou que o terço inferior apresentou maiores teores de alumínio e menores de nutrientes. As unidades formadoras de colônias de fungos variaram de 4,6 x 10 3 a 9 x 10 4 ufc.g -1 . Quarenta e nove fungos, num total de 85 isolados, pertencentes a 13 gêneros e 32 espécies foram identificados. Os gêneros mais frequentes foram Penicillium, Aspergillus e Trichoderma. A maior riqueza ocorreu no terço médio, porém uma espécie (Trichoderma pseudokoningi) foi comum entre as áreas. Este trabalho sugere que a Restinga da Marambaia apresenta grande diversidade fúngica.Palavras-chave: restinga, micobiota, solo. Soil Mycobiota in a Dune Area in the Municipality of Restinga da Marambaia, State of Rio de Janeiro, Brazil ABSTRACTThis study was carried out in order to evaluate soil mycobiota in different soil positions (shoulder, back slope, foot slope) in a dune, located in Restinga da Marambaia, RJ. The studied area of approximately 500 m 2 was divided in three parts with vegetation ranging from the shrubs to trees. It was a 50 x 50 m enclosed plot where five 10 cm-deep mini trenches were opened for soil collection. Three composite samples formed by the gathering of five single samples were collected for analysis and characterization of the soil mycobiota. The highest aluminum content and the lowest nutrient content were observed in the foot slope position. The colony unit formation varied from 4.6 x 103 to 9.0 x 104 cuf.g -1 . 49 fungi -in a total of 85 isolated, belonging to 13 genders and 32 species -were found. Penicillium, Aspergillus and Trichoderma were the most frequent genders. The highest richness value was observed in the back slope position. Trichoderma pseudokoningi was the most common species found in the areas. This study implies that Restinga da Marambaia presents a great fungal diversity.

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Section: áRea/terçosunclassified

Micobiota do solo de uma área de duna na Restinga da Marambaia, Rio de Janeiro, RJ

Fraga¹,

Pereira²,

Souza³

2010

Floram

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“…These culturedependent approaches to characterize microbial communities have built-in biases in the isolation of microorganisms. Recent attempts have been made to develop new culture media to maximize the recovery of diverse microbial groups (Davis et al 2005;Vieira and Nahas 2005). This is particularly the case for studies of soil health.…”

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Microbial composition and diversity of an upland red soil under long-term fertilization treatments as revealed by culture-dependent and culture-independent approaches

et al. 2008

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Background, aim, and scope Fertilization is an important agricultural practice for increasing crop yields. In order to maintain the soil sustainability, it is important to monitor the effects of fertilizer applications on the shifts of soil microorganisms, which control the cycling of many nutrients in the soil. Here, culture-dependent and cultureindependent approaches were used to analyze the soil bacterial and fungal quantities and community structure under seven fertilization treatments, including Control, Manure, Return (harvested peanut straw was returned to the plot), and chemical fertilizers of NPK, NP, NK, and PK. The objective of this study was to examine the effects on soil microbial composition and diversity of long-term organic and chemical fertilizer regimes in a Chinese upland red soil. Materials and methods Soil samples were collected from a long-term experiment station at Yingtan (28°15′N, 116°55′E), Jiangxi Province of China. The soil samples (0-20 cm) from four individual plots per treatment were collected. The total numbers of culturable bacteria and fungi were determined as colony forming units (CFUs) and selected colonies were identified on agar plates by dilution plate methods. Moreover, soil DNAs were extracted and bacterial 16S rRNA genes and fungal 18S rRNA genes were polymerase chain reaction amplified, and then analyzed by denaturing gradient gel electrophoresis (DGGE), cloning, and sequencing. Results The organic fertilizers, especially manure, induced the least culturable bacterial CFUs, but the highest bacterial diversity ascertained by DGGE banding patterns. Chemical fertilizers, on the other hand, had less effect on the bacterial composition and diversity, with the NK treatment having the lowest CFUs. For the fungal community, the manure treatment had the largest CFUs but much fewer DGGE bands, also with the NK treatment having the lowest CFUs. The conventional identification of representative bacterial and fungal genera showed that long-term fertilization treatments resulted in differences in soil microbial composition and diversity. In particular, 42.4% of the identified bacterial isolates were classified into members of Arthrobacter. For fungi, Aspergillus, Penicillium, and Mucor were the most prevalent three genera, which accounted for 46.6% of the total identified fungi. The long-term fertilization treatments resulted in different bacterial and fungal compositions ascertained by the culture-dependent and also the culture-independent approaches. Discussion It was evident that more representative fungal genera appeared in organic treatments than other treatments, indicating that culturable fungi were more sensitive to organic than to chemical fertilizers. A very notable finding was that fungal CFUs appeared maximal in organic manure treatments. This was quite different from the

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“…This is regarded to the benefits of plant inoculation with red yeast; microbial population play crucial role in lowering pathogen infection, improving fertilizer efficiency and improve abiotic stress-resistance (Yang et al, 2009;Kim et al, 2011). Also, soil bacteria and fungi effectively contribute in decomposing the organic matter, chemical releasing nutrients and enhancing plant growth (Vieira and Nahas, 2005). F/B ratio is a good marker used to assess the sustainability of the agriculture systems (de Vries et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Influence of Bio-fertilization with the Red Yeast (Phaffia rhodozyma) on Growth, Yield and Inulin Content of Jerusalem artichoke

Moustafa¹,

Haddad²,

Ahmed³

2017

Egypt. J. Exp. Biol. (Bot.)

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ABSTRACT:In a clay loam soil in the Middle Egypt, an experiment was conducted to study the effect of soil inoculation with red yeast (Phaffia rhodozyma) on growth, physiological characteristics and tubers production of Jerusalem artichoke ( Helianthus tuberosus L.). Jerusalem artichoke plants' growth was motivated as a result of the increased microbial activities in the treated soil; also, tubers yield/plant was significantly increased. Soluble, insoluble and total carbohydrates at yield stage were found to decrease in the treated Jerusalem artichoke shoots than in control ones. Also, red yeast significantly enhanced inulin content in tubers of the treated than that of the non-treated Jerusalem artichoke plants. Results also showed that this yeast isolate improved soil properties by increasing t he total count of bacteria, fungi and actinomycetes. Application of red yeast as a bio-fertilizer of a plant promoting microorganisms is promising to enhance plant growth and yield, stimulate translocation of carbohydrates towards tubers and decreased the need for chemical fertilizers resulting in, organic matter with lower costs.

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Comparison of microbial numbers in soils by using various culture media and temperatures

Cited by 159 publications

References 33 publications

Micobiota do solo de uma área de duna na Restinga da Marambaia, Rio de Janeiro, RJ

Micobiota do solo de uma área de duna na Restinga da Marambaia, Rio de Janeiro, RJ

Microbial composition and diversity of an upland red soil under long-term fertilization treatments as revealed by culture-dependent and culture-independent approaches

Influence of Bio-fertilization with the Red Yeast (Phaffia rhodozyma) on Growth, Yield and Inulin Content of Jerusalem artichoke

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