Comparison of microbial* gases from the Middle America Trench and Scripps Submarine Canyon: implications for the origin of natural gas

Jenden, Peter D.; Kaplan, I. R.

doi:10.1016/0883-2927(86)90085-5

Cited by 104 publications

(49 citation statements)

References 33 publications

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“…Indeed, it has been reported that pore water in a variety of marine sediments contain micromolar amounts of acetate (44), concentrations that severely restrict the amount of energy available for ATP synthesis, which argues in favor of an essential requirement for the Mrp complex during acetotrophic growth of M. acetivorans in its native environment. M. acetivorans was isolated from a submarine canyon containing sediments with a high fraction of organic material that continually accumulated from the littoral zone (23), which results in an estimated fraction of methanogenesis from acetate of between 50 and 70% based on isotopic analysis (2). Since the pH and salinity were not recorded for the site and the time of isolation, the pH (6.8) and salinity level (0.54 M) tested were based on the published values for optimal growth of M. acetivorans (23).…”

Section: Discussionmentioning

confidence: 99%

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MrpA Functions in Energy Conversion during Acetate-Dependent Growth of Methanosarcina acetivorans

Jasso‐Chávez

Apolinario

Sowers

et al. 2013

Journal of Bacteriology

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bThe role of the multisubunit sodium/proton antiporter (Mrp) of Methanosarcina acetivorans was investigated with a mutant deleted for the gene encoding the MrpA subunit. Antiporter activity was 5-fold greater in acetate-grown versus methanol-grown wild-type cells, consistent with the previously published relative levels of mrp transcript. The rate, final optical density, and dry weight/methane ratio decreased for the mutant versus wild type when cultured with a growth-limiting concentration of acetate. All growth parameters of the mutant or wild type were identical when grown with methanol in medium containing a growthlimiting Na ؉ concentration of 1.04 M. The lag phase, growth rate, and final optical density for growth of the mutant were suboptimal compared to the wild type when cultured with acetate in medium containing either 0.54 or 1.04 M Na ؉ . The addition of 25 mM NaCl to resting cell suspensions stimulated ATP synthesis driven by a potassium diffusion potential. ATP synthesis was greater in wild-type than mutant cells grown with acetate, a trend that held for methanol-grown cells, albeit less pronounced. Both sodium and proton ionophores reduced ATP synthesis in the wild type grown with either substrate. The results indicated that the Mrp complex is essential for efficient ATP synthesis and optimal growth at the low concentrations of acetate encountered in the environment.

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Section: Discussionmentioning

confidence: 99%

“…cetate is the major source of biological methane in both freshwater and marine environments (1,2). Only two genera (Methanosarcina and Methanosaeta) of acetate-utilizing methaneproducing microbes are known, of which Methanosarcina species have been researched to a greater extent.…”

mentioning

confidence: 99%

MrpA Functions in Energy Conversion during Acetate-Dependent Growth of Methanosarcina acetivorans

Jasso‐Chávez

Apolinario

Sowers

et al. 2013

Journal of Bacteriology

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“…2). Bacterial methane formation follows two principal pathways (i.e., CO 2 reduction and fermentation; Schoell, 1980;Jenden and Kaplan, 1986;Martens et al, 1986;Whiticar et al, 1986;Burke et al, 1988). The fermentation processes are usually more important in recent freshwater sediments and swamps, while methane formed by CO 2 reduction is most common in marine sediments (Whiticar et al, 1986).…”

Section: The δmentioning

confidence: 99%

Isotope Compositions of Gases in Sediments from the Chile Continental Margin

Waseda¹,

Didyk²

1995

Proceedings of the Ocean Drilling Program, 141 Scientific Results

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Molecular and isotope measurements of gases from gas pockets at ODP Leg 141 Sites 859, 860, 861, and 863 are reported. The δ'3 C values of methane range between -86‰ and -61 ‰. The δD values of methane range between -249‰ and -163‰. The C]/(C 2 + C 3 ) ratios of all samples are higher than 300. These data indicate methane is mainly produced by bacterial activity, and the primary methanogenic pathway is CO 2 reduction. The δ 13 C values of ethane range between -70‰ and -44%c. These values are among the lightest carbon isotope values reported for ethane in natural gas, indicating the origin of ethane is also predominantly bacterial. In deeper strata below 200 m below seafloor (mbsf) at Site 860, the δ 13 C values of ethane become heavier from -60‰ to -45‰ and the C]/C 3 ratios decrease with increasing depth. This indicates the presence of a small amount of thermogenic hydrocarbons. The low degree of maturation of the organic matter suggest the thermogenic hydrocarbon components are not generated in situ but have migrated from more mature strata, possibly driven by active fluid flow in these strata.

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“…In general, biogenic methane is isotopically light with methane δ 13 C values ranging from -55 to -90 permil (Rice and Claypool, 1981;Rice, 1993). However, biogenic methane can be as heavy as -40 permil, which can be produced by reduction of isotopically heavy carbon dioxide (Jenden and Kaplan, 1986). Thus, the gas from the Tyonek coal beds may be slightly biogenic but mostly thermogenic.…”

Section: Southern Alaska-cook Inlet Coal Provincementioning

confidence: 99%