Comparison of Microbial Inhibition and Enzymatic Hydrolysis Rates of Liquid and Solid Fractions Produced from Pretreatment of Biomass with Carbonic Acid and Liquid Hot Water

Yourchisin, Damon M.; Walsum, G. Peter van

doi:10.1007/978-1-59259-837-3_87

Cited by 11 publications

(13 citation statements)

References 27 publications

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“…Feedstock handling and biomass pretreatment were conducted using a method similar to previously reported procedures (Chen et al, 2006;Yourchisin and van Walsum, 2004). All feedstocks were initially ground using a coffee grinder, sieved with a 16-mesh filter and dried at 1058C for 20 h. Approximately 0.8 g dry biomass was then pretreated at a solids concentration of 10 g/L using each of the conditions specified in Table II.…”

Section: Biomass Handling and Pretreatmentmentioning

confidence: 99%

“…Investigated feedstocks (i.e., corn stover, poplar, and pine) were chosen to represent major lignocellulosic biomass sources from agricultural residues, hardwood, and softwood, respectively. Eight pretreatment chemistries were investigated: 0.7% H 2 SO 4 , 0.07% H 2 SO 4 (Chen et al, 2007a;Hsu, 1996;Jacobsen and Wyman, 2001;Lloyd and Wyman, 2005), liquid hot water (van Walsum et al, 1996;Weil et al, 1997;Yourchisin and van Walsum, 2004), neutral buffer solution (Mosier et al, 2005b,c), aqueous ammonia (Kim and Lee, 2005;Kim et al, 2000), lime (Blackman and van Walsum, 2008;Chang et al, 1997Chang et al, , 1998Holtzapple, 2005, 2006), lime with oxygen pressurization (Blackman and van Walsum, 2008;Chang et al, 2001), and wet oxidation (Bjerre et al, 1996;Klinke et al, 2002Klinke et al, , 2003.…”

Section: Introductionmentioning

confidence: 99%

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Effect of varying feedstock–pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates

Sharma

Becker

et al. 2010

Biotech & Bioengineering

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202

135

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A variety of potentially inhibitory degradation products are produced during pretreatment of lignocellulosic biomass. Qualitative and quantitative interrogation of pretreatment hydrolysates is paramount to identifying potential correlations between pretreatment chemistries and microbial inhibition in downstream bioconversion processes. In the present study, corn stover, poplar, and pine feedstocks were pretreated under eight different chemical conditions, which are representative of leading pretreatment processes. Pretreatment processes included: 0.7% H(2)SO(4), 0.07% H(2)SO(4), liquid hot water, neutral buffer solution, aqueous ammonia, lime, lime with oxygen pressurization, and wet oxidation. Forty lignocellulosic degradation products resulting from pretreatment were analyzed using high performance liquid chromatography in combination with UV spectroscopy or tandem mass spectrometry detection (HPLC-PDA-MS/MS) and ion chromatography (IC). Of these compounds, several have been reported to be inhibitory, including furfural, hydroxymethyl furfural, ferulic acid, 3,4-dihydroxybenzaldehyde, syringic acid among others. Formation and accumulation of monitored compounds in hydrolysates is demonstrated to be a function of both the feedstock and pretreatment conditions utilized.

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Section: Biomass Handling and Pretreatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of varying feedstock–pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates

Sharma

Becker

et al. 2010

Biotech & Bioengineering

Self Cite

202

135

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show abstract

“…It was viewed as important to integrate pretreatment, which had been an obstacle to developing a fully integrated HTP pipeline, as seamlessly as possible with an existing multiwell plate method (Decker et al, 2003) that employs robots for evaluation of combinations of enzymes and feedstock. For screening to identify more easily hydrolysable biomass materials, we decided to add enzymes directly to the liquor from pretreatment and to add excess enzyme and perform pretreatment at only 1% (w/w) glucan concentrations to minimize inhibition of enzymes by sugars that could otherwise mask differentiating biomass recalcitrance from enzyme inhibition while still assuring adequate sugar concentrations for analysis (Kumar and Wyman, 2009;Palmqvist et al, 1996;Panagiotou and Olsson, 2007;Sanderso, 1965;Yourchisin and Van Walsum, 2004). We termed this approach that we have demonstrated to work successfully in conventional reactors as co-hydrolysis.…”

Section: Introductionmentioning

confidence: 97%

Engineering of a high‐throughput screening system to identify cellulosic biomass, pretreatments, and enzyme formulations that enhance sugar release

Studer¹,

DeMartini²,

Brethauer³

et al. 2009

Biotech & Bioengineering

106

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The recalcitrance of cellulosic biomass, the only abundant, sustainable feedstock for making liquid fuels, is a primary obstacle to low cost biological processing, and development of more easily converted plants and more effective enzymes would be of great benefit. Because no single parameter describes recalcitrance, superior variants can only be identified by measuring sugar release from plants subjected to pretreatment and enzymatic hydrolysis. However, genetic modifications of plants coupled with molecular engineering of deconstruction proteins and definition of pretreatment conditions create a very large sample set, and previous methods for biomass pretreatment at elevated temperatures and pressures prevented use of a fully integrated high-throughput (HTP) screening pipeline. Herein, we report on the engineering of a novel HTP pretreatment system employing a 96 well-plate format that withstands extreme pretreatment conditions for rapid screening of biomass-enzyme-pretreatment combinations. This includes the development of new approaches to steam heating and water quenching the system that result in much faster heat up and cool down than previously possible and show consistent temperature histories across the multiwell plate. Coupled pretreatment and enzymatic hydrolysis performance of the well plate pretreatment system is shown to be consistent among the many wells in the device and also with performance of conventional tubular reactors.

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“…Because of such characteristic of lignocellulosic biomass, pretreatment is required for efficient enzymatic hydrolysis (Mosier, et al 2005). 3 Numerous biomasses have been studied for biofuel production, such as wheat straw (Alinia, et al 2010), aspen (Yourchisin and Walsum 2004), soybean straw (Wan and Li 2011), and corn stover (Narayanaswamy, et al 2011). The primary lignocellulosic biomass suitable is corn stover, which is regarded as one of the most important source of bioenergy, bioethanol and a few commodity chemicals (Sassner et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Low-Moisture Anhydrous Ammonia (LMAA) Pretreatment of Corn Stover

Yang¹,

Rosentrater²

2014

2014 ASABE Annual International Meeting

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With many environmental benefits, corn-based ethanol has been widely used in recent years. Cellulosic ethanol, however, will require pretreatment to break down lingo-cellulosic structures prior to fermentation. Among all the pretreatment reagents which can be used, ammonia has been shown to be one of the most effective, because it can readily delignify, swell, and preserve the cellulose. Previous work evaluated the effectiveness of the LMAA pretreatment method. The purpose of this study was to determine optimal conditions (i.e. highest glucose yield) using the LMAA pretreatment process. In this experiment, corn stover was prepared with different moisture contents (20%, 50%, 80% wt) and particle sizes (9-30 mesh, 30-144 mesh). Corn stover was ammoniated at 20 psi for 30 minutes. Ammoniated corn stover then was subjected to different incubation times (24h, 75h, and 144h) under different temperatures (20C, 70C, 120C). After that, compositional analyses, including ash content, solids content, structural carbohydrates, and lignin content, were conducted. Enzymatic digestibility tests were also conducted. Results from this study will be used to improve the efficiency of pretreatment processing. Keywords LMAA, Ammonia, Ethanol Disciplines Agriculture | Bioresource and Agricultural EngineeringThis conference proceeding is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/abe_eng_conf/390The authors are solely responsible for the content of this technical presentation. The technical presentation does not necessarily reflect the official position of the American Society of Agricultural and Biological Engineers (ASABE), and its printing and distribution does not constitute an endorsement of views which may be expressed. Technical presentations are not subject to the formal peer review process by ASABE editorial committees; therefore, they are not to be presented as refereed publications. Citation of this work should state that it is from an ASABE meeting paper. EXAMPLE: Author's Last Name, Initials.

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Comparison of Microbial Inhibition and Enzymatic Hydrolysis Rates of Liquid and Solid Fractions Produced from Pretreatment of Biomass with Carbonic Acid and Liquid Hot Water

Cited by 11 publications

References 27 publications

Effect of varying feedstock–pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates

Effect of varying feedstock–pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates

Engineering of a high‐throughput screening system to identify cellulosic biomass, pretreatments, and enzyme formulations that enhance sugar release

Optimization of Low-Moisture Anhydrous Ammonia (LMAA) Pretreatment of Corn Stover

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